Skip to main content
SpringerLink
Log in

Identification of markers tightly linked to sbm recessive genes for resistance to Pea seed-borne mosaic virus

  • Original Paper
  • Published:
Theoretical and Applied Genetics Aims and scope Submit manuscript

Abstract

Two virus resistance loci on linkage groups II and VI have provided the only sources of natural resistance against Pea seed-borne mosaic virus (PSbMV, Potyviridae) in the important crop plant Pisum sativum L. A combination of parallel approaches was used to collate linked markers, particularly for sbm-1 resistance on linkage group VI. We have identified sequences derived from the genes for the eukaryotic translation initiation factors eIF4E and eIF(iso)4E as being very tightly linked to the resistance gene clusters on linkage groups VI and II, respectively. In particular, no recombinants between sbm-1 and eIF4E were found amongst 500 individuals of an F2 cross between the BC4 resistant line (JI1405) and its recurrent susceptible parent ‘Scout’. In a different mapping population, the gene eIF(iso)4E was also shown to be linked to sbm-2 on linkage group II. A parallel cDNA-AFLP comparison of pairs of resistant and susceptible lines also identified an expressed tag marker just 0.7 cM from sbm-1. eIF4E and eIF(iso)4E have been associated with resistance to related viruses in other hosts. This correlation strengthens the use of our markers as valuable tools to assist in breeding multiple virus resistances into peas, and identifies potential targets for resistance gene identification in pea.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2a, b
Fig. 3a, b
Fig. 4a, b
Fig. 5

Similar content being viewed by others

References

  • Bachem CW, van der Hoeven RS, de Bruijn SM, Vreugdenhil D, Zabeau M, Visser RG (1996) Visualization of differential gene expression using a novel method of RNA fingerprinting based on AFLP: analysis of gene expression during potato tuber development. Plant J 9:745–753

    CAS  PubMed  Google Scholar 

  • Borgstrom B, Johansen IE (2001) Mutations in Pea seed-borne mosaic potyvirus genome-linked protein VPg alter pathotype specific virulence in Pisum sativum. Mol Plant Microbe Interact 14:707–714

    CAS  PubMed  Google Scholar 

  • Bruun-Rasmussen M, Moller IS, Cordea M, Sundelin T, Johansen IE (2003) Molecular resistance determinants of the Bean yellow mosaic virus in Pisum sativum. EMBO Workshop – Genomic approaches in plant virology. Abstract, p 6

  • Christou P, Ford TL, Kofron M (1991) Production of transgenic rice (Oryza sativa L.) plants from agronomically important indica and japonica varieties via electric discharge particle acceleration of exogenous DNA into immature zygotic embryos. Bio technology 9:957–962

    Google Scholar 

  • Deom CM, Murphy JF, Paguio OR (1997) Resistance to Tobacco etch virus in Capsicum annum: inhibition of virus RNA accumulation. Mol Plant Microbe Interact 10:917-921

    CAS  Google Scholar 

  • Duprat A, Caranta C, Revers F, Menand B, Browning KS, Robaglia C (2002) The Arabidopsis eukaryotic initiation factor (iso)4E is dispensable for plant growth but required for susceptibility to potyviruses. Plant J 32:927–934

    Article  CAS  PubMed  Google Scholar 

  • Ellis THN (1994) Approaches to the genetic mapping of pea. In: Modern methods of plant analysis, vol 16. Springer, Berlin Heidelberg New York, pp 117–160

  • Ellis THN, Poyser SJ (2002) An integrated and comparative view of pea genetic and cytogenetic maps. New Phytol 153:17–25

    Article  Google Scholar 

  • Ellis THN, Turner L, Hellens RP, Lee D, Harker CL, Enard C, Domoney C, Davies DR (1992) Linkage maps in pea. Genetics 130:649–663

    CAS  PubMed  Google Scholar 

  • Frew TJ, Russell AC, Timmerman-Vaughan GM (2002) Sequence-tagged site markers linked to the sbm1 gene for resistance to Pea seed-borne mosaic virus. Plant Breed 121:512–516

    Article  CAS  Google Scholar 

  • Gal-On A, Meiri E, Elman C, Gray DJ, Gaba V (1997) Simple hand-held devices for the efficient infection of plants with viral-encoding constructs by particle bombardment. J Virol Methods 64:103–110

    Article  CAS  PubMed  Google Scholar 

  • Gritton ET, Hagedorn DJ (1975) Linkage of the genes sbm and wlo in peas. Crop Sci 15:447–448

    Google Scholar 

  • Haddad NI, Muehlbauer FJ, Hampton RO (1978) Inheritance of resistance to Pea seed-borne mosaic virus in lentils. Crop Sci 18:613–615

    Google Scholar 

  • Hagedorn DJ, Gritton ET (1971) Registration of Wisconsin 7105 and 7106 pea germplasm. Crop Sci 11:945–946

    Google Scholar 

  • Hagedorn DJ, Gritton ET (1973) Inheritance of resistance to the Pea seed-borne mosaic virus. Phytopathology 63:1130–1133

    Google Scholar 

  • Haldane JBS, Waddington CH (1931) Inbreeding and linkage. Genetics 16:357–374

    Google Scholar 

  • Hampton RO (1980) Pea seed-borne mosaic symptoms variation among Pisum plant introduction accessions: expression & variation in symptom expression among different genotypes of Pisum and the pathological implications. Pisum Newsl 12:29–30

    Google Scholar 

  • Hampton RO, Marx GA (1981) Immunity to Pea seed-borne mosaic virus: a re-assessment. Pisum Newsl 13:16–17

    Google Scholar 

  • Johansen IE, Lund OS, Hjulsager CK, Laursen J (2001) Recessive resistance in Pisum sativum and potyvirus pathotype resolved in a gene-for-cistron correspondence between host and virus. J Virol 75:6609–6614

    Article  CAS  PubMed  Google Scholar 

  • Khetarpal RK, Maury Y (1987) Pea seed-borne mosaic virus: a review. Agronomie 7:215–224

    Google Scholar 

  • Kyle MM, Palloix A (1997) Proposed revision of nomenclature for potyvirus resistance genes in Capsicum. Euphytica 97:183–188

    Article  Google Scholar 

  • Lacou V, Haurogné K, Ellis N, Rameau C (1998) Genetic mapping in pea. 1- RAPD-based genetic linkage map of Pisum sativum. Theor Appl Genet 97:905–915

    Article  Google Scholar 

  • Lellis AD, Kasschau KD, Whitham SA, Carrington JC (2002) Loss-of-susceptibility mutants of Arabidopsis thaliana reveal an essential role for eIF(iso)4E during potyvirus Infection. Curr Biol 12:1046–1051

    Article  CAS  PubMed  Google Scholar 

  • Maule AJ, Wang D (1996) Seed transmission of plant viruses: a lesson in biological complexity. Trends Microbiol 4:153–158

    Article  CAS  PubMed  Google Scholar 

  • Muehlbauer FJ (1983) Eight germplasm lines of pea resistant to Pea seed-borne mosaic virus. Crop Sci 23:1019

    Google Scholar 

  • Murray MG, Cuellar RE, Thompson WF (1978) DNA sequence organization in the pea genome. Biochemistry 17:5781-5790

    CAS  PubMed  Google Scholar 

  • Nicaise V, German-Retana S, Sanjuán R, Dubrana M-P, Mazier M, Maisonneuve B, Candresse T, Caranta C, LeGall O (2003) The eukaryotic translation initiation factor 4E controls lettuce susceptibility to the potyvirus Lettuce mosaic virus. Plant Physiol 132:1272–1282

    Article  CAS  PubMed  Google Scholar 

  • Provvidenti R, Alconero R (1988) Inheritance of resistance to a lentil strain of Pea seed-borne mosaic virus in Pisum sativum. J Hered 79:45–47

    Google Scholar 

  • Provvidenti R, Hampton RO (1991) Chromosomal distribution of gene for resistance to seven potyviruses in Pisum sativum. Pisum Genet 23:26–28

    Google Scholar 

  • Provvidenti R, Muehlbauer FJ (1990) Evidence of a cluster of linked genes for resistance to Pea seed-borne mosaic virus and Clover yellow vein virus on chromosome 6. Pisum Newsl 22:43–45

    Google Scholar 

  • Provvidenti R, Hampton RO, Muehlbauer FJ (1991) G-1000, Marx’s pea breeding line for multiviral resistance. Pisum Genet 23:50–52

    Google Scholar 

  • Ruffel S, Dussault MH, Palloix A, Moury B, Bendahmane A, Robaglia C, Caranta C (2002) A natural recessive resistance gene against Potato virus Y in pepper corresponds to the eukaryotic initiation factor 4E (eIF4E). Plant J 32:1067–1075

    Article  CAS  PubMed  Google Scholar 

  • Sambrook J, Russell DW (2001) Molecular cloning—a laboratory manual, 3rd edn. Cold Spring Harbor Laboratory Press, Cold Springer Harbor, N.Y.

  • Stam P (1993) Construction of integrated genetic linkage maps by means of a new computer package: JoinMap. Plant J 3:739–744

    CAS  Google Scholar 

  • Stam P, van Ooijen JW (1995) JoinMap, ver 2.0: software for the calculation of genetic linkage maps. CPRO-DLO, Wageningen, The Netherlands

  • Stevenson WR, Hagedorn DJ (1971) Reaction of Pisum sativum to the Pea seed-borne mosaic virus. Plant Dis Rep 55:408–409

    Google Scholar 

  • Timmerman GM, Frew TJ, Miller AL, Weeden NF, Jermyn WA (1993) Linkage mapping of sbm-1, a gene conferring resistance to Pea seed-borne mosaic virus, using molecular markers in Pisum sativum. Theor Appl Genet 85:609–615

    CAS  Google Scholar 

  • Wittmann S, Chatel H, Fortin MG, Laliberte J-F (1997) Interaction of the viral protein genome linked of Turnip mosaic potyvirus with the translational eukaryotic initiation factor(iso)4E of Arabidopsis thaliana using the yeast two-hybrid system. Virology 234:84–92

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgements

We thank Elisabeth Johansen, Roger Cousin, Boulos Chaloub, Delphine Malayah, Maggie Knox and Linda Turner for constructive discussions, and particularly Elisabeth Johansen and Roger Cousin for making available to us PSbMV cDNA clones and pea lines 735 and 844, respectively. Z.G. and S.E. were supported directly from a research grant (208/P13344) provided by the UK Biotechnology and Biological Sciences Research Council (BBSRC). The John Innes Centre is also grant-aided by the BBSRC. The work was carried out under the UK Department of Environment, Food and Rural Affairs licence numbers PHL 185/4192 and 185A/4512.

Author information

Authors and Affiliations

  1. John Innes Centre, Norwich Research Park, Colney, Norwich , NR4 7UH, UK

    Z. Gao, S. Eyers, C. Thomas, N. Ellis & A. Maule

Authors
  1. Z. Gao
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. S. Eyers
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. C. Thomas
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. N. Ellis
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. A. Maule
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to A. Maule.

Additional information

Communicated by C. Möllers

Rights and permissions

Reprints and permissions

About this article

Cite this article

Gao, Z., Eyers, S., Thomas, C. et al. Identification of markers tightly linked to sbm recessive genes for resistance to Pea seed-borne mosaic virus . Theor Appl Genet 109, 488–494 (2004). https://doi.org/10.1007/s00122-004-1652-6

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00122-004-1652-6

Keywords

Search

Navigation