Skip to main content
SpringerLink
Log in

Microsatellite mapping of a Triticum urartu Tum. derived powdery mildew resistance gene transferred to common wheat (Triticum aestivum L.)

  • Original Paper
  • Published:
Theoretical and Applied Genetics Aims and scope Submit manuscript

Abstract

A powdery mildew resistance gene from Triticum urartu Tum. accession UR206 was successfully transferred into hexaploid wheat (Triticum aestivum L.) through crossing and backcrossing. The F1 plants, which had 28 chromosomes and an average of 5.32 bivalents and 17.36 univalents in meiotic pollen mother cells (PMC), were obtained through embryos rescued owing to shriveling of endosperm in hybrid seed of cross Chinese Spring (CS) × UR206. Hybrid seeds were produced through backcrossing F1 with common wheat parents. The derivative lines had normal chromosome numbers and powdery mildew resistance similar to the donor UR206, indicating that the powdery mildew resistance gene originating from T. urartu accession UR206 was successfully transferred and expressed in a hexaploid wheat background. Genetic analysis indicated that a single dominant gene controlled the powdery mildew resistance at the seedling stage. To map and tag the powdery mildew resistance gene, 143 F2 individuals derived from a cross UR206 × UR203 were used to construct a linkage map. The resistant gene was mapped on the chromosome 7AL based on the mapped microsatellite makers. The map spanned 52.1 cM and the order of these microsatellite loci agreed well with the established microsatellite map of chromosome arm 7AL. The resistance gene was flanked by the microsatellite loci Xwmc273 and Xpsp3003, with the genetic distances of 2.2 cM and 3.8 cM, respectively. On the basis of the origin and chromosomal location of the gene, it was temporarily designated PmU.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  • Bougot Y, Lemoine J, Pavoine MT, Barloy D, Doussinault G (2002) Identification of a microsatellite marker associated with Pm3 resistance alleles to powdery mildew in wheat. Plant Breed 121:325–329

    Article  CAS  Google Scholar 

  • Bryan GJ, Collins AJ, Stephenson P, Orry A, Smith JB, Gale MD (1997) Isolation and characterization of microsatellites from hexaploid bread wheat. Theor Appl Genet 94:557–563

    Article  CAS  Google Scholar 

  • Cenci A, D’Ovidio R, Tanzarella OA, Ceoloni C, Porceddu E (1999) Identification of molecular markers linked to Pm13, an Aegilops longissima gene conferring resistance to powdery mildew in wheat. Theor Appl Genet 98:448–454

    Article  CAS  Google Scholar 

  • Chapman V, Miller TE, Riley R (1976) Equivalence of the A genome of bread wheat and that of Triticum urartu. Genet Res 27:69–76

    Article  Google Scholar 

  • Donini P, Koebner RMD, Ceoloni C (1995) Cytogenetic and molecular mapping of the wheat- Aegilops longissima chromatin breakpoints in powdery mildew resistant introgression lines. Theor Appl Genet 91:738–743

    Article  CAS  Google Scholar 

  • Dvořák J, McGuire PE, Cassidy B (1988) Apparent sources of the A genome of wheat inferred from polymorphism in abundance and restriction fragment length of repeated nucleotide sequences. Genome 30:680–689

    Google Scholar 

  • Dvořák J, di Terlizzi P, Zhang HB, Resta P (1993) The evolution of polyploidy wheat: identification of the A genome donor species. Genome 36:21–31

    Article  PubMed  Google Scholar 

  • Dyck PL, Keber ER (1970) Inheritance in hexaploid wheat of adult plant leaf rust resistance derived from Aegilops squarrosa. Can J Genet Cytol 12:175–180

    Google Scholar 

  • Fahima T, Röder MS, Grama A, Nevo E (1998) Microsatellite DNA polymorphism divergence in Triticum dicoccoides accessions highly resistant to yellow rust. Theor Appl Genet 96:187–195

    Article  CAS  Google Scholar 

  • Feldman M, Sears ER (1981) The wild gene resources of wheat. Sci Am 244:102–112

    Article  Google Scholar 

  • Gupta PK, Balyan HS, Edwards KJ, Isaac P, Korzun V, Röder M, Gautier MF, Joudrier P, Schlatter AR, Dubcovscy J, De la Pena RC, Khairallah M, Penner G, Hayden MJ, Sharp P, Keller B, Wang RCC, Hardouin JP, Jack P, Leroy P (2002) Genetic mapping of 66 new microsatellite (SSR) loci in bread wheat. Theor Appl Genet 105:413–422

    Article  PubMed  CAS  Google Scholar 

  • Hartl L, Weiss H, Zeller FJ, Jahoor A (1993) Use of RFLP markers for the identification of alleles of the Pm3 locus conferring powdery mildew resistance in wheat (Triticum aestivum L.). Theor Appl Genet 86:959–963

    Article  CAS  Google Scholar 

  • Hartl L, Weiss H, Stephan U, Zeller FJ, Jahoor A (1995) Molecular identification of powdery mildew resistance genes in common wheat (Triticum aestivum L.). Theor Appl Genet 90:601–606

    Article  Google Scholar 

  • Hartl L, Mohler V, Zeller FJ, Hsam SLK, Schweizer G (1999) Identification of AFLP markers closely linked to the powdery mildew resistance genes Pm1c and Pm4a in common wheat. Genome 42:322–329

    Article  CAS  Google Scholar 

  • Hsam SLK, Huang XQ, Ernst F, Hartl L, Zeller FJ (1998) Chromosomal location of genes for resistance to powdery mildew in common wheat (Triticum aestivum L. em. Thell.). 5. Alleles at the Pm1 locus. Theor Appl Genet 96:1129–1134

    Article  CAS  Google Scholar 

  • Hsam SLK, Mohler V, Hartl L, Wenzel G, Zeller FJ (2000) Mapping of powdery mildew and leaf rust resistance genes on the wheat-rye translocated chromosome T1BL.1RS using molecular and biochemical markers. Plant Breed 119:87–89

    Article  CAS  Google Scholar 

  • Hsam SLK, Lapochkina IF, Zeller FJ (2003) Chromosomal location of genes for resistance to powdery mildew in common wheat (Triticum aestivum L. em Thell.). 8. Gene Pm32 in a wheat-Aegilops speltoides translocation line. Euphytica 133:367–370

    Article  Google Scholar 

  • Hu XY, Ohm HW, Dweikat I (1997) Identification of RAPD markers linked to the gene Pm1 for resistance to powdery mildew in wheat. Theor Appl Genet 94:832–840

    Article  CAS  Google Scholar 

  • Huang XQ, Hsam SLK, Zeller FJ (1997) Identification of powdery mildew resistance genes in common wheat (Triticum aestivum L.). Cultivars, landraces and breeding lines grown in China. Plant Breed 116:233–238

    Article  Google Scholar 

  • Huang XQ, Hsam SLK, Zeller FJ, Wenzel G, Mohler V (2000) Molecular mapping of the wheat powdery mildew resistance gene Pm24 and marker validation for molecular breeding. Theor Appl Genet 101:407–414

    Article  CAS  Google Scholar 

  • Huang XQ, Wang LX, Xu MX, Röder MS (2003) Microsatellite mapping of the powdery mildew resistance gene Pm5e in common wheat (Triticum aestivum L.). Theor Appl Genet 106:858–865

    PubMed  CAS  Google Scholar 

  • Järve K, Peusha HO, Tsymbalova J, Tamm S, Devos KM, Enno TM (2000) Chromosomal location of a Triticum timopheevi–derived powdery mildew resistance gene transferred to common wheat. Genome 43:377–381

    Article  PubMed  Google Scholar 

  • Jia J, Devos KM, Chao S, Miller TE, Reader SM, Gale MD (1996) RFLP-based maps of the homoeologous group-6 chromosomes of wheat and their application in the tagging of Pm12, a powdery mildew resistance gene transferred from Aegilops speltoides to wheat. Theor Appl Genet 92:559–565

    Article  CAS  Google Scholar 

  • Kerber ER, Dyck PL (1973) Inheritance of stem rust resistance transferred from diploid wheat and chromosome location of the gene involved. Can J Genet Cytol 15:397–409

    Google Scholar 

  • Kosambi DD (1944) The estimation of map distances from recombination values. Ann Eugen 12:172–175

    Google Scholar 

  • Lincoln S, Daly M, Lander E (1992) Construction genetic maps with MAPMAKER/EXP3.0. Whitehead Institute Techn Rep 3rd edn. Whitehead Institute, Cambridge, Masachussetts, USA

    Google Scholar 

  • Liu ZY, Sun QX, Ni ZF, Yang TM (1999) Development of SCAR markers linked to the Pm21 gene conferring resistance to powdery mildew in common wheat. Plant Breed 118:215–219

    Article  CAS  Google Scholar 

  • Liu ZY, Sun QX, Ni ZF, Nevo E, Yang TM (2002) Molecular characterization of a novel powdery mildew resistance gene Pm30 in wheat originating from wild emmer. Euphytica 123:21–29

    Article  CAS  Google Scholar 

  • Ma H, Singh RP, Mujeeb-Kazi A (1995) Suppression/expression of resistance to stripe rust in synthetic hexaploid wheat (Triticum turgidum × T. tauschii). Euphytica 83:87–93

    Article  Google Scholar 

  • Ma H, Singh RP, Mujeeb-Kazi A (1997) Resistance to stripe rust in durum wheats, A-genome diploids, and their amphiploids. Euphytica 94:279–286

    Article  Google Scholar 

  • Ma ZQ, Sorrells ME, Tanksley SD (1994) RFLP markers linked to powdery mildew resistance genes Pm1, Pm2, Pm3 and Pm4 in wheat. Genome 37:871–875

    CAS  PubMed  Google Scholar 

  • Ma ZQ, Wei JB, Cheng SH (2004) PCR-based markers for the powdery mildew resistance gene Pm4a in wheat. Theor Appl Genet 109:140–145

    Article  PubMed  CAS  Google Scholar 

  • McIntosh RA, Hart GE, Devos KM, Gale MD, Rogers WJ (1998) Catalogue of gene symbols for wheat. http://wheat.pw.usda.gov/ggpages/wgc/98

  • McIntosh RA, Devos KM, Dubcovsky J, Morris CF, Rogers WJ (2003) Catalogue of gene symbols for wheat: 2003 supplement. http://wheat.pw.usda.gov/ggpages/wgc/2003upd.html

  • Michelmore RW, Paran I, Kesseli RV (1991) Identification of markers linked to disease-resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions by using segregating populations. Proc Natl Acad Sci USA 88:9828–9832

    Article  PubMed  CAS  Google Scholar 

  • Neu C, Stein N, Keller B (2002) Genetic mapping of the Lr20-Pm1 resistance locus reveals suppressed recombination on chromosome arm 7AL in hexaploid wheat. Genome 45:737–744

    Article  PubMed  CAS  Google Scholar 

  • Paull JG, Pallotta MA, Langridge P, The TT (1994) RFLP markers associated with Sr22 and recombination between chromosome 7A of bread wheat and the diploid species Triticum boeoticum. Theor Appl Genet 89:1039–1045

    Article  CAS  Google Scholar 

  • Plaschke J, Ganal MW, Röder MS (1995) Detection of genetic diversity in closely related bread wheat using microsatellite markers. Theor Appl Genet 91:1001–1007

    Article  CAS  Google Scholar 

  • Qi LL, Cao MS, Chen PD, Li WL, Liu DJ (1996) Identification, mapping, and application of polymorphic DNA associated with resistance gene Pm21 of wheat. Genome 39:191–197

    CAS  PubMed  Google Scholar 

  • Röder MS, Plaschke J, König SU, Börner A, Sorrells ME, Tanksley SD, Ganal MW (1995) Abundance, variability and chromosomal location of microsatellites in wheat. Mol Gen Genet 246:327–333

    Article  PubMed  Google Scholar 

  • Röder MS, Korzun V, Wendehake K, Plaschke J, Tixier MH, Leroy P, Ganal MW (1998) A microsatellite map of wheat. Genetics 149:2007–2023

    PubMed  Google Scholar 

  • Rong JK, Millet E, Manisterski J, Feldman M (2000) A new powdery mildew resistance gene: Introgression from wild emmer into common wheat and RFLP-based mapping. Euphytica 115:121–126

    Article  CAS  Google Scholar 

  • Schneider DM, Heun M, Fischbeek G (1991) Inheritance of the powdery mildew resistance gene Pm9 in relation to Pm1 and Pm2 of wheat. Plant Breed 107:161–164

    Article  Google Scholar 

  • Sears ER, Briggle LW (1969) Mapping the gene Pm1 for resistance to Erysiphe graminis f. sp . tritici on chromosome 7A of wheat. Crop Sci 9:96–97

    Article  Google Scholar 

  • Sharp PJ, Kreis M, Shewry PR, Gale MD (1988) Location of β-amylase sequence in wheat and its relatives. Theor Appl Genet 75:289–290

    Article  Google Scholar 

  • Shi AN, Leath S, Murphy JP (1998) A major gene for powdery mildew resistance transferred to common wheat from wild einkorn wheat. Phytopathology 88:144–147

    Article  CAS  PubMed  Google Scholar 

  • Singrün Ch, Hsam SL, Hartl L, Zeller FJ, Mohler V (2003) Powdery mildew resistance gene Pm22 in cultivar Virest is a member of the complex Pm1 locus in common wheat (Triticum aestivum L. em Thell.). Theor Appl Genet 106:1420–1424

    PubMed  Google Scholar 

  • Singrün Ch, Hsam SL, Zeller FJ, Wenzel G, Mohler V (2004) Localization of a novel recessive powdery mildew resistance gene from common wheat line RD30 in the terminal region of chromosome 7AL. Theor Appl Genet 109:210–214

    Article  PubMed  CAS  Google Scholar 

  • Sourdille P, Singh S, Cadalen T, Brown-Guedira GL, Gay G, Qi LL, Gill BS, Dufour P, Murigneux A, Bernard M (2004) Microsatellite-based deletion bin system for the establishment of genetic-physical map relationships in wheat (Triticum aestivum L.). Funct Integr Genom 4:12–25

    Article  PubMed  CAS  Google Scholar 

  • Stakman EC, Stewart DM, Loegering WQ (1962) Identification of physiological races of Puccinia graminis var. tritici. US Dept Agric ARS E 617:1–53

    Google Scholar 

  • Stephenson P, Bryan G, Kirby J, Collins A, Devos K, Busso C, Gale M (1998) Fifty new microsatellite loci for the wheat genetic map. Theor Appl Genet 97:946–949

    Article  CAS  Google Scholar 

  • Tao W, Liu D, Liu J, Feng Y, Chen P (2000) Genetic mapping of the powdery mildew resistance gene Pm6 in wheat by RFLP analysis. Theor Appl Genet 100:564–568

    Article  CAS  Google Scholar 

  • Villareal RL, Singh RP, Mujeeb-Kazi A (1992) Expression of resistance to Puccinia recondite f.sp. tritici in synthetic hexaploid wheat. Vortr. Pflanzenzüchtg 24:253–255

    Google Scholar 

  • Xie CJ, Sun QX, Ni ZF, Yang ZM, Nevo E, Fahima T (2003) Chromosomal location of a Triticum dicoccoides-derived powdery mildew resistance gene in common wheat by using microsatellite markers. Theor Appl Genet 106:341–345

    PubMed  CAS  Google Scholar 

  • Yahiaoui N, Srichumpa P, Dudler R, Keller B (2004) Genome analysis at different ploidy levels allows cloning of the powdery mildew resistance gene Pm3b from hexaploid wheat. Plant J 34:528–538

    Article  CAS  Google Scholar 

  • Zeller FJ, Kong L, Hartl L, Mohler V, Hsam SLK (2002) Chromosomal location of genes for resistance to powdery mildew in common wheat (Triticum aestivum L. em Thell.). 7. Gene Pm29 in line Pova. Euphytica 123:187–194

    Article  CAS  Google Scholar 

  • Zhu ZD, Zhou RH, Kong XY, Dong YC, Jia JZ (2005) Microsatellite markers linked to two genes conferring resistance to powdery mildew in common wheat introgressed from Triticum carthlicum acc. PS5. Genome 48:585–590

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgements

This research was supported by the National Basic Research Program of China (973) (Grant No.2004CB117200). The authors would like to thank Dr. Steven S. Xu from USDA-ARS, Northern Crop Science Laboratory, for his critical reading of the manuscript. We also thank Mr. Reader from John Innes Centre, Norwich, UK, for kindly providing the Triticum urartu seeds.

Author information

Authors and Affiliations

  1. Key Laboratory of Crop Germplasm and Biotechnology, Ministry of Agriculture, Institute of Crop Germplasm Resources, Chinese Academy of Agricultural Sciences, 100081, Beijing, People’s Republic of China

    Y. C. Qiu, R. H. Zhou, X. Y. Kong & J. Z. Jia

  2. Key Laboratory of Northern Crop Disease Immunity, Ministry of Agriculture, College of Plant Protection, Shenyang Agricultural University, 110161, Shenyang, People’s Republic of China

    Y. C. Qiu & S. S. Zhang

Authors
  1. Y. C. Qiu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. R. H. Zhou
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. X. Y. Kong
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. S. S. Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. J. Z. Jia
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to J. Z. Jia.

Additional information

Communicated by B. Keller

Rights and permissions

Reprints and permissions

About this article

Cite this article

Qiu, Y.C., Zhou, R.H., Kong, X.Y. et al. Microsatellite mapping of a Triticum urartu Tum. derived powdery mildew resistance gene transferred to common wheat (Triticum aestivum L.). Theor Appl Genet 111, 1524–1531 (2005). https://doi.org/10.1007/s00122-005-0081-5

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00122-005-0081-5

Keywords

Search

Navigation