Skip to main content
SpringerLink
Log in

Geographical patterns of genetic variation in the world collections of wild annual Cicer characterized by amplified fragment length polymorphisms

  • Original Paper
  • Published:
Theoretical and Applied Genetics Aims and scope Submit manuscript

Abstract

Cicer reticulatum, C. echinospermum, C. bijugum, C. judaicum, C. pinnatifidum, C. cuneatum and C. yamashitae are wild annual Cicer species and potential donors of valuable traits to improve chickpea (C. arietinum). As part of a large project to characterize and evaluate wild annual Cicer collections held in the world gene banks, AFLP markers were used to study genetic variation in these species. The main aim of this study was to characterize geographical patterns of genetic variation in wild annual Cicer germplasm. Phylogenetic analysis of 146 wild annual Cicer accessions (including two accessions in the perennial C. anatolicum and six cultivars of chickpea) revealed four distinct groups corresponding well to primary, secondary and tertiary gene pools of chickpea. Some possible misidentified or mislabelled accessions were identified, and ILWC 242 is proposed as a hybrid between C. reticulatum and C. echinospermum. The extent of genetic diversity varied considerably and was unbalanced between species with greatest genetic diversity found in C. judaicum. For the first time geographic patterns of genetic variation in C. reticulatum, C. echinospermum, C. bijugum, C. judaicum and C. pinnatifidum were established using AFLP markers. Based on the current collections the maximum genetic diversity of C. reticulatum, C. echinospermum, C. bijugum and C. pinnatifidum was found in southeastern Turkey, while Palestine was the centre of maximum genetic variation for C. judaicum. This information provides a solid basis for the design of future collections and in situ conservation programs for wild annual Cicer.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  • Abbo S, Berger J, Turner NC (2003) Evolution of cultivated chickpea: four bottlenecks limit diversity and constrain adaptation. Funct Plant Biol 30:1081–1087

    Google Scholar 

  • Benham JJ (2001) Genographer version 1.6, Boston. Available via http://hordeum.oscs.montana.edu/genographer

  • Berger J, Abbo S, Turner NC (2003) Ecogeography of annual Cicer species: the poor state of the world collection. Crop Sci 43:1076–1090

    Google Scholar 

  • Capo-chichi LJA, Weaver DB, Morton CM (2001) AFLP assessment of genetic variability among velvetbean (Mucuna sp.) accessions. Theor Appl Genet 103:1180–1188

    Article  CAS  Google Scholar 

  • Choumane W, Winter P, Weigand F, Kahl G (2000) Conservation and variability of sequence-tagged microsatellite sites (STMSs) from chickpea (Cicer arietinum L.) within the genus Cicer. Theor Appl Genet 101:269–278

    Article  CAS  Google Scholar 

  • Croser JS, Ahmad F, Clarke HJ, Siddique KHM (2003) Utilisation of wild Cicer in chickpea improvement—progress, constraints, and prospects. Aust J Agr Res 54:429–444

    Article  Google Scholar 

  • Heslop-Harrison JS (2002) Exploiting novel germplasm. Aust J Agr Res 53:873–879

    Article  Google Scholar 

  • Iruela M, Rubio J, Cubero JI, Gil J, Millan T (2002) Phylogenetic analysis in the genus Cicer and cultivated chickpea using RAPD and ISSR markers. Theor Appl Genet 104:643–651

    Article  CAS  PubMed  Google Scholar 

  • Kazan K, Muehlbauer FJ (1991) Allozyme variation and phylogeny in annual species of Cicer (Leguminosae). Plant Syst Evol 175:11–22

    CAS  Google Scholar 

  • Muehlbauer FJ, Kaiser WJ, Simon CJ (1994) Potential for wild species in cool season food legume breeding. Euphytica 73:109–114

    Article  Google Scholar 

  • Nei M (1987) Molecular evolutionary genetics. Columbia University Press, New York

    Google Scholar 

  • Nei M, Li WH (1979) Mathematical model for studying genetic variation in terms of restriction endonucleases. Proc Natl Acad Sci USA 76:5269–5273

    CAS  PubMed  Google Scholar 

  • Nguyen TT, Taylor PWJ, Redden RJ, Ford R (2004) Genetic diversity estimates in Cicer using AFLP analysis. Plant Breed 123:173–179

    Article  CAS  Google Scholar 

  • Sawkins MC, Maass BL, Pengelly BC, Newbury HJ, Ford-Lloyd BV, Maxted N, Smith R (2001) Geographical patterns of genetic variation in two species of Stylosanthes Sw. using amplified fragment length polymorphism. Mol Ecol 10:1947–1958

    Article  CAS  PubMed  Google Scholar 

  • Serret MD, Udupa SM, Weigand F (1997) Assessment of genetic diversity of cultivated chickpea using microsatellite-derived RFLP markers: implications for origin. Plant Breed 116:573–578

    CAS  Google Scholar 

  • Shan F, Clarke HJ, Yan G, Plummer JA, Siddique KHM (2004) Development of DNA fingerprinting keys for discrimination of Cicer echinospermum (P.H. Davis) accessions using AFLP markers. Aust J Agr Res 55:947–953

    Article  CAS  Google Scholar 

  • Shi GR (1993) Multivariate data analysis in palaeoecology and palaeobiogeography—a review. Palaeogeogr Palaeocl 105:199–234

    Article  Google Scholar 

  • Singh KB, Ocampo B (1993) Interspecific hybridization in annual Cicer species. J Genet Breed 47:199–204

    Google Scholar 

  • Singh KB, Ocampo B (1997) Exploitation of wild Cicer species for yield improvement in chickpea. Theor Appl Genet 95:418–423

    Article  Google Scholar 

  • Singh KB, Malhotra RS, Halila MH, Knights EJ, Verma MM (1994) Current status and future strategy in breeding chickpea for resistance to biotic and abiotic stresses. Euphytica 73:137–149

    Article  Google Scholar 

  • Singh KB, Ocampo B, Robertson LD (1998) Diversity for abiotic and biotic stress resistance in the wild annual Cicer species. Genet Resour Crop Evol 45:9–17

    Article  Google Scholar 

  • Stamigna C, Crino P, Saccardo F (2000) Wild relatives of chickpea: multiple disease resistance and problems to introgression in the cultigen. J Genet Breed 54:213–219

    Google Scholar 

  • Sudupak MA (2004) Inter and intra-species inter simple sequence repeat (ISSR) variation in the genus Cicer. Euphytica 135:229–238

    Article  CAS  Google Scholar 

  • Sudupak MA, Akkaya MS, Kence A (2004) Genetic relationships among perennial and annual Cicer species growing in Turkey assessed by AFLP fingerprinting. Theor Appl Genet 108:937–944

    Article  CAS  PubMed  Google Scholar 

  • Swofford DL (2002) PAUP: phylogenetic analysis using parsimony (and other methods), version 4.0b10, Sinaur, Sunderland

    Google Scholar 

  • van der Maesen LJG (1987) Origin, history and taxonomy of chickpea. In: Saxena MC, Singh KB (eds) The chickpea. CABI, UK, pp 11–34

    Google Scholar 

  • Virk PS, Newbury HJ, Jackson MT, Ford-Lloyd BV (1995) The identification of duplicate accessions within a rice germplasm collection using RAPD analysis. Theor Appl Genet 90:1049–1055

    Article  CAS  Google Scholar 

  • Zeid M, Schon C-C, Link W (2003) Genetic diversity in recent elite faba bean lines using AFLP markers. Theor Appl Genet 107:1304–1314

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgements

Fucheng Shan is grateful to the Grains Research and Development Corporation of Australia (GRDC) for a Postdoctoral Fellowship. We thank Mr. Ted Knights, Dr. Jens Berger, Dr. James Ridsdill-Smith, Dr. Tanveer Khan, Tamworth Centre for Crop Improvement Australia, ICARDA, ICRISAT and USDA for providing germplasm. Molecular analysis for this study was conducted using facilities at the State Agricultural Biotechnology Centre, Murdoch University, Western Australia.

Author information

Authors and Affiliations

  1. Centre for Legumes in Mediterranean Agriculture, Faculty of Natural and Agricultural Sciences, The University of Western Australia, 35 Stirling Hwy, Crawley, WA, 6009, Australia

    F Shan, HC Clarke, JA Plummer, G Yan & KHM Siddique

  2. School of Plant Biology, Faculty of Natural and Agricultural Sciences, The University of Western Australia, 35 Stirling Hwy, Crawley, WA, 6009, Australia

    JA Plummer & G Yan

Authors
  1. F Shan
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. HC Clarke
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. JA Plummer
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. G Yan
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. KHM Siddique
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to F Shan.

Additional information

Communicated by P. Langridge

Rights and permissions

Reprints and permissions

About this article

Cite this article

Shan, F., Clarke, H., Plummer, J. et al. Geographical patterns of genetic variation in the world collections of wild annual Cicer characterized by amplified fragment length polymorphisms. Theor Appl Genet 110, 381–391 (2005). https://doi.org/10.1007/s00122-004-1849-8

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00122-004-1849-8

Keywords

Search

Navigation