Abstract
Key message
Four QTLs and an epistatic interaction were associated with disease severity in response to inoculation with Fusarium oxysporum f. sp. melonis race 1 in a recombinant inbred line population of melon.
Abstract
The USDA Cucumis melo inbred line, MR-1, harbors a wealth of alleles associated with resistance to several major diseases of melon, including powdery mildew, downy mildew, Alternaria leaf blight, and Fusarium wilt. MR-1 was crossed to an Israeli cultivar, Ananas Yok’neam, which is susceptible to all of these diseases, to generate a recombinant inbred line (RIL) population of 172 lines. In this study, the RIL population was genotyped to construct an ultra-dense genetic linkage map with 5663 binned SNPs anchored to the C. melo genome and exhibits the overall high quality of the assembly. The utility of the densely genotyped population was demonstrated through QTL mapping of a well-studied trait, resistance to Fusarium wilt caused by Fusarium oxysporum f. sp. melonis (Fom) race 1. A major QTL co-located with the previously validated resistance gene Fom-2. In addition, three minor QTLs and an epistatic interaction contributing to Fom race 1 resistance were identified. The MR-1 × AY RIL population provides a valuable resource for future QTL mapping studies and marker-assisted selection of disease resistance in melon.
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Acknowledgements
This research used resources provided by the SCINet project of the USDA Agricultural Research Service, ARS project number 0500-00093-001-00-D.
Funding
This study was funded, in part, by the United States Department of Agriculture (USDA) project number 6080-22000-028-00 and the National Institute of Food and Agriculture, Specialty Crops Research Initiative project number 6080-21000-018-08.
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The authors declare that they have no conflict of interest.
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The experiment conducted complies with the laws of the United States.
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Communicated by Sanwen Huang.
Electronic supplementary material
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122_2017_3039_MOESM1_ESM.csv
Supplementary material 1 (CSV 4 kb) Online resource 1 Disease severity line means (dsLineMeans), best linear unbiased predictors of disease severity (dsBLUPs), and line means within each test (dsTest1 and dsTest2) for the MR-1 x AY RIL population and parents
122_2017_3039_MOESM2_ESM.csv
Supplementary material 2 (CSV 150 kb) Online resource 2 Linkage groups (LG) and genetic positions (cM) of 5,747 binned SNPs (named as “S”chromosome”_“physical position in base pairs) prior to the removal of misaligned SNPs
122_2017_3039_MOESM3_ESM.csv
Supplementary material 3 (CSV 2180 kb) Online resource 3 Genotypes (AA indicates alleles contributed by MR-1 and BB by AY), linkage group (LG), genetic position (cM), chromosome (CS), physical position (Mb), and p value from a χ2 test of deviations from expected segregation ratios (P value) for the 5,663 binned SNPs used to generate the MR-1 x AY genetic linkage map
122_2017_3039_MOESM4_ESM.csv
Supplementary material 4 (CSV 58 kb) Online resource 4 Chromosome (cs), start and stop position (in bp), strand designation, and functional descriptors for genes found within the 1.5-LOD intervals of Fusarium oxysporum f.sp. melonis race 1 resistance QTLs
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Branham, S.E., Levi, A., Katawczik, M. et al. Construction of a genome-anchored, high-density genetic map for melon (Cucumis melo L.) and identification of Fusarium oxysporum f. sp. melonis race 1 resistance QTL. Theor Appl Genet 131, 829–837 (2018). https://doi.org/10.1007/s00122-017-3039-5
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DOI: https://doi.org/10.1007/s00122-017-3039-5