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Zinc and iron concentration QTL mapped in a Triticum spelta × T. aestivum cross

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Ten QTL underlying the accumulation of Zn and Fe in the grain were mapped in a set of RILs bred from the cross Triticum spelta × T. aestivum . Five of these loci (two for Zn and three for Fe) were consistently detected across seven environments.

Abstract

The genetic basis of accumulation in the grain of Zn and Fe was investigated via QTL mapping in a recombinant inbred line (RIL) population bred from a cross between Triticum spelta and T. aestivum. The concentration of the two elements was measured from grain produced in three locations over two consecutive cropping seasons and from a greenhouse trial. The range in Zn and Fe concentration across the RILs was, respectively, 18.8–73.5 and 25.3–59.5 ppm, and the concentrations of the two elements were positively correlated with one another (rp =+0.79). Ten QTL (five each for Zn and Fe accumulation) were detected, mapping to seven different chromosomes. The chromosome 2B and 6A grain Zn QTL were consistently expressed across environments. The proportion of the phenotype explained (PVE) by QZn.bhu-2B was >16 %, and the locus was closely linked to the SNP marker 1101425|F|0, while QZn.bhu-6A (7.0 % PVE) was closely linked to DArT marker 3026160|F|0. Of the five Fe QTL detected, three, all mapping to chromosome 1A were detected in all seven environments. The PVE for QFe.bhu-3B was 26.0 %.

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Acknowledgments

The authors are grateful for constructive suggestions made by Wolfgang Pfeifer (HarvestPlus) and Ravi P. Singh and Hans J. Braun (CIMMYT, Mexico) in the course of this study. Support rendered by Uttam Kumar, CIMMYT in molecular analysis is also gratefully acknowledged.

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical standard

All experiments complied with the current laws of the India, the country in which they were performed.

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Authors and Affiliations

  1. Department of Genetics and Plant Breeding, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi, India

    Jayasudha Srinivasa, Balasubramaniam Arun, Vinod Kumar Mishra, Neeraj Kumar Vasistha & Arun Kumar Joshi

  2. Division of Genetics, Indian Agricultural Research Institute, Pusa, New Delhi, India

    Gyanendra Pratap Singh

  3. International Maize and Wheat Improvement Center (CIMMYT), Apdo. Postal 6-641, 06600, Mexico, D.F., Mexico

    Govindan Velu

  4. CIMMYT, India C/O ICRISAT, Patancheru, Hyderabad, India

    Raman Babu

  5. CIMMYT, South Asia Regional Office, Kathmandu, Nepal

    Arun Kumar Joshi

Authors
  1. Jayasudha Srinivasa
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  2. Balasubramaniam Arun
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  3. Vinod Kumar Mishra
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  4. Gyanendra Pratap Singh
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  5. Govindan Velu
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  7. Neeraj Kumar Vasistha
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  8. Arun Kumar Joshi
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Correspondence to Arun Kumar Joshi.

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Communicated by Yunbi Xu.

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Srinivasa, J., Arun, B., Mishra, V.K. et al. Zinc and iron concentration QTL mapped in a Triticum spelta × T. aestivum cross. Theor Appl Genet 127, 1643–1651 (2014). https://doi.org/10.1007/s00122-014-2327-6

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  • DOI: https://doi.org/10.1007/s00122-014-2327-6

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