Skip to main content
SpringerLink
Log in

Identification and molecular mapping of a Fusarium wilt resistant gene in upland cotton

  • Original Paper
  • Published:
Theoretical and Applied Genetics Aims and scope Submit manuscript

Abstract

Fusarium wilt (FW) is one of the most economically damaging cotton diseases worldwide, causing yellowing, wilting, defoliation, vascular tissue damage and ultimately death. Identification of molecular markers linked to FW genes is vital to incorporate resistance into elite cotton cultivars. An intraspecific F2 in Gossypium hirsutum L. was developed by crossing with a highly resistant cultivar Zhongmiansuo 35 (ZMS35) and a susceptible cultivar Junmian 1 to screen simple sequence repeats (SSRs) closely linked to the FW resistance gene. FW was identified in F2:3 families by evaluating seedling leaf symptoms and vascular tissue damage at plant maturity under natural field infection conditions over 2 years. The results showed that FW resistance segregated in a 3:1 ratio as a simple monogenic trait in F2:3 families. Molecular mapping identified a FW resistance gene closely linked with the SSR marker JESPR304−280 in chromosome D3(c17). We proposed to name this gene FW R. A composite interval mapping method detected four QTLs for FW resistance in Chr.A7(c7), D1(c15), D9(c23) and D3, respectively. Among them, one major QTL (LOD > 20) was tagged near marker JESPR304 within an interval of 0.06–0.2 cM, and explained over 52.5–60.9% of the total phenotypic variance. The data confirmed the existence of a major gene in Chr.D3. This is the first report of molecular mapping of a major gene contributing FW resistance in cotton. The present research therefore provides an opportunity to understand the genetic control of resistance to FW and conduct molecular marker-assisted selection breeding to develop FW resistant cultivars.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  • Armstrong GM, Armstrong JK (1980) Race 6 of the cotton-wilt Fusarium from Paragury. Plant Dis 64:596

    Google Scholar 

  • Basten CJ, Weir BS, Zeng ZB (2001) QTL Cartographer, Version 1.15. Department of Statistics. North Carolina State University, Raleigh

    Google Scholar 

  • Bezawada C, Saha S, Jenkins JN, Creech RG, McCarty JC (2003) SSR marker(s) associated with root-knot nematode resistance gene(s) in cotton. J Cotton Sci 7:179–184

    CAS  Google Scholar 

  • Bridge J (1992) Nematodes. In: Hollocks RJ (ed) Cotton diseases. CAB Int, Wallingford, pp 33–353

    Google Scholar 

  • Brotman Y, Kovalski I, Dogimont C, Pitrat M, Portnoy V, Katzir N, Perl-Treves R (2005) Molecular markers linked to papaya ring spot virus resistance and Fusarium race 2 resistance in melon. Theor Appl Genet 110:337–345

    Article  PubMed  CAS  Google Scholar 

  • Chen QY, Ji XQ, Sun WJ (1985) Identification of cotton-wilt Fusarium races in China. Sci Agri Sin 18(6):1–6

    Google Scholar 

  • Davis RD, Moore NY, Kochman JK (1996) Characterization of a population of Fusarium oxysporum f.sp. vasinfectum causing wilt of cotton in Australia. Aust J Agric Res 47:1143–1156

    Article  Google Scholar 

  • Feng CD, Zhang JF, Liu JL, Guo JH, Sun JZ (1996) Inheritance of resistance of Fusarium Wilt in several Chinese Upland cotton varieties. Sci Agri Sin 22:550–554 (in Chinese)

    Google Scholar 

  • Guo WZ, Cai CP, Wang CB, Han ZG, Song XL, Wang K, Niu XW, Wang C, Lu KY, Shi B, Zhang TZ (2007) A microsatellite-based, gene-rich linkage map reveals genome structure, function, and evolution in Gossypium. Genetics 176:527–541

    Article  PubMed  CAS  Google Scholar 

  • Hamwieh A, Udupa SM, Choumane W, Sarker A, Dreyer F, Jung C, Baum M (2005) A genetic linkage map of Lens sp. based on microsatellite and AFLP markers and the localization of Fusarium vascular wilt resistance. Theor Appl Genet 110:669–677

    Article  PubMed  CAS  Google Scholar 

  • Han ZG, Wang CB, Song XL, Guo WZ, Guo JY, Li CH, Chen XY, Zhang TZ (2006) Characteristics, development and mapping of Gossypium hirsutum derived EST-SSR in allotetraploid cotton. Theor Appl Genet 112:430–439

    Article  PubMed  CAS  Google Scholar 

  • Hemming MN, Basuki S, McGrath DJ, Carroll BJ, Jones DA (2004) Fine mapping of the tomato I-3 gene for Fusarium wilt resistance and elimination of a co-segregating resistance gene analogue as a candidate for I-3. Theor Appl Genet 109:409–418

    Article  PubMed  CAS  Google Scholar 

  • Hillocks RJ (1984) Production of cotton varieties with resistance to Fusarium wilt with special reference to Tanzania. Trop Pest Manage 30:234–246

    Article  Google Scholar 

  • Huang ZK (2007) China cotton varieties and family tree. China Agri Sci Press, Beijing (in Chinese)

    Google Scholar 

  • Hyer AH, Jorgenson EC, Garber RH, Smith S (1979) Resistance to root-knot nematode in control of root-knot nematode–Fusarium wilt disease complex in cotton. Crop Sci 19:898–901

    Article  Google Scholar 

  • Katsantonis D, Hillocks RJ, Gowen S (2003) Comparative effect of root-knot nematode on severity of Verticillium and Fusarium wilt in cotton. Phytoparasitica 31:154–162

    Article  Google Scholar 

  • Kohel RJ (1973) Genetic nomenclature in cotton. J Hered 64:291–295

    Google Scholar 

  • Lander ES, Green P, Abrahamson J, Barlow A, Daly MJ, Lincoln SE, Newburg L (1987) Mapmaker: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1:174–181

    Article  PubMed  CAS  Google Scholar 

  • Ma C, Chen QY (1992) Progress on studies of cotton resistant breeding Fusarium and Verticillium wilts in China. Sci Agri Sin 25:50–57

    Google Scholar 

  • Ma C, Jian GL, Zheng CL (2002) The Advances in cotton breeding resistance to Fusarium and Verticillium wilts in China during past fifty years. Sci Agri Sin 35:508–513

    Google Scholar 

  • McCouch SR, Cho YG, Yano PE, Blinstrub M, Morishima H, Kinoshita T (1997) Report on QTL nomenclature. Rice Genet Newslett 14:11–13

    Google Scholar 

  • McPherson MG, Jenkins JN, Watson CE, McCarty JC (2004) Inheritance of root-knot nematode resistance in M-315 RNR and M78-RNR cotton. J Cotton Sci 8:154–161

    Google Scholar 

  • Mutlu N, Boyacı FH, Göçmen M, Abak K (2008) Development of SRAP, SRAP-RGA, RAPD and SCAR markers linked with a Fusarium wilt resistance gene in eggplant. Theor Appl Genet 117:1303–1312

    Article  PubMed  CAS  Google Scholar 

  • Ogallo JL, Goodell PB, Eckert J, Roberts PA (1997) Evaluation of NemX, a new cultivar of cotton with high resistance to Meloidogyne incognita. J Nematol 29:531–537

    PubMed  CAS  Google Scholar 

  • Paterson AH, Brubaker CL, Wendel JF (1993) A rapid method for extraction of cotton (Gossypium Spp.) genomic DNA suitable for RFLP or PCR analysis. Plant Mol Bio Rep 11:122–127

    Article  CAS  Google Scholar 

  • Sharma KD, Winter P, Kahl G, Muehlbauer FJ (2004) Molecular mapping of Fusarium oxysporum f. sp. ciceris race 3 resistance gene in chickpea. Theor Appl Genet 108:1243–1248

    Article  PubMed  CAS  Google Scholar 

  • Shen XL, Becelaere GV, Kumar P, Davis RF, May OL, Chee P (2006) QTL mapping for resistance to root-knot nematodes in the M-120 RNR Upland cotton line (Gossypium hirsutum L.) of the Auburn 623 RNR source. Theor Appl Genet 113:1539–1549

    Article  PubMed  CAS  Google Scholar 

  • Shepherd RL (1970) Breeding for resistance to the root knot fusarium wilt complex in cotton. In: Proceedings of Beltwide cotton production research conferences, Houston, TX. pp 68

  • Shi LY (1996) Studies on the pathogen of Fusarium vasinfectum and Verticillium dahliae of cotton. Acta Gossypii Sin 8:292–294

    Google Scholar 

  • Simons G, Groenendijk J, Wijbrandi J, Reijans M, Groenen J, Diergaarde P, Van der Lee T, Bleeker M, Onstenk J, de Both M, Haring M, Mes J, Cornelissen B, Zabeau M, Vos P (1998) Dissection of the Fusarium I2 gene cluster in tomato reveals six homologs and one active gene copy. Plant Cell 10:1055–1068

    Article  PubMed  CAS  Google Scholar 

  • Smith AL, Dick JB (1960) Inheritance of resistance to Fusarium wilt in Upland and cotton Sea Island cotton as complicated by nematodes under field conditions. Phytopathology 50:44–48

    Google Scholar 

  • Swetha PR, Subramanian RB (2008) Isolation and molecular analysis of R-gene in resistant Zingiber officinale (ginger) varieties against Fusarium oxysporum f.sp. zingiberi. Bioresour Technol 99:4540–4543

    Article  CAS  Google Scholar 

  • Ulloa M, Hutmacher RB, Davis RM, Wright SD, Percy R, Marsh B (2006) Breeding for fusarium wilt Race 4 resistance in cotton under field and greenhouse conditions. J Cotton Sci 10:114–127

    Google Scholar 

  • Wang YH, Thomas CE, Ralph AD (2000) Genetic mapping of a Fusarium wilt resistance gene (Fom-2) in melon (Cucumis melo L). Mol Breed 6:379–389

    Article  CAS  Google Scholar 

  • Wang YH, Choi WB, Thomas E, Dean RA (2002) Cloning of disease-resistance homologues in end sequences of BAC clones linked to Fom-2, a gene conferring resistance to Fusarium wilt in melon (Cucumis melo L.). Genome 45:473–480

    Article  PubMed  CAS  Google Scholar 

  • Wang C, Ulloa M, Roberts P (2006a) Identification and mapping of microsatellite markers linked to a root-knot nematode resistance gene (rkn1) in Acala NemX cotton. Theor Appl Genet 112:770–777

    Article  PubMed  CAS  Google Scholar 

  • Wang K, Song XL, Han ZG, Guo W, Yu JZ, Sun J, Pan J, Kohel RJ, Zhang TZ (2006b) Complete assignment of the chromosomes of Gossypium hirsutum L.by translocation and fluorescence in situ hybridization mapping. Theor Appl Genet 113:73–80

    Article  PubMed  CAS  Google Scholar 

  • Wu ZB, He Q, Chen P (2002) Screen of resistance to Fusarium and Verticillium wilts of cotton. Acta Agrom Sin 28:713–717

    Google Scholar 

  • Zeng ZB (1994) Precision mapping of quantitative trait loci. Genetics 136:1457–1468

    PubMed  CAS  Google Scholar 

  • Zhang J, Guo WZ, Zhang TZ (2002) Molecular linkage map of allotetraploid cotton (Gossypium hirsutium L × Gossypium barbadense L) with a haploid population. Theor Appl Genet 105:1166–1174

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

This work financially supported in part by grants from the High-tech program 863 (2006AA100105), Jiangsu province key project (BE2008310), the 111 Project (B08025) and the Project of Xinjiang Bingtuan Doctoral Funding (2004) and the Key Project of Science and Technology in Xinjiang Uyghur Autonomy (200311101).

Author information

Authors and Affiliations

  1. National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, 210095, Nanjing, Jiangsu, China

    Peizheng Wang, Wangzhen Guo & Tianzhen Zhang

  2. Xinjiang Condy Agriculture Science and Technology Development Co. Ltd, Urumqi, 830011, China

    Li Su, Li Qin & Baomin Hu

Authors
  1. Peizheng Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Li Su
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Li Qin
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Baomin Hu
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Wangzhen Guo
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Tianzhen Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Tianzhen Zhang.

Additional information

Communicated by Y. Xue.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Wang, P., Su, L., Qin, L. et al. Identification and molecular mapping of a Fusarium wilt resistant gene in upland cotton. Theor Appl Genet 119, 733–739 (2009). https://doi.org/10.1007/s00122-009-1084-4

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00122-009-1084-4

Keywords

Search

Navigation