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Molecular mapping of QTL for Fusarium head blight resistance introgressed into durum wheat

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Abstract

Key message

The major QTL for FHB resistance from hexaploid wheat line PI 277012 was successfully introgressed into durum wheat and minor FHB resistance QTL were detected in local durum wheat cultivars. A combination of these QTL will enhance FHB resistance of durum wheat.

Abstract

Fusarium head blight (FHB), caused by Fusarium graminearum, is a devastating disease of durum wheat. To combat the disease, great efforts have been devoted to introgress FHB resistance from its related tetraploid and hexaploid wheat species into adapted durum cultivars. However, most of the quantitative trait loci (QTL) for FHB resistance existing in the introgression lines are not well characterized or validated. In this study, we aimed to identify and map FHB resistance QTL in a population consisting of 205 recombinant inbred lines from the cross between Joppa (a durum wheat cultivar) and 10Ae564 (a durum wheat introgression line with FHB resistance derived from the hexaploid wheat line PI 277012). One QTL (Qfhb.ndwp-2A) from Joppa and two QTL (Qfhb.ndwp-5A and Qfhb.ndwp-7A) from 10Ae564 were identified through phenotyping of the mapping population for FHB severity and DON content in greenhouse and field and genotyping with 90K wheat Infinium iSelect SNP arrays. Qfhb.ndwp-2A explained 14, 15, and 9% of the phenotypic variation, respectively, for FHB severity in two greenhouse experiments and for mean DON content across the two greenhouse environments. Qfhb.ndwp-5A explained 19, 10, and 7% of phenotypic variation, respectively, for FHB severity in one greenhouse experiment, mean FHB severity across two field experiments, and mean DON content across the two greenhouse experiments. Qfhb.ndwp-7A was only detected for FHB severity in the two greenhouse experiments, explaining 9 and 11% of the phenotypic variation, respectively. This study confirms the existence of minor QTL in North Dakota durum cultivars and the successful transfer of the major QTL from PI 277012 into durum wheat.

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Acknowledgements

We thank Joe Mullins, Rui Wang, Subidhya Shrestha, Poudel, Liren Sun, and Qiang Li for assistance in greenhouse and field experiments. This material is based upon work supported by the US Department of Agriculture, under Agreement No. 59-0200-3-004. This is a cooperative project with the US Wheat & Barley Scab Initiative. Any opinions, findings, conclusions, or recommendations expressed in this publication are those of the author(s) and do not necessarily reflect the view of the US Department of Agriculture.

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Authors and Affiliations

  1. Department of Plant Pathology, North Dakota State University, Fargo, ND, 58108-6050, USA

    Mingxia Zhao, Yueqiang Leng & Shaobin Zhong

  2. USDA-ARS, Cereal Crops Research Unit, Red River Valley Agricultural Research Center, Fargo, ND, 58102, USA

    Shiaoman Chao & Steven S. Xu

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  1. Mingxia Zhao
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  2. Yueqiang Leng
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  3. Shiaoman Chao
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  4. Steven S. Xu
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  5. Shaobin Zhong
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Correspondence to Shaobin Zhong.

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The authors state that all experiments in the study comply with the ethical standards in the USA.

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Communicated by Thomas Miedaner.

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Fig. S1

Spike phenotypes of Joppa and 10Ae564. The plants were grown in the greenhouse. The spikes with awns trimmed were inoculated by point injection of Fusarium graminearum spores in a middle floret at anthesis. The spikes with the awns were not inoculated. Photos were taken 21 days after the inoculation (TIFF 14869 kb)

Fig. S2

Scatter plots of overall means for FHB severity in greenhouse experiments against those measured in the field experiments for the Jop10A population (PNG 15 kb)

Fig. S3

Scatter plots of overall means for FHB severity against DON content measured in greenhouse experiments for the Jop10A population (PNG 15 kb)

Fig. S4

Scatter plots of overall means for days to flower from planting against overall means for FHB severity measured in the field experiments for the Jop10A population (PNG 15 kb)

Fig. S5

Regions of linkage maps for chromosomes 4B harboring QTL for flowering date detected in the Jop10A population. The centiMorgan (cM) distances between marker loci and the positions of marker loci are on the left and right sides of the linkage maps, respectively. The LOD significance threshold 3.0 is represented by a vertical dotted line. 15FAR_DTF (solid black line) indicates the mean number of days from planting to flower measured in the field experiment conducted in 2015. The marker (IWB54805) most closely linked to the QTL is indicated by red color (TIFF 2539 kb)

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Zhao, M., Leng, Y., Chao, S. et al. Molecular mapping of QTL for Fusarium head blight resistance introgressed into durum wheat. Theor Appl Genet 131, 1939–1951 (2018). https://doi.org/10.1007/s00122-018-3124-4

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  • DOI: https://doi.org/10.1007/s00122-018-3124-4

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