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Development of a population for substantial new type Brassica napus diversified at both A/C genomes

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Abstract

Intersubgenomic heterosis in rapeseed has been revealed in previous studies by using traditional Brassica napus (AnAnCnCn) to cross partial new type B. napus with Ar/Cc introgression from the genomes of B. rapa and B. carinata, respectively. To further enlarge the genetic basis of B. napus and to facilitate a sustained heterosis breeding in rapeseed, it is crucial to create a population for substantial new type B. napus diversified at both A/C genomes. In this experiment, hundreds of artificial hexaploid plants (ArArBcBcCcCc) involving hundreds of B. carinata/B. rapa combinations were first crossed with elite lines of partial new type B. napus. The pentaploid plants (AABCC) were open-pollinated in isolated conditions, and their offspring were successively self-pollinated and intensively selected for two generations. Thereafter, a population of substantial new type B. napus mainly with a genomic composition of ArArCcCc harbouring genetic diversity from 25 original cultivars of B. rapa and 72 accessions of B. carinata was constructed. The population was cytologically verified to have the correct chromosome constitution of AACC and differed genetically from traditional B. napus, in terms of the genome components of Ar/Cc and Bc as well as the novel genetic variations induced by the interspecific hybridisation process. Synchronously, rich phenotypic variation with plenty of novel valuable traits was observed in the population. The origin of the novel variations and the value of the population are discussed.

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Acknowledgments

The authors are grateful to Professor Dr Zaiyun Li of Huazhong Agricultural University for his valuable help with the GISH technique, Dr Xingliang Liu for critical reading the manuscript. This study was supported by the National Natural Science Foundation of China (project code: 30830073) National 863 High Technology Program, P. R. China (2008AA10Z147).

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Authors and Affiliations

  1. National Key Laboratory of Crop Genetic Improvement, Huazhong Agriculture University, Wuhan, 430070, Hubei, China

    Yong Xiao, Lunlin Chen, Jun Zou, Entang Tian, Wei Xia & Jinling Meng

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  1. Yong Xiao
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  2. Lunlin Chen
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  3. Jun Zou
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  4. Entang Tian
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Correspondence to Jinling Meng.

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Communicated by H. Becker.

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Xiao, Y., Chen, L., Zou, J. et al. Development of a population for substantial new type Brassica napus diversified at both A/C genomes. Theor Appl Genet 121, 1141–1150 (2010). https://doi.org/10.1007/s00122-010-1378-6

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