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Identification of genomic regions involved in resistance against Sclerotinia sclerotiorum from wild Brassica oleracea

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Abstract

The lack of resistant source has greatly restrained resistance breeding of rapeseed (Brassica napus, AACC) against Sclerotinia sclerotiorum which causes severe yield losses in rapeseed production all over the world. Recently, several wild Brassica oleracea accessions (CC) with high level of resistance have been identified (Mei et al. in Euphytica 177:393–400, 2011), bringing a new hope to improve Sclerotinia resistance of rapeseed. To map quantitative trait loci (QTL) for Sclerotinia resistance from wild B. oleracea, an F2 population consisting of 149 genotypes, with several clones of each genotypes, was developed from one F1 individual derived from the cross between a resistant accession of wild B. oleracea (B. incana) and a susceptible accession of cultivated B. oleracea var. alboglabra. The F2 population was evaluated for Sclerotinia reaction in 2009 and 2010 under controlled condition. Significant differences among genotypes and high heritability for leaf and stem reaction indicated that genetic components accounted for a large portion of the phenotypic variance. A total of 12 QTL for leaf resistance and six QTL for stem resistance were identified in 2 years, each explaining 2.2–28.4 % of the phenotypic variation. The combined effect of alleles from wild B. oleracea reduced the relative susceptibility by 22.5 % in leaves and 15 % in stems on average over 2 years. A 12.8-cM genetic region on chromosome C09 of B. oleracea consisting of two major QTL intervals for both leaf and stem resistance was assigned into a 2.7-Mb genomic region on chromosome A09 of B. rapa, harboring about 30 putative resistance-related genes. Significant negative corrections were found between flowering time and relative susceptibility of leaf and stem. The association of flowering time with Sclerotinia resistance is discussed.

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Acknowledgments

We thank Prof. Wolfgang Friedt and Prof. Jinguo Hu for kindly discussion and comments on the manuscript. This study was funded by grants from Special Fund for Agroscientific Research in the Public Interest (201103016), NSFC (31171585), CSTC (201180001), 863 (2010BAD01B02), 111 project (B12006), the open funds of Ministry of Agriculture Key Laboratory of Oil Crops Biology, and Southwest University Scientific and Technological Innovation Fund to Graduates (kb2009006).

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Authors and Affiliations

  1. College of Agronomy and Biotechnology, Southwest University, Chongqing, 400716, China

    Jiaqin Mei, Yijuan Ding, Kun Lu, Dayong Wei, Yao Liu, Joseph Onwusemu Disi, Jiana Li, Liezhao Liu & Wei Qian

  2. Oil Crop Institute, China Academy of Agricultural Science, Wuhan, 430062, China

    Shengyi Liu

  3. Department of Bioagricultural Science and Pest Management, Colorado State University, Fort Collins, CO, 80523, USA

    John McKay

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  1. Jiaqin Mei
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  2. Yijuan Ding
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  3. Kun Lu
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Correspondence to Wei Qian.

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Communicated by A. Bervillé.

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Mei, J., Ding, Y., Lu, K. et al. Identification of genomic regions involved in resistance against Sclerotinia sclerotiorum from wild Brassica oleracea . Theor Appl Genet 126, 549–556 (2013). https://doi.org/10.1007/s00122-012-2000-x

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