Skip to main content
SpringerLink
Log in

Mapping quantitative trait loci for preharvest sprouting resistance in white wheat

  • Original Paper
  • Published:
Theoretical and Applied Genetics Aims and scope Submit manuscript

Abstract

The premature germination of seeds before harvest, known as preharvest sprouting (PHS), is a serious problem in all wheat growing regions of the world. In order to determine genetic control of PHS resistance in white wheat from the relatively uncharacterized North American germplasm, a doubled haploid population consisting of 209 lines from a cross between the PHS resistant variety Cayuga and the PHS susceptible variety Caledonia was used for QTL mapping. A total of 16 environments were used to detect 15 different PHS QTL including a major QTL, QPhs.cnl-2B.1, that was significant in all environments tested and explained from 5 to 31% of the trait variation in a given environment. Three other QTL QPhs.cnl-2D.1, QPhs.cnl-3D.1, and QPhs.cnl-6D.1 were detected in six, four, and ten environments, respectively. The potentially related traits of heading date (HD), plant height (HT), seed dormancy (DOR), and rate of germination (ROG) were also recorded in a limited number of environments. HD was found to be significantly negatively correlated with PHS score in most environments, likely due to a major HD QTL, QHd.cnl-2B.1, found to be tightly linked to the PHS QTL QPhs.cnl-2B.1. Using greenhouse grown material no overlap was found between seed dormancy and the four most consistent PHS QTL, suggesting that greenhouse environments are not representative of field environments. This study provides valuable information for marker-assisted breeding for PHS resistance, future haplotyping studies, and research into seed dormancy.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

Similar content being viewed by others

References

  • Anderson JA, Ogihara Y, Sorrells ME, Tanksley SD (1992) Development of a chromosomal arm map for wheat based on RFLP markers. Theor Appl Genet 83:1035–1043

    CAS  Google Scholar 

  • Anderson JA, Sorrells ME, Tanksley SD (1993) RFLP analysis of genomic regions associated with resistance to preharvest sprouting in wheat. Crop Sci 33:453–459

    CAS  Google Scholar 

  • Bailey PC, Mckibbin RS, Lenton JR, Holdsworth MJ, Flintham JE, Gale MD (1999) Genetic map locations for orthologous Vp1 genes in wheat and rice. Theor Appl Genet 98:281–284

    Article  CAS  Google Scholar 

  • Basten CJ, Weir BS, Zeng ZB (1994) Zmap—a QTL Cartographer. In: Smith C, Gavora JS, Benkel B, Chesnais J, Fairfull W, Gibson JP et al (eds) Proceedings of the 5th World congress on genetics applied to livestock production: Computing Strategies and Software, Guelph, Ontario, Canada

  • Basten CJ, Weir BS, Zeng ZB (2002) QTL Cartographer. Department of Statistics, North Carolina State University, Raleigh, NC

    Google Scholar 

  • Beales J, Turner A, Griffiths S, Snape JW, Laurie DA (2007) A pseudo-response regulator is misexpressed in the photoperiod insensitive Ppd-D1a mutant of wheat (Triticum aestivum L.). Theor Appl Genet 115:721–733

    Article  PubMed  CAS  Google Scholar 

  • Bewley JD, Black M (1994) Seeds:Physiology of Development and Germination. Plenum Press, New York

    Google Scholar 

  • Churchill GA, Doerge RW (1994) Empirical threshold values for quantitative trait mapping. Genetics 138:963–971

    PubMed  CAS  Google Scholar 

  • Ellis H, Spielmeyer W, Gale R, Rebetzke J, Richards A (2002) “Perfect” markers for the Rht-B1b and Rht-D1b dwarfing genes in wheat. Theor Appl Genet 105:1038–1042

    Article  PubMed  CAS  Google Scholar 

  • Flintham J (2000) Different genetic components control coat-imposed and embryo-imposed dormancy in wheat. Seed Sci Res 10:43–50

    Google Scholar 

  • Flintham J, Adlam R, Bassoi M, Holdsworth M, Gale M (2002) Mapping genes for resistance to sprouting damage in wheat. Euphytica 126:39–45

    Article  CAS  Google Scholar 

  • Groos C, Gay G, Perretant M, Gervais L, Bernard M, Dedryver F et al (2002) Study of the relationship between pre-harvest sprouting and grain color by quantitative trait loci analysis in a white × red grain bread-wheat cross. Theor Appl Genet 104:39–47

    Article  PubMed  CAS  Google Scholar 

  • Gupta PK, Balyan HS, Edwards KJ, Isaac P, Korzun V, Röder M et al (2002) Genetic mapping of 66 new microstalleite (SSR) loci in bread wheat. Theor Appl Genet 105:413–422

    Article  PubMed  CAS  Google Scholar 

  • Guyomarc’h H, Charmet G, Edwards KJ, Bernard M (2002) Characterisation of polymorphic microsatellite markers from Aegilops tauschii and transferability to the D-genome of bread wheat. Theor Appl Genet 104:1164–1172

    Article  PubMed  CAS  Google Scholar 

  • Hanocq E, Niarquin M, Heumez E, Rousset M, Le Gouis J (2004) Detection and mapping of QTL for earliness components in a bread wheat recombinant inbred lines population. Theor Appl Genet 110:106–115

    Article  PubMed  CAS  Google Scholar 

  • Heun M, Kennedy AE, Anderson JA, Lapitan NLV, Sorrells ME, Tanksley SD (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare). Genome 34:437–447

    Google Scholar 

  • Imtiaz M, Ogbonnaya FC, Oman J, Van Ginkel M (2008) Characterization of quantitative trait loci controlling genetic variation for preharvest sprouting in synthetic backcross-derived wheat lines. Genetics 178:1725–1736

    Article  PubMed  CAS  Google Scholar 

  • Kato K, Nakamura W, Tabiki T, Miura H (2001) Detection of loci controlling seed dormancy on group 4 chromosomes of wheat and comparative mapping with rice and barley genomes. Theor Appl Genet 10:980–985

    Article  Google Scholar 

  • King RW, Richards RA (1984) Water uptake in relation to pre-harvest sprouting damage in wheat:Ear characteristics. Aust J Agric Res 35:327–336

    Article  Google Scholar 

  • King RW, Von Wettstein-Knowles P (2000) Epicuticular waxes and regulation of ear wetting and pre-harvest sprouting in barley and wheat. Euphytica 112:157–166

    Article  Google Scholar 

  • Kulwal PL, Singh R, Balyan HS, Gupta PK (2004) Genetic basis of pre-harvest sprouting tolerance using single-locus and two-locus QTL analyses in bread wheat. Funct Integr Genomics 4:94–101

    Article  PubMed  CAS  Google Scholar 

  • Kulwal PL, Kumar N, Gaur A, Khurana P, Khurana JP, Tyagi AK et al (2005) Mapping of a major QTL for pre-harvest sprouting tolerance on chromosome 3A in bread wheat. Theor Appl Genet 111:1052–1059

    Article  PubMed  CAS  Google Scholar 

  • Liu ZH, Anderson JA, Hu J, Friesen TL, Rasmussen JB, Faris JD (2005) A wheat intervarietal genetic linkage map based on microsatellite and target region amplified polymorphism markers and its utility for detecting quantitative trait loci. Theor Appl Genet 111:782–794

    Article  PubMed  CAS  Google Scholar 

  • Liu S, Cai S, Graybosch R, Chen C, Bai G (2008) Quantitative trait loci for resistance to pre-harvest sprouting in US hard white winter wheat Rio Blanco. Theor Appl Genet 117:691–699

    Article  PubMed  CAS  Google Scholar 

  • Mangin B, Goffinet B, Rebai A (1994) Constructing confidence intervals for QTL location. Genetics 138:1301–1308

    PubMed  CAS  Google Scholar 

  • Manly KF, Cudmore RH, Meer M (2001) Map manager QTX, cross-platform software for genetic mapping. Mamm Genome 12:930–932

    Article  PubMed  CAS  Google Scholar 

  • Mares DJ, Mrva K (2001) Mapping quantitative trait loci associated with variation in grain dormancy in Australian wheat. Aust J Agric Res 52:1257–1265

    Article  CAS  Google Scholar 

  • Mares DJ, Mrva K, Tan MK, Sharp P (2002) Dormancy in white-grained wheat:Progress towards identification of genes and molecular markers. Euphytica 126:47–53

    Article  CAS  Google Scholar 

  • Mares D, Mrva K, Cheong J, Williams K, Watson B, Storlie E et al (2005) A QTL located on chromosome 4A associated with dormancy in white- and red-grained wheats of diverse origin. Theor Appl Genet 111:1357–1364

    Article  PubMed  CAS  Google Scholar 

  • Miura H, Sato N, Kato K, Amano Y (2002) Detection of chromosomes carrying genes for seed dormancy of wheat using the backcross reciprocal monosomic method. Plant Breeding 121:394–399

    Article  Google Scholar 

  • Mori M, Uchino N, Chono M, Kato K, Miura H (2005) Mapping QTLs for grain dormancy on wheat chromosome 3A and the group 4 chromosomes, and their combined effect. Theor Appl Genet 110:1257–1266

    Article  Google Scholar 

  • Mrva K, Mares DJ (1996) Expression of late maturity alpha-amylase in wheat containing gibberellic acid insensitivity genes. Euphytica 88:69–76

    Article  CAS  Google Scholar 

  • Ogbonnaya FC, Imtiaz M, Depauw RM (2007) Haplotype diversity of preharvest sprouting QTLs in wheat. Genome 50:107–118

    Article  PubMed  CAS  Google Scholar 

  • Ogbonnaya FC, Imtiaz M, Ye G, Hearnden PR, Hernandez E, Eastwood RF et al (2008) Genetic and QTL analyses of seed dormancy and preharvest sprouting resistance in the wheat germplasm CN10955. Theor Appl Genet 116:891–902

    Article  PubMed  CAS  Google Scholar 

  • Osa M, Kato K, Mori M, Shindo C, Torada A, Miura H (2003) Mapping QTLs for seed dormancy and the Vp1 homologue on chromosome 3A in wheat. Theor Appl Genet 106:1491–1496

    PubMed  CAS  Google Scholar 

  • Pestsova E, Ganal MW, Röder MS (2000) Isolation and mapping of microsatellite markers specific for the D genome of bread wheat. Genome 43:689–697

    Article  PubMed  CAS  Google Scholar 

  • Röder MS, Korzun V, Wendehake K, Plaschke J, Tixier M, Leroy P et al (1998) A microsatellite map of wheat. Genetics 149:2007–2023

    PubMed  Google Scholar 

  • Roy J, Prasad M, Varshney R, Balyan H, Blake T, Dhaliwal HS et al (1999) Identification of a microsatellite on chromosomes 6B and a STS on 7D of bread wheat showing an association with preharvest sprouting tolerance. Theor Appl Genet 99:336–340

    Article  Google Scholar 

  • Rozen S, Skaletsky H (2000) Primer3 on the WWW for general users and for biologist programmers. Methods Mol Biol 132:365–386

    PubMed  CAS  Google Scholar 

  • Somers DJ, Isaac P, Edwards K (2004) A high-density microsatellite consensus map for bread wheat (Triticum aestivum L.). Theor Appl Genet 109:1105–1114

    Article  PubMed  CAS  Google Scholar 

  • Song QJ, Shi JR, Singh S, Fickus EW, Costa JM, Lewis J et al (2005) Development and mapping of microsatellite (SSR) markers in wheat. Theor Appl Genet 110:550–560

    Article  PubMed  CAS  Google Scholar 

  • Sourdille P, Gandon B, Chiquet V, Nicot N, Somers D, Murigneux A et al (2004) Wheat Genoplante SSR mapping data release:A new set of markers and comprehensive genetic and physical mapping data. Graingenes. http://wheat.pw.usda.gov

  • Tan MK, Sharp PJ, Lu MQ, Howes N (2006) Genetics of grain dormancy in a white wheat. Aust J Agr Res 57:1157–1165

    Article  CAS  Google Scholar 

  • Torada A, Amano Y (2002) Effect of seed coat color on seed dormancy in different environments. Euphytica 126:99–105

    Article  CAS  Google Scholar 

  • Torada A, Koike M, Ikeguchi S, Tsutsui I (2008) Mapping of a major locus controlling seed dormancy using backcrossed progenies in wheat (Triticum aestivum L.). Genome 51:426–432

    Article  PubMed  CAS  Google Scholar 

  • Vos P, Hogers R, Bleeker M, Reijans M, Van De Lee T et al (1995) AFLP:A new technique for DNA fingerprinting. Nucleic Acids Res 23:4407–4414

    Article  PubMed  CAS  Google Scholar 

  • Walker-Simmons M (1987) ABA levels and sensitivity in developing wheat embryos of sprouting resistant and susceptible cultivars. Plant Physiol 84:61–66

    Article  PubMed  CAS  Google Scholar 

  • Walker-Simmons M (1988) Enhancement of ABA responsiveness in wheat embryos by high temperature. Plant Cell Environ 11:769–775

    Article  CAS  Google Scholar 

  • Wu J, Carver BF, Goad CL (1999) Kernel color variability of hard white and hard red winter wheat. Crop Sci 39:634–638

    Article  Google Scholar 

  • Yang J, Zhu J, Williams RW (2007a) Mapping the genetic architecture of complex traits in experimental populations. Bioinformatics 23:1527–1536

    Article  PubMed  CAS  Google Scholar 

  • Yang Y, Zhao XL, Xia LQ, Chen XM, Xia XC, Yu Z et al (2007b) Development and validation of a Viviparous-1 STS marker for pre-harvest sprouting tolerance in Chinese wheats. Theor Appl Genet 115:971–980

    Article  PubMed  CAS  Google Scholar 

  • Yang J, Hu C, Hu H, Yu R, Xia Z, Ye X et al (2008) QTLNetwork:Mapping and visualizing genetic architecture of complex traits in experimental populations. Bioinformatics 24:721–723

    Article  PubMed  CAS  Google Scholar 

  • Yu J, Dake TM, Singh S, Benscher D, Li W, Gill B et al (2004) Development and mapping of EST-derived simple sequence repeat markers for hexaploid wheat. Genome 47:805–818

    Article  PubMed  CAS  Google Scholar 

  • Zanetti S, Winzeler M, Keller M, Keller B, Messmer M (2000) Genetic analysis of pre-harvest sprouting resistance in a wheat × spelt cross. Crop Sci 40:1406–1417

    CAS  Google Scholar 

Download references

Acknowledgments

This research was supported by National Research Initiative Competitive Grants 2005-35301-15728 and 2006-55606-16629 from the USDA Cooperative State Research, Education, and Extension Service and Hatch project, pp 149–419.

Author information

Author notes

  1. Jesse D. Munkvold

    Present address: Boyce Thompson Institute for Plant Research, Tower Road, Ithaca, NY, 14853, USA

Authors and Affiliations

  1. Department of Plant Breeding and Genetics, Cornell University, 240 Emerson Hall, Ithaca, NY, 14853, USA

    Jesse D. Munkvold, James Tanaka, David Benscher & Mark E. Sorrells

  2. Department of Plant Breeding and Genetics, Cornell University, 240 Emerson Hall, Ithaca, NY, 14853, USA

    Mark E. Sorrells

Authors
  1. Jesse D. Munkvold
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. James Tanaka
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. David Benscher
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Mark E. Sorrells
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Mark E. Sorrells.

Additional information

Communicated by I. Romagosa.

Electronic supplementary material

Rights and permissions

Reprints and permissions

About this article

Cite this article

Munkvold, J.D., Tanaka, J., Benscher, D. et al. Mapping quantitative trait loci for preharvest sprouting resistance in white wheat. Theor Appl Genet 119, 1223–1235 (2009). https://doi.org/10.1007/s00122-009-1123-1

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00122-009-1123-1

Keywords

Search

Navigation