Skip to main content
SpringerLink
Log in

Molecular mapping of two genes conferring resistance to Phytophthora sojae in a soybean landrace PI 567139B

  • Original Paper
  • Published:
Theoretical and Applied Genetics Aims and scope Submit manuscript

Abstract

Phytophthora root and stem rot (PRR), caused by the soil-borne oomycete pathogen Phytophthora sojae, is one of the most destructive diseases of soybean. PRR can be effectively controlled by race-specific genes conferring resistance to P. sojae (Rps). However, the Rps genes are usually non-durable, as populations of P. sojae are highly diverse and quick to adapt, and can be overcome 8–15 years after deployment. Thus, it is important to identify novel Rps genes for development of resistant soybean cultivars. PI 567139B is a soybean landrace carrying excellent resistance to nearly all predominant P. sojae races in Indiana. A mapping population consisting of 245 F2 individuals and 403 F2:3 families was developed from a cross between PI 567139B and the susceptible cultivar ‘Williams’, and used to dissect the resistance carried by PI 567139B. We found that the resistance in PI 567139B was conferred by two independent Rps genes, designated RpsUN1 and RpsUN2. The former was mapped to a 6.5 cM region between SSR markers Satt159 and BARCSOYSSR_03_0250 that spans the Rps1 locus on chromosome 3, while the latter was mapped to a 3.0 cM region between BARCSOYSSR_16_1275 and Sat_144, approximately 3.0–3.4 cM upstream of Rps2 on chromosome 16. According to the ‘Williams 82’ reference genome sequence, both regions are highly enriched with NBS-LRR genes. Marker assisted resistance spectrum analyses of these genes with 16 isolates of P. sojae, in combination with the mapping results, suggested that RpsUN1 was likely to be a novel allele at the Rps1 locus, while RpsUN2 was more likely to be a novel Rps gene.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  • Abney TS, Melgar JC, Richards TL, Scott DH, Grogan J, Young J (1997) New races of Phytophthora sojae with Rps1-d virulence. Plant Dis 81:653–655

    Article  Google Scholar 

  • Anderson TR, Buzzell RI (1992) Inheritance and linkage of the Rps7 gene for resistance to Phytophthora rot of soybean. Plant Dis 76:958–959

    Article  Google Scholar 

  • Bhattacharyya MK, Narayanan NN, Gao H, Santra DK, Salimath SS, Kasuga T, Liu Y, Espinosa B, Ellison L, Marek L, Shoemaker R, Gijzen M, Buzzell RI (2005) Identification of a large cluster of coiled coil-nucleotide binding site–leucine rich repeat-type genes from the Rps1 region containing Phytophthora resistance genes in soybean. Theor Appl Genet 111:75–86

    Article  CAS  PubMed  Google Scholar 

  • Cregan PB (2003) Soybean_Composite_Genetic_Map_2003 http://soybeanbreederstoolbox.org/

  • Demirbas A, Rector BG, Lohnes DG, Fioritto RJ, Graef GL, Cregan PB, Shoemaker RC, Specht JE (2001) Simple sequence repeat markers linked to the soybean Rps genes for Phytophthora resistance. Crop Sci 41:1220–1227

    Article  CAS  Google Scholar 

  • Devine TE, Kilen TC, O’ Neill JJ (1991) Genetic linkage of the Phytophthora resistance gene Rps2 and the nodulation response gene Rj2 in soybean. Crop Sci 31:713–715

  • Diers BW, Mansur L, Imsande J, Shoemaker RC (1992) Mapping Phytophthora resistance loci in soybean with restriction fragment length polymorphism markers. Crop Sci 32:377–383

    Article  CAS  Google Scholar 

  • Dorrance AE, Jia H, Abney TS (2004) Evaluation of soybean differentials for their interaction with Phytophthora sojae. Plant Health Progress. doi:10.1094/PHP-2004-0309-01-RS

    Google Scholar 

  • Dorrance AE, D.Mills, Robertson AE, Draper MA, Giesler L, Tenuta A (2007) Phytophthora root and stem rot of soybean. Plant Health Instr. doi:10.1094/PHI-I-2007-0830-07

  • Dorrance AE, Berry SA, Anderson TR, Meharg C (2008) Isolation, storage, pathotype characterization, and evaluation of resistance for Phytophthora sojae in soybean. Plant Health Progress. doi:10.1094/PHP-2008-0118-01-DG

    Google Scholar 

  • Gao H, Bhattacharyya MK (2008) The soybean-Phytophthora resistance locus Rps1-k encompasses coiled coil-nucleotide binding-leucine rich repeat-like genes and repetitive sequences. BMC Plant Biol 8:29

    Article  PubMed  Google Scholar 

  • Gao H, Narayanan NN, Ellison L, Bhattacharyya MK (2005) Two classes of highly similar coiled coil-nucleotide binding-leucine rich repeat genes isolated from the Rps1-k locus encode Phytophthora resistance in Soybean. MPMI 18:1035–1045

    Article  CAS  PubMed  Google Scholar 

  • Gardner ME, Hymowitz T, Xu SJ, Hartman GL (2001) Physical map location of the Rps1-k allele in soybean. Crop Sci 41:1435–1438

    Article  CAS  Google Scholar 

  • Gordon SG, St. Martin SK, Dorrance AE (2006) Rps8 maps to a resistance gene rich region on soybean molecular Linkage Group F. Crop Sci 46:168–173

  • Graham MA, Marek LF, Shoemaker RC (2002a) Organization, expression and evolution of a disease resistance gene cluster in soybean. Genetics 162:1961–1977

    CAS  PubMed  Google Scholar 

  • Graham MA, Marek LF, Shoemaker RC (2002b) PCR sampling of disease resistance-like sequences from a disease resistance gene cluster in soybean. Theor Appl Genet 105:50–57

    Article  CAS  PubMed  Google Scholar 

  • Hanson PM, Nickell CD, Gray LE, Sebastian SA (1988) Identification of two dominant genes conditioning brown stem rot resistance in soybean. Crop Sci 28:41–43

    Article  Google Scholar 

  • Kasuga T, Salimath SS, Shi J, Gijzen M, Buzzell RI, Bhattacharyya MK (1997) High resolution genetic and physical mapping of molecular markers linked to the Phytophthora resistance gene Rps1-k in soybean. MPMI 10:1035–1044

    Article  CAS  Google Scholar 

  • Leister D (2004) Tandem and segmental gene duplication and recombination in the evolution of plant disease resistance genes. Trends Genet 20:116–122

    Article  CAS  PubMed  Google Scholar 

  • Lohnes DG, Wagner RE, Bernard RL (1993) Soybean genes Rj2, Rmd, and Rps2 in linkage group 19. J Hered 84:109–111

    CAS  Google Scholar 

  • Michelmore RW, Meyers BC (1998) Clusters of resistance genes in plants evolve by divergent selection and a birth-and-death process. Genome Res 8:1113–1130

    CAS  PubMed  Google Scholar 

  • Mideros S, Nita M, Dorrance AE (2007) Characterization of components of partial resistance, Rps2, and root resistance to Phytophthora sojae in Soybean. Phytopathology 97:655–662

    Article  PubMed  Google Scholar 

  • Polzin KM, Lohnes DG, Nickell CD, Shoemaker RC (1994) Integration of Rps2, Rmd, and Rj2 into linkage group J of the soybean molecular map. J Hered 85:300–303

    CAS  Google Scholar 

  • Sandhu D, Gao H, Cianzio S, Bhattacharyya MK (2004) Deletion of a disease resistance nucleotide-binding-site leucine-rich- repeat-like sequence is associated with the loss of the Phytophthora resistance gene Rps4 in soybean. Genetics 168:2157–2167

    Article  CAS  PubMed  Google Scholar 

  • Sandhu D, Schallock KG, Rivera-Velez N, Lundeen P, Cianzio S, Bhattacharyya MK (2005) Soybean phytophthora resistance gene Rps8 maps closely to the Rps3 region. J Hered 96:536–541

    Article  CAS  PubMed  Google Scholar 

  • Schmitthenner AF (1985) Problems and progress in control of Phytophthora root rot of soybean. Plant Dis 69:362–368

    Article  Google Scholar 

  • Schmutz J, Cannon SB, Schlueter J, Ma J, Mitros T, Nelson W, Hyten DL, Song Q, Thelen JJ, Cheng J, Xu D, Hellsten U, May GD, Yu Y, Sakurai T, Umezawa T, Bhattacharyya MK, Sandhu D, Valliyodan B, Lindquist E, Peto M, Grant D, Shu S, Goodstein D, Barry K, Futrell-Griggs M, Abernathy B, Du J, Tian Z, Zhu L, Gill N, Joshi T, Libault M, Sethuraman A, Zhang XC, Shinozaki K, Nguyen HT, Wing RA, Cregan P, Specht J, Grimwood J, Rokhsar D, Stacey G, Shoemaker RC, Jackson SA (2010) Genome sequence of the palaeopolyploid soybean. Nature 463:178–183

    Article  CAS  PubMed  Google Scholar 

  • Smith D, Abney TS, Westphal A (2007) Phytophthora resistance of soybean germplasm with high potential for Asian soybean rust resistance. In: Southern soybean conference proceedings February 18, 2007, St. Louis, p 6

  • Song Q, Jia G, Zhu Y, Grant D, Nelson RT, Hwang E, Hyten DL, Cregan PB (2010) Abundance of SSR motifs and development of candidate polymorphic SSR markers (BARCSOYSSR_1.0) in soybean. Crop Sci 50:1950–1960

    Article  CAS  Google Scholar 

  • Sugimoto T, Yoshida S, Watanabe K, Aino M, Kanto T, Maekawa K, Irie K (2008) Identification of SSR markers linked to the Phytophthora resistance gene Rps1-d in soybean. Plant Breed 127:154–159

    Article  CAS  Google Scholar 

  • Sugimoto T, Yoshida S, Kaga A, Hajika M, Watanabe K, Aino M, Tatsuda K, Yamamoto R, Matoh T, Walker DR, Biggs AR, Ishimoto M (2011) Genetic analysis and identification of DNA markers linked to a novel Phytophthora sojae resistance gene in the Japanese soybean cultivar Waseshiroge. Euphytica 182:133–145

    Article  CAS  Google Scholar 

  • Sugimoto T, Kato M, Yoshida S, Matsumoto I, Kobayashi T, Kaga A, Hajika M, Yamamoto R, Watanabe K, Aino M, Matoh T, Walker DR, Biggs AR, Ishimoto M (2012) Pathogenic diversity of Phytophthora sojae and breeding strategies to develop Phytophthora-resistant soybeans. Breed Sci 61:511–522

    Article  CAS  PubMed  Google Scholar 

  • Sun S, Wu X, Zhao J, Wang Y, Tang Q, Yu D, Gai J, Xing H (2011) Characterization and mapping of RpsYu25, a novel resistance gene to Phytophthora sojae. Plant Breed 130:139–143

    Article  CAS  Google Scholar 

  • Van Ooijen JW, Voorrips RE (2001) JoinMap 3.0 software for the calculation of genetic linkage maps. Plant Research International, Wageningen, the Netherlands

  • Weng C, Yu K, Anderson TR, Poysa V (2001) Mapping genes conferring resistance to Phytophthora root rot of soybean, Rps1a and Rps7. J Hered 92:442–446

    Article  CAS  PubMed  Google Scholar 

  • Wrather JA, Koenning SR (2006) Estimates of disease effects on soybean yields in the United States 2003 to 2005. J Nematol 38:173–180

    PubMed  Google Scholar 

  • Wu X, Zhang B, Sun S, Zhao J, Yang F, Guo N, Gai J, Xing H (2011) Identification, genetic analysis and mapping of resistance to Phytophthora sojae of Pm28 in soybean. Agric Sci China 10:1506–1511

    Article  CAS  Google Scholar 

  • Yang S, Tang F, Gao M, Krishnan HB, Zhu H (2010) R gene-controlled host specificity in the legume-rhizobia symbiosis. Proc Natl Acad Sci USA 107:18735–18740

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

This work was mainly supported by soybean checkoff funds from the Indiana Soybean Alliance (Grant No. 205267), and partially supported by Agricultural Alumni Seed Improvement Association and the Purdue University Agricultural Research Program. We also thank Elisa Hughes, Brittany Radke, Tomara J. Fleury and Brian Foss for their help in inoculation and preparation of inoculum.

Author information

Authors and Affiliations

  1. Department of Agronomy, Purdue University, West Lafayette, IN, 47907, USA

    Feng Lin, Meixia Zhao, Jieqing Ping, Austin Johnson, Biao Zhang & Jianxin Ma

  2. Department of Botany and Plant Pathology, Purdue University, West Lafayette, IN, 47907, USA

    T. Scott Abney

  3. USDA-ARS Crop Production and Pest Control Research Unit, Purdue University, West Lafayette, IN, 47907, USA

    T. Scott Abney & Teresa J. Hughes

Authors
  1. Feng Lin
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Meixia Zhao
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Jieqing Ping
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Austin Johnson
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Biao Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. T. Scott Abney
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Teresa J. Hughes
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Jianxin Ma
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Teresa J. Hughes or Jianxin Ma.

Additional information

Communicated by H. T. Nguyen.

Electronic supplementary material

Below is the link to the electronic supplementary material.

122_2013_2127_MOESM1_ESM.xlsx

Genotype and reaction pattern of 403 F2:3 mapping families to isolates pmg(17)-1 and pmg(25)-1 of P. sojae. Supplementary material 1 (XLSX 61 kb)

122_2013_2127_MOESM2_ESM.xlsx

Marker assisted resistance spectrum analysis of RpsUN1 and RpsUN2 using selected F2:3 families. Supplementary material 2 (XLSX 46 kb)

Allelism tests of RpsUN1 and Rps1-k. Supplementary material 3 (XLSX 15 kb)

Rights and permissions

Reprints and permissions

About this article

Cite this article

Lin, F., Zhao, M., Ping, J. et al. Molecular mapping of two genes conferring resistance to Phytophthora sojae in a soybean landrace PI 567139B. Theor Appl Genet 126, 2177–2185 (2013). https://doi.org/10.1007/s00122-013-2127-4

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00122-013-2127-4

Keywords

Search

Navigation