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Fine mapping of the chromosome 5B region carrying closely linked rust resistance genes Yr47 and Lr52 in wheat

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Fine mapping of Yr47 and Lr52 in chromosome arm 5BS of wheat identified close linkage of the marker sun180 to both genes and its robustness for marker-assisted selection was demonstrated.

Abstract

The widely effective and genetically linked rust resistance genes Yr47 and Lr52 have previously been mapped in the short arm of chromosome 5B in two F3 populations (Aus28183/Aus27229 and Aus28187/Aus27229). The Aus28183/Aus27229 F3 population was advanced to generate an F6 recombinant inbred line (RIL) population to identify markers closely linked with Yr47 and Lr52. Diverse genomic resources including flow-sorted chromosome survey sequence contigs representing the orthologous region in Brachypodium distachyon, the physical map of chromosome arm 5BS, expressed sequence tags (ESTs) located in the 5BS6-0.81-1.00 deletion bin and resistance gene analog contigs of chromosome arm 5BS were used to develop markers to saturate the target region. Selective genotyping was also performed using the iSelect 90 K Infinium wheat SNP assay. A set of SSR, STS, gene-based and SNP markers were developed and genotyped on the Aus28183/Aus27229 RIL population. Yr47 and Lr52 are genetically distinct genes that mapped 0.4 cM apart in the RIL population. The SSR marker sun180 co-segregated with Lr52 and mapped 0.4 cM distal to Yr47. In a high resolution mapping population of 600 F2 genotypes Yr47 and Lr52 mapped 0.2 cM apart and marker sun180 was placed 0.4 cM distal to Lr52. The amplification of a different sun180 amplicon (195 bp) than that linked with Yr47 and Lr52 (200 bp) in 204 diverse wheat genotypes demonstrated its robustness for marker-assisted selection of these genes.

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Acknowledgements

Naeela Qureshi acknowledges the University of Sydney for the USYDIS award. We thank the Grains Research and Development Corporation (GRDC) Australia for financial support through the Australian Cereal Rust Control Program.

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Authors and Affiliations

  1. Faculty of Agriculture, Food and Natural Resources, The University of Sydney Plant Breeding Institute, Private Bag 4011, Narellan, NSW, 2567, Australia

    Naeela Qureshi, Harbans Bariana & Urmil Bansal

  2. Department of Economic Development, Jobs, Transport and Resources, La Trobe University AgriBio, Bundoora, VIC, 3083, Australia

    Kerrie Forrest & Matthew Hayden

  3. Department of Plant and Microbial Biology, University of Zurich, Zollikerstrasse 107, 8008, Zurich, Switzerland

    Beat Keller & Thomas Wicker

  4. USDA-ARS Cereal Crops Research Unit, Red River Valley Agricultural Research Center, 1605 Albrecht BLVD, Fargo, ND, 58102-2765, USA

    Justin Faris

  5. Institute of Cytology and Genetics SB RAS, Novosibirsk, Russia

    Elena Salina

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  1. Naeela Qureshi
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  2. Harbans Bariana
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  3. Kerrie Forrest
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Correspondence to Urmil Bansal.

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Communicated by A Zhang.

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Qureshi, N., Bariana, H., Forrest, K. et al. Fine mapping of the chromosome 5B region carrying closely linked rust resistance genes Yr47 and Lr52 in wheat. Theor Appl Genet 130, 495–504 (2017). https://doi.org/10.1007/s00122-016-2829-5

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