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Mapping and candidate-gene screening of the novel Turnip mosaic virus resistance gene retr02 in Chinese cabbage (Brassica rapa L.)

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Abstract

The extreme resistance to Turnip mosaic virus observed in the Chinese cabbage (Brassica rapa) line, BP8407, is monogenic and recessive. Bulked segregant analysis was carried out to identify simple sequence repeat and Indel markers linked to this recessive resistance gene, termed recessive Turnip mosaic virus resistance 02 (retr02). Mapping of PCR-specific Indel markers on 239 individuals of a BP8407 × Ji Zao Chun F2 population, located this resistance gene to a 0.9-cM interval between two Indel markers (BrID10694 and BrID101309) and in scaffold000060 or scaffold000104 on chromosome A04 of the B. rapa genome. Eleven eukaryotic initiation factor 4E (eIF4E) and 14 eukaryotic initiation factor 4G (eIF4G) genes are predicted in the B. rapa genome. A candidate gene, Bra035393 on scaffold000104, was predicted within the mapped resistance locus. The gene encodes the eIF(iso)4E protein. Bra035393 was sequenced in BP8407 and Ji Zao Chun. A polymorphism (A/G) was found in exon 3 between BP8407 and Ji Zao Chun. This gene was analysed in four resistant and three susceptible lines. A correlation was observed between the amino acid substitution (Gly/Asp) in the eIF(iso)4E protein and resistance/susceptibility. eIF(iso)4E has been shown previously to interact with the TuMV genome-linked protein, VPg.

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Acknowledgments

We thank Dr. Bingyan Xie for providing the TuMV C4 isolate. This work was supported by Key Laboratory of Horticultural Crop Biology and Germplasm Innovation, Ministry of Agriculture.

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Authors and Affiliations

  1. Department of Chinese Cabbage, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, 100081, China

    Wei Qian, Shujiang Zhang, Shifan Zhang, Fei Li, Hui Zhang, Jian Wu, Xiaowu Wang & Rifei Sun

  2. School of Life Sciences, University of Warwick, Wellesbourne, Warwick, CV35 9EF, UK

    John A. Walsh

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  1. Wei Qian
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  2. Shujiang Zhang
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  3. Shifan Zhang
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  4. Fei Li
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  7. Xiaowu Wang
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Correspondence to Rifei Sun.

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Communicated by F. Ordon.

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Qian, W., Zhang, S., Zhang, S. et al. Mapping and candidate-gene screening of the novel Turnip mosaic virus resistance gene retr02 in Chinese cabbage (Brassica rapa L.) . Theor Appl Genet 126, 179–188 (2013). https://doi.org/10.1007/s00122-012-1972-x

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  • DOI: https://doi.org/10.1007/s00122-012-1972-x

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