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QTL mapping of resistance to gray leaf spot in maize

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Abstract

Gray leaf spot (GLS), caused by the causal fungal pathogen Cercospora zeae-maydis, is one of the most serious foliar diseases of maize worldwide. In the current study, a highly resistant inbred line Y32 and a susceptible line Q11 were used to produce segregating populations for both genetic analysis and QTL mapping. The broad-sense heritability (H 2) for GLS resistance was estimated to be as high as 0.85, indicating that genetic factors played key roles in phenotypic variation. In initial QTL analysis, four QTL, located on chromosomes 1, 2, 5, and 8, were detected to confer GLS resistance. Each QTL could explain 2.53–23.90 % of the total phenotypic variation, predominantly due to additive genetic effects. Two major QTL, qRgls1 and qRgls2 on chromosomes 8 and 5, were consistently detected across different locations and replicates. Compared to the previous results, qRgls2 is located in a ‘hotspot’ for GLS resistance; while, qRgls1 does not overlap with any other known resistance QTL. Furthermore, the major QTL-qRgls1 was fine-mapped into an interval of 1.4 Mb, flanked by the markers GZ204 and IDP5. The QTL-qRgls1 could enhance the resistance percentages by 19.70–61.28 %, suggesting its usefulness to improve maize resistance to GLS.

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Acknowledgments

We are grateful to Mr. Kun Wang, Shiqiang Li, and Fuyan Jiang and Miss Haiyan Li from Yunnan Academy of Agricultural Sciences for their technical assistance in the experimental field. We greatly appreciate the efforts of Prof. Siquan Shao, Xinyi Pang and Bihua Huang from Baoshan and Dehong Institute of Agricultural Sciences for their help in symptom diagnose in the field. This study was financially supported by the national ‘973’ basic research program; the national ‘863’ high-tech program of China; and the ‘outstanding PhD thesis foundation’ of Beijing municipal authorities.

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Authors and Affiliations

  1. National Maize Improvement Center of China, China Agricultural University, 2 West Yuanmingyuan Road, Haidian District, Beijing, 100193, People’s Republic of China

    Yan Zhang, Ling Xu & Mingliang Xu

  2. Institute of Food Crops, Yunnan Academy of Agricultural Sciences, Longtou Street, Kunming, 650205, People’s Republic of China

    Xingming Fan, Jing Tan & Wei Chen

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  1. Yan Zhang
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  2. Ling Xu
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  3. Xingming Fan
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  4. Jing Tan
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  5. Wei Chen
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  6. Mingliang Xu
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Correspondence to Mingliang Xu.

Additional information

Communicated by H. Geiger.

Y. Zhang and L. Xu contributed equally to this work.

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Zhang, Y., Xu, L., Fan, X. et al. QTL mapping of resistance to gray leaf spot in maize. Theor Appl Genet 125, 1797–1808 (2012). https://doi.org/10.1007/s00122-012-1954-z

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