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Map-based cloning proves qGC-6, a major QTL for gel consistency of japonica/indica cross, responds by Waxy in rice (Oryza sativa L.)

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Abstract

In this study, one major QTL affecting gel consistency (GC) of japonica/indica cross was identified on chromosome 6 using a DH population. To understand the molecular mechanism that regulates GC in rice grains, the major QTL (qGC-6) was isolated through a map-based cloning approach utilizing chromosome segment substitution lines (CSSLs). Using 64 plants with extremely soft GC that were selected on recombinant break points between two SSR markers, RM540 and RM8200 in a BC4F2 population, qGC-6 was mapped to a 60-kb DNA region between two STS markers, S26 and S27. These two markers were then used to further identify recombination break points. Finally, qGC-6 was delimited in an interval of a 11-kb region. Gene prediction analysis of the 11-kb DNA sequence containing qGC-6 identified only one putative ORF, which encodes granule-bound starch synthesis protein (Wx protein). Results of sequencing analysis and complementation experiment confirmed that this candidate ORF is responsible for rice GC. Genetic evidences revealed that Wx might contribute equally to the grain amylose content-controlling gene as well as gel consistency. This new information is important to breed rice varieties with improved grain quality.

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Acknowledgments

We thank Dr. Song Xianjun and Dr. Enrique R. Angeles for their critical comments on the manuscript. This work was supported by grants from the Ministry of Agriculture of China for transgenic research (No. 2011ZX08009, 2011ZX08001), the National Natural Science Foundation of China (No. 30771160, 30971760) and the Natural Science Foundation of the Zhejiang Province (No. R3090023) and Science and Technology Project, Zhejiang Province (2009C32047).

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Authors and Affiliations

  1. State Key Laboratory of Rice Biology, China National Rice Research Institute, 31006, Hangzhou, China

    Yan Su, Yuchun Rao, Shikai Hu, Yaolong Yang, Zhenyu Gao, Guanghen Zhang, Jian Liu, Jiang Hu, Meixian Yan, Guojun Dong, Li Zhu, Longbiao Guo, Qian Qian & Dali Zeng

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  1. Yan Su
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  2. Yuchun Rao
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  3. Shikai Hu
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  7. Jian Liu
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  11. Li Zhu
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  13. Qian Qian
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Corresponding authors

Correspondence to Qian Qian or Dali Zeng.

Additional information

Communicated by T. Sasaki.

Y. Su, Y. Rao and S. Hu contributed equally to this work.

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Su, Y., Rao, Y., Hu, S. et al. Map-based cloning proves qGC-6, a major QTL for gel consistency of japonica/indica cross, responds by Waxy in rice (Oryza sativa L.). Theor Appl Genet 123, 859–867 (2011). https://doi.org/10.1007/s00122-011-1632-6

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