Abstract
A genetic linkage map has been constructed for meadow fescue (Festuca pratensis Huds.) (2n=2x=14) using a full-sib family of a cross between a genotype from a Norwegian population (HF2) and a genotype from a Yugoslavian cultivar (B14). The two-way pseudo-testcross procedure has been used to develop separate maps for each parent, as well as a combined map. A total number of 550 loci have been mapped using homologous and heterologous RFLPs, AFLPs, isozymes and SSRs. The combined map consists of 466 markers, has a total length of 658.8 cM with an average marker density of 1.4 cM/marker. A high degree of orthology and colinearity was observed between meadow fescue and the Triticeae genome(s) for all linkage groups, and the individual linkage groups were designated 1F–7F in accordance with the orthologous Triticeae chromosomes. As expected, the meadow fescue linkage groups were highly orthologous and co-linear with Lolium, and with oat, maize and sorghum, generally in the same manner as the Triticeae chromosomes. It was shown that the evolutionary 4AL/5AL translocation, which characterises some of the Triticeae species, is not present in the meadow fescue genome. A putative insertion of a segment orthologous to Triticeae 2 at the top of 6F, similar to the rearrangement found in the wheat B and the rye R genome, was also observed. In addition, chromosome 4F is completely orthologous to rice chromosome 3 in contrast to the Triticeae where this rice chromosome is distributed over homoeologous group 4 and 5 chromosomes. The meadow fescue genome thus has a more ancestral configuration than any of the Triticeae genomes. The extended meadow fescue map reported here provides the opportunity for beneficial cross-species transfer of genetic knowledge, particularly from the complete genome sequence of rice.
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Acknowledgments
The seed of B14, accession no. 1700, was kindly provided by Dr. Valeria Negri, University of Perugia, Italy. Dr. P. Stephenson, Comparative Genetics Unit, John Innes Centre, UK, and Dr. J.C. Glaszmann, CIRAD, Montpellier, France, developed the EGRAM anchor probe sets, and provided the following probes: PSR (wheat cDNA PSR50-200, wheat gDNA PSR300-1300), RZ (rice cDNA), RGC (rice cDNA), RGG (rice gDNA), CSU (maize cDNA), SbRPG (sorghum cDNA) and PSM (pearl millet gDNA). The BCD (barley cDNA), CDO (oat cDNA), WG (wheat gDNA) and ABG (wheat gDNA) clones were provided from the Grain Genes probe repository (http://wheat.pw.usda.gov/ggpages/probes/index.html), Dr. Olin Anderson, USDA-ARS-WRRC, US. The ABLMC (Lolium multiflorum coleoptile cDNA) and the ABLGP (L. perenne gDNA) clones were provided by Dr. John Forster, La Trobe University, Australia, and Dr. Timothy Close, UC Riverside, USA, kindly provided the dehydrin clones. Dr. Fred Eickmeyer, SZ Steinach, Germany, provided the mapping data for the isozyme locus Acp-2. We gratefully acknowledge all probe contributors, and Øyvind Jørgensen, Kristin Bye Olsen and Torleiv Veum for excellent technical assistance. We thank Prof. Mike Gale for support and many useful discussions and suggestions in preparing this manuscript. This study was supported by the EU-project EGRAM (European Gramineae Mapping Project (contract No. BI04-CT97-2220), and grant no. 110733/112 from the Research Council of Norway.
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Alm, V., Fang, C., Busso, C.S. et al. A linkage map of meadow fescue (Festuca pratensis Huds.) and comparative mapping with other Poaceae species. Theor Appl Genet 108, 25–40 (2003). https://doi.org/10.1007/s00122-003-1399-5
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DOI: https://doi.org/10.1007/s00122-003-1399-5