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Molecular mapping and marker development for the Triticum dicoccoides-derived stripe rust resistance gene YrSM139-1B in bread wheat cv. Shaanmai 139

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YrSM139-1B maybe a new gene for effective resistance to stripe rust and useful flanking markers for marker-assisted selection were developed.

Abstract

Stripe rust, caused by Puccinia striiformis f. sp. tritici, is an important foliar disease of wheat. Two dominant stripe rust resistant genes YrSM139-1B and YrSM139-2D were pyramided in bread wheat cultivar Shaanmai 139; one from wild emmer and the other from Thinopyrum intermedium. Three near-isogenic F7:8 line pairs (contrasting RILs), N122-1013R/S, N122-185R/S, and N122-1812R/S, independently derived from different F2 plants and differing at the YrSM139-1B locus were generated from the cross Shaanmai 139 × Hu 901-19 through marker-assisted selection. A large F2:3 population from cross N122-1013R × N122-1013S tested for stripe rust response and subjected to analysis with markers in the 1BS10-0.5 bin region using SSR expressed sequence tags (EST) and site-specific sequence markers developed from the 90 K Illumina iSelect SNP array. Five EST–STS markers and four allele-specific PCR markers were mapped to the YrSM139-1B region. The 30.5 cM genetic map for YrSM139-1B consisted of nine markers, two of which were closer to YrSM139-1B than Xgwm273, which was used in producing the contrasting RIL pairs. Race response data and allelism tests showed that YrSM139-1B is different from Yr10, Yr15, and Yr24/26/CH42.

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Acknowledgments

This work was financially supported by the National Natural Science Foundation of China (31371612), the National Key Basic Research Program (2013CB127700), Scientific Research Program of Shaanxi province (2014K02-04-04), the Key Technologies R&D Program (2013BAD01B02-6), China and Shaanxi Postdoctoral Science Foundation (2014T70938), the innovation project of science and technology of Shaanxi province (2015KTZDNY01-01-02), and Cyrus Tang Foundation of America. We thank Dr. Robert McIntosh for critical review of a draft manuscript.

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Authors and Affiliations

  1. State Key Laboratory of Crop Stress Biology for Arid Areas, College of Agronomy (Northwest A&F University), Yangling, 712100, Shaanxi, China

    Hong Zhang, Lu Zhang, Changyou Wang, Yajuan Wang, Shikai Lv, Xinlun Liu & Wanquan Ji

  2. College of Plant Protection, Northwest A&F University, Yangling, 712100, Shaanxi, China

    Hong Zhang, Xinli Zhou & Zhensheng Kang

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  1. Hong Zhang
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  2. Lu Zhang
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  3. Changyou Wang
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  7. Xinlun Liu
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  9. Wanquan Ji
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Correspondence to Zhensheng Kang or Wanquan Ji.

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Communicated by F. Ordon.

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Zhang, H., Zhang, L., Wang, C. et al. Molecular mapping and marker development for the Triticum dicoccoides-derived stripe rust resistance gene YrSM139-1B in bread wheat cv. Shaanmai 139. Theor Appl Genet 129, 369–376 (2016). https://doi.org/10.1007/s00122-015-2633-7

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