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The introgression of chromosome 6P specifying for increased numbers of florets and kernels from Agropyron cristatum into wheat

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Abstract

A wheat (Triticum aestivum L.) line 4844 with superior numbers of florets and grains per spike was derived from the cross between Fukohokomugi wheat and Agropyron cristatum (L.) Gaertn. In order to determine the genetic control of floret and kernel number per spike in this line, chromosome addition and substitution lines that were derived from line 4844 were characterized by means of in situ hybridization, microsatellite (SSR), and gliadin analyses. Genomic in situ hybridization analysis with biotinylated P genomic DNA of A. cristatum as a probe demonstrated that the increased number of florets and grains in a spike was associated with the introgression of an A. cristatum chromosome. Fluorescence in situ hybridization, using a repetitive sequence, pAs1, derived from Aegilops squarrosa L., indicated the replacement of chromosome 6D of wheat in the wheat-A. cristatum chromosome substitution lines. This was confirmed by microsatellite analyses with wheat SSR markers specific for chromosome 6D, suggesting that the A. cristatum chromosome was homoeologous to group 6 and was therefore designated as 6P. This conclvsion was further confirmed by amplification using EST-SSR markers and gliadin analysis. The increased number of florets and kernels within a spike of the wheat-A. cristatum hybrids thus was controlled by gene(s) located on A. cristatum chromosome 6P.

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Acknowledgment

The financial supports provided by the Ministry of Science and Technology of China (project numbers 2006CB101700 and 2004BA525B03) are gratefully appreciated.

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Authors and Affiliations

  1. National Key Facilities for Crop Genetic Resources and Improvement (NFCRI), Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100081, People’s Republic of China

    Jun Wu, Xinmin Yang, Hongjie Li, Lihui Li, Xiuquan Li & Weihua Liu

  2. College of Agronomy, Northwest Sci-Tech University of Agriculture and Forestry, Yangling, 712100, Shanxi Province, People’s Republic of China

    Jun Wu & Hui Wang

Authors
  1. Jun Wu
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  2. Xinmin Yang
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  3. Hui Wang
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  4. Hongjie Li
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  5. Lihui Li
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  6. Xiuquan Li
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  7. Weihua Liu
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Correspondence to Lihui Li.

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Communicated by M. Morell.

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Wu, J., Yang, X., Wang, H. et al. The introgression of chromosome 6P specifying for increased numbers of florets and kernels from Agropyron cristatum into wheat. Theor Appl Genet 114, 13–20 (2006). https://doi.org/10.1007/s00122-006-0405-0

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  • DOI: https://doi.org/10.1007/s00122-006-0405-0

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