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QTL analyses of drought tolerance and growth for a Salix dasyclados × Salix viminalis hybrid in contrasting water regimes

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Abstract

Quantitative trait loci (QTL) for growth traits and water-use efficiency have been identified in two water regimes (normal and drought-treated) and for a treatment index. A tetraploid hybrid F2 population originating from a cross between a Salix dasyclados clone (SW901290) and a Salix viminalis clone (‘Jorunn’) was used in the study. The growth response of each individual including both above and below ground dry-matter production (i.e. shoot length, shoot diameter, aboveground and root dry weight, internode length, root dry weight/total dry weight, relative growth rate and leaf nitrogen content) was analysed in a replicated block experiment with two water treatments. A composite interval mapping approach was used to estimate number of QTL, the magnitude of the QTL and their position on genetic linkage maps. QTL specific for each treatment and for the treatment index were found, but QTL common across the treatments and the treatment index were also detected. Each QTL explained from 8% to 29% of the phenotypic variation, depending on trait and treatment. Clusters of QTL for different traits were mapped close to each other at several linkage groups, indicating either a common genetic base or tightly linked QTL. Common QTL identified between treatments and treatment index in the complex trait dry weight can be useful tools in the breeding and selection for drought stress tolerance in Salix.

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Acknowledgements

Technical assistance in the greenhouse with water treatments and harvest of plants by Eva-Marie Fryk, Barbro Johansson, Kristin-Sophie Mellsjö, Urban Pettersson, Christina Segerqvist and Ewa Winkler is highly appreciated. This study was supported by a grant from the Swedish National Energy Administration (project no. P13028-1).

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  1. Department of Plant Biology and Forest Genetics, Swedish University of Agricultural Sciences, 7080, 750 07, Uppsala, Sweden

    A. C. Rönnberg-Wästljung

  2. The Forestry Research Institute of Sweden, Uppsala Science Park, 751 83, Uppsala, Sweden

    C. Glynn

  3. Department of Short Rotation Forestry, Swedish University of Agricultural Sciences, 7016, 750 07, Uppsala, Sweden

    M. Weih

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  1. A. C. Rönnberg-Wästljung
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  3. M. Weih
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Correspondence to A. C. Rönnberg-Wästljung.

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D.B. Neale

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Rönnberg-Wästljung, A.C., Glynn, C. & Weih, M. QTL analyses of drought tolerance and growth for a Salix dasyclados × Salix viminalis hybrid in contrasting water regimes. Theor Appl Genet 110, 537–549 (2005). https://doi.org/10.1007/s00122-004-1866-7

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