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Distribution of S haplotypes and its relationship with restorer–maintainers of self-incompatibility in cultivated Brassica napus

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Abstract

Brassica napus (AACC, 2n = 38) is a self-compatible amphidiploid plant that arose from the interspecies hybridization of two self-incompatible species, B. rapa (AA, 2n = 20) and B. oleracea (CC, 2n = 18). Self-incompatibility (S) haplotypes in one self-incompatible line and 124 cultivated B. napus lines were detected using S-locus-specific primers, and their relationships with restorer–maintainers were investigated. Two class I (S-I SLG a and S-I SLG b) and four class II (S-II SLG a, S-II SLG b, S-II SP11 a and S-II SP11 b) S haplotypes were observed, of which S-II SP11 b was newly identified. The nucleotide sequence of SP11 showed little similarity to the reported SP11 alleles. The lines were found to express a total of eleven S genotypes. The self-incompatible line had a specific genotype consisting of S-II SP11 a, similar to B. rapa S-60, and S-II SLG a, similar to B. oleracea S-15. Restorers expressed six genotypes: the most common genotype contained S-I SLG a, similar to B. rapa S-47, and S-II SLG b, similar to B. oleracea S-15. Maintainers expressed nine genotypes: the predominant genotype was homozygous for two S haplotypes, S-II SLG a and S-II SP11 b. One genotype was specific to restorers and four genotypes were specific to maintainers, whereas five genotypes were expressed in both restorers and maintainers. This suggests that there is no definitive correlation between the distribution of S genotypes and restorer–maintainers of self-incompatibility. The finding that restorers and maintainers express unique genotypes, and share some common genotypes, would be valuable for detecting the interaction of S haplotypes in inter- or intra-genomes as well as for developing markers-assisted selection in self-incompatibility hybrid breeding.

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Acknowledgments

This research was financed by funds from the National Key Basic Research Special Foundation of China (2007CB1090) and supported by the Program for Changjiang Scholar and Innovative Research Team in University (IRT0442).

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Authors and Affiliations

  1. National Key Laboratory of Crop Genetic Improvement, National Center of Rapeseed Improvement in Wuhan, Huazhong Agricultural University, Wuhan, 430070, People’s Republic of China

    Xingguo Zhang, Chaozhi Ma, Jiayou Tang, Wei Tang, Jinxing Tu, Jinxiong Shen & Tingdong Fu

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  1. Xingguo Zhang
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  2. Chaozhi Ma
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  3. Jiayou Tang
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  4. Wei Tang
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  7. Tingdong Fu
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Correspondence to Chaozhi Ma.

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Communicated by C. F. Quiros.

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122_2008_763_MOESM1_ESM.doc

Supplementary Table 1 List of plant material origins, the S phenotype of F1 generation plants, and the result of PCR amplification with S-locus specific primers in the B. napus lines. (DOC 331 kb)

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Zhang, X., Ma, C., Tang, J. et al. Distribution of S haplotypes and its relationship with restorer–maintainers of self-incompatibility in cultivated Brassica napus . Theor Appl Genet 117, 171–179 (2008). https://doi.org/10.1007/s00122-008-0763-x

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  • DOI: https://doi.org/10.1007/s00122-008-0763-x

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