Abstract
The broad-spectrum stem rust resistance gene Sr2 has provided protection in wheat against Puccinia graminis Pers. f. sp. tritici for over 80 years. The Sr2 gene and an associated dark pigmentation trait, pseudo-black chaff (PBC), have previously been localized to the short arm of chromosome 3B. In a first step towards the positional-based cloning of Sr2, we constructed a high-resolution map of this region. The wheat EST (wEST) deletion bin mapping project provided tightly linked cDNA markers. The rice genome sequence was used to infer the putative gene order for orthologous wheat genes and provide additional markers once the syntenic interval in rice was identified. We used this approach to map six wESTs that were collinear with the physical order of the corresponding genes on rice chromosome 1 suggesting there are no major re-arrangements between wheat and rice in this region. We were unable to separate by recombination the tightly linked morphological trait, PBC from the stem rust resistance gene suggesting that either a single gene or two tightly linked genes control both traits.
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References
Ahn S, Anderson JA, Sorrells ME, Tanksley SD (1993) Homoeologous relationships of rice, wheat and maize chromosomes. Mol Gen Genet 241:483–490
Brueggeman R, Rostoks N, Kudrna D, Kilian A, Han F, Chen J, Druka A, Steffenson B, Kleinhofs A (2002) The barley stem rust-resistance gene Rpg1 is a novel disease-resistance gene with homology to receptor kinases. Proc Natl Acad Sci 99:9328–9333
Brunner S, Keller B, Feuillet C (2003) A large rearrangement involving genes and low copy DNA interrupts the microcolinearity between rice and barley at the Rph7 locus. Genetics 164:673–683
Devos KM (2005) Updating the ‘Crop Circle’. Curr Opinion Plant Biol 8:155–162
Guyot R, Yahiaoui N, Feuillet C, Keller B (2004) In silico comparative analysis reveals a mosaic conservation of genes within a novel colinear region in wheat chromosome 1AS and rice chromosome 5S. Func Integr Genomics 4:47–58
Hanson WD (1959) Minimum family sizes for the planning of genetic experiments. Agron J 51:711–715
Hare RA, McIntosh RA (1979) Genetic and cytogenetic studies of durable adult-plant resistances in “Hope” and related cultivars to wheat rusts. Z Pflanzenzuctg 83:350–367
Lagudah ES, Appels R, McNeil D (1991) The Nor-D3 locus of Triticum tauschii: natural variation and linkage to markers in chromosome 5. Genome 34:387–395
Liu S, Anderson JA (2003) Targeted molecular mapping of a major wheat QTL for Fusarium head blight resistance using wheat ESTs and synteny with rice. Genome 26:817–823
Lyngkjær MF, Newton AC, Atzema JL, Baker SJ (2000) The barley mlo-gene: an important powdery mildew resistance source. Agronomie 20:745–756
McIntosh RA (1988) The role of specific genes in breeding for durable stem rust resistance in wheat and triticale. In: Simmonds NW, Rajaram S (eds) Breeding strategies for resistance to the rusts of wheat. CIMMYT, Mexico, pp 1–9
McIntosh RA, Park RF, Wellings CR (1995) Wheat rusts: an atlas of resistance genes. CSIRO Publications, East Melbourne
Moore G, Devos KM, Wang Z, Gale MD (1995) Grasses, line up and form a circle. Curr Biol 5:737–739
Munkvold JD, Greene RA, Bermundez-Kandianis CE, La Rota CM, Edwards H et al (2004) Group 3 chromosome bin maps of wheat and their relationship to rice chromosome. Genetics 168:639–650
Qi LL, Echalier Chao BS, Lazo GR et al. (2004) A chromosome bin map of 16,000 expressed sequence tag loci and distribution of genes among the three genomes of polyploid wheat. Genetics 168:701–712
Rajaram S, Singh RP, Torres E (1988) Current CIMMYT approaches in breeding for rust resistance. In: Simmonds NW, Rajaram S (eds) Breeding strategies for resistance to the rusts of wheat. CIMMYT, Mexico, pp 101–118
Roelfs AP (1988) Resistance to leaf and stem rusts in wheat. In: Simmonds NW, Rajaram S (eds) Breeding strategies for resistance to the rusts of wheat. CIMMYT, Mexico, pp 10–22
Seah S, Sivasithamparam K, Karakousis A, Lagudah ES (1998) Cloning and characterization of a family of disease resistance gene analogs from wheat and barley. Theor Appl Genet 97:937–945
Sheen S-J, Ebeltoft DC, Smith GS (1968) Association and inheritance of “black chaff” and stem rust reactions in Conley wheat crosses. Crop Sci 8:477–480
Singh RP (1992) Genetic association of leaf rust resistance gene Lr34 with adult plant resistance to stripe rust in bread wheat. Phytopathology 82:835–838
Sorrells ME, La Rota M, Bermudez-Kandianis CE et al. (2003) Comparative DNA sequence analysis of wheat and rice genomes. Genome Res 13:1818–1827
Spielmeyer W, Sharp PJ, Lagudah ES (2003) Identification and validation of markers linked to broad-spectrum stem rust resistance gene Sr2 in wheat (Triticum aestivum L.). Crop Sci 43:333–336
Yan L, Loukoianov A, Tranquilli G, Helguera M, Fahima T, Dubcovsky J (2003) Positional cloning of the wheat vernalization gene VRN1. Proc Natl Acad Sci USA 100:6263–6268
Acknowledgements
This work was funded by Graingene, a joint venture between AWB Limited, CSIRO, GRDC and Syngenta Seeds. Technical assistance provided by Sutha Chandramohan is gratefully acknowledged.
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Kota, R., Spielmeyer, W., McIntosh, R. et al. Fine genetic mapping fails to dissociate durable stem rust resistance gene Sr2 from pseudo-black chaff in common wheat (Triticum aestivum L.). Theor Appl Genet 112, 492–499 (2006). https://doi.org/10.1007/s00122-005-0151-8
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DOI: https://doi.org/10.1007/s00122-005-0151-8