Abstract
Wheat yellow mosaic disease, which is caused by wheat yellow mosaic bymovirus (WYMV) and transmitted by soil-borne fungus, results in severe damage on wheat (Triticum aestivum L.) production in China. For development of resistant cultivars to reduce wheat yield losses due to wheat yellow mosaic disease, resistance test and genetic analysis indicated that a single dominant gene in wheat cultivar Yangfu 9311 contributed to the resistance. Bulk segregant analysis was used to identify microsatellite markers linked to the resistance gene in an F2 population derived from the cross Yangfu 9311 (resistant) × Yangmai 10 (susceptible). Microsatellite markers Xwmc41, Xwmc181, Xpsp3039, and Xgwm349 were co-dominantly or dominantly linked with the gene responsible for WYMV resistance at a distance of 8.1–11.6 cM. Based on the wheat microsatellite consensus map and the results from amplification of the cultivar Chinese Spring nulli-tetrasomic stocks, the resistance gene to wheat yellow mosaic disease derived from Yangfu 9311, temporarily named as YmYF, was thus mapped on the long arm of chromosome 2D (2DL).
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References
Autrique E, Singh RP, Tanksley SD, Sorrells ME (1995) Molecular markers for four leaf rust resistance genes introgressed into wheat from wild relatives. Genome 38:75–83
Bassam BJ, Caetano-Anolles G, Gresshoff PM (1991) Fast and sensitive silver staining of DNA in polyacrylamide gels. Anal Biochem 196:80–83
Chagué V, Fahima T, Dahan A, Sun GL, Korol AB, Ronin YI, Grama A, Röder MS, Nevo E (1999) Isolation of microsatellite and RAPD markers flanking the Yr15 gene of wheat using NILs and bulked segregant analysis. Genome 42:1050–1056
Chen J, Cheng Y, Chen JP (2000) Biological and molecular biological characterization of wheat yellow mosaic and wheat spindle streak mosaic bymoviruses. Virol Sin 15:97–105
Gupta PK, Balyan HS, Edwards KJ, Isaac P, Korzun V, Röder M, Gautier MF, Joudrier P, Schlatter AR, Dubcovsky J, De la Pena RC, Khairallah M, Penner G, Hayden MJ, Sharp P, Keller B, Wang RCC, Hardouin JP, Jack P, Leroy P (2002) Genetic mapping of 66 new microsatellite (SSR) loci in bread wheat. Theor Appl Genet 105:413–422
Hou QS (1993) Discussion of several issues on fugal-transmitted wheat virus diseases. Acta Phytopathol Sin 23:97–99
Inouye T (1969) Viral pathogen of the wheat yellow mosaic disease. Nogaku Kenkyu 53:61–68
Jefferies SP, King BJ, Barr AR, Warner P, Logue SJ, Langridge P (2003) Marker-assisted backcross introgression of the Yd2 gene conferring resistance to barley yellow dwarf virus in barley. Plant Breed 122:52–56
Khan AA, Bergstrom GC, Nelson JC, Sorrells ME (2000) Identification of RFLP markers for resistance to wheat spindle streak mosaic bymovirus (WSSMV) disease. Genome 43:477–482
Kosambi DD (1944) The estimation of map distance from recombination values. Ann Eugen 12:172–175
Lander ES, Green P, Abrahamson J, Barlow A, Daly MJ, Lincoln SE, Newburg L (1987) MAPMAKER: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1:174–181
Li DW, Han CG, Xing YM, Tian ZF, Yu JL, Cai ZN, Liu Y (1997) Identification of the wheat yellow mosaic virus occurring in China by RT-PCR. Acta Phytopathol Sin 27:303–307
Liu WH, He ZT, Geng B, Hou MS, Zhang M, Nie H, Han YP, Han CG, Wang JR, Yu JL, Chen XL (2004) Identification of resistance to yellow mosaic disease of wheat and analysis for its inheritance of some varieties. Acta Phytopathol Sin 34:542–547
Michelmore RW, Paran I, Kesseli RV (1991) Identification of markers linked to disease resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions using segregation populations. Proc Natl Acad Sci USA 88:9828–9832
Pestsova E, Ganal MW, Röder MS (2000) Isolation and mapping of microsatellite markers specific for the D genome of bread wheat. Genome 43:689–697
Qin JZ, Li ZM, Tao JF, Qin Y (1986) Primary study on resistance inheritance to yellow mosaic disease of wheat. J Sichuan Agric Univ 4:17–28
Robert O, Abelard C, Dedryver F (1999) Identification of molecular markers for the detection of the yellow rust resistance gene Yr17 in wheat. Mol Breed 5:167–175
Röder MS, Korzun V, Wendehake K, Plaschke J, Tixier M-H, Leroy P, Ganal MW (1998) A microsatellite map of wheat. Genetics 149:2007–2023
Saghai-Maroof MA, Soliman KM, Jorgensen RA, Allard RW (1984) Ribosomal DNA spacer length polymorphism in barley: mendelian inheritance, chromosomal location and population dynamics. Proc Natl Acad Sci USA 81:8014–8018
Shi ZX, Chen XM, Line RF, Leung H, Wellings CR (2001) Development of resistance gene analog polymorphism markers for the Yr9 gene resistance to wheat stripe rust. Genome 44:509–516
Somers DJ, Isaac P, Edwards K (2004) A high-density microsatellite consensus map for bread wheat (Triticum aestivum L.). Theor Appl Genet 109:1105–1114
Stoutjesdijk P, Kammholz SJ, Kleve S, Matsay S, Banks PM, Larkin PJ (2001) PCR-based molecular marker for the Bdv2 Thinopyrum intermedium source of barley yellow dwarf virus resistance in wheat. Aust J Agric Res 52:1383–1388
Sun GL, Fahima T, Korol AB, Turpeinen T, Grama A, Ronin YI, Nevo E (1997) Identification of molecular markers linked to the Yr15 stripe rust resistance gene of wheat originated in wild emmer wheat, Triticum dicoccoides. Theor Appl Genet 95:622–628
Talbert LE, Bruckner PL, Smith LY, Sears R, Martin TJ (1996) Development of PCR markers linked to resistance to wheat streak mosaic virus in wheat. Theor Appl Genet 93:463–467
Usugi T, Kashiwazaki S, Omura T, Tsuchizaki T (1989) Some properties of nucleic acids and coat protein of soil-borne filamentous viruses. Ann Phytopathol Soc Jpn 55:26–31
Yan GP, Chen XM, Line RF, Wellings CR (2003) Resistance gene-analog polymorphism markers co-segregating with the Yr5 gene for resistance to wheat stripe rust. Theor Appl Genet 106:636–643
Yao JB, Yang XM, Yao GC, Wang JN, Huang SD, Qian CM, Zhou CF (1999) Resistant inheritance and application strategies of wheat cultivar Ningmai 9 for streak mosaic disease. Tritical Crops 19:28–29
Yu JL, Yan LY, Su N, Huo ZJ, Li DW, Han CG, Yang LL, Cai ZN, Liu Y (1999) Analysis of nucleotide sequence of wheat yellow mosaic virus genomic RNAs. Sci China (Ser C) 42:554–560
Zhang ZY, Xin ZY, Ma YZ, Chen X, Xu QF, Lin ZS (1999) Mapping of a BYDV resistance gene from Thinopyrum intermedium in wheat background by molecular markers. Sci China (Ser C) 29:413–417
Zhou GH, Chen JB, Chen SM (1990) Comparative identification of wheat spindle streak mosaic virus from different plants in China. Acta Phytopathol Sin 20:107–110
Zhou YJ, Cheng ZB, Hou QS, Fan YJ, Wu SH (2000) Resistance of wheat varieties to wheat spindle streak mosaic disease. Acta Phytophyl Sin 27:102–106
Acknowledgements
This work is supported by National Natural Science Foundation of China (NSFC grant no. 30270874) and “Hi-Tech” Program (grant no.2001AA212111). The authors thank the John Innes Centre for kindly providing the unpublished primers.
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Liu, W., Nie, H., Wang, S. et al. Mapping a resistance gene in wheat cultivar Yangfu 9311 to yellow mosaic virus, using microsatellite markers. Theor Appl Genet 111, 651–657 (2005). https://doi.org/10.1007/s00122-005-2012-x
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DOI: https://doi.org/10.1007/s00122-005-2012-x