Skip to main content
Log in

QTL analysis for ascochyta blight resistance in an intraspecific population of chickpea (Cicer arietinum L.)

  • Published:
Theoretical and Applied Genetics Aims and scope Submit manuscript

Abstract

In both controlled environment and the field, six QTLs for ascochyta blight resistance were identified in three regions of the genome of an intraspecific population of chickpea using the IDS and AUDPC disease scoring systems. One QTL-region was detected from both environments, whereas the other two regions were detected from each environment. All the QTL-regions were significantly associated with ascochyta blight resistance using either of the disease scoring systems. The QTLs were verified by multiple interval mapping, and a two-QTL genetic model with considerable epistasis was established for both environments. The major QTLs generally showed additive gene action, as well as dominance inter-locus interaction in the multiple genetic model. All the QTLs were mapped near a RGA marker. The major QTLs were located on LG III, which was mapped with five different types of RGA markers. A CLRR-RGA marker and a STMS marker flanked QTL 6 for controlled environment resistance at 0.06 and 0.04 cM, respectively. Other STMS markers flanked QTL 1 for field resistance at a 5.6 cM interval. After validation, these flanking markers may be used in marker-assisted selection to breed for elite chickpea cultivars with durable resistance to ascochyta blight. The tight linkage of RGA markers to the major QTL on LG III will allow map-based cloning of the underlying resistance genes.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1A, B.
Fig. 2.

Similar content being viewed by others

References

  • Acikgoz N, Karaca M, Er C, Meyveci K (1994) Chickpea and lentil production in Turkey. In: Muehlbauer FJ, Kaiser WJ (eds) Expanding the production and use of cool season food legumes. Kluwer Academic Publ, Dordrecht, The Netherlands, pp 388–398

  • Ahmad F, Slinkard AE, Scoles GJ (1988) Investigations into the barrier(s) to interspecific hybridization between Cicer arietinum L. and eight other annual Cicer species. Plant Breed 100:193–198

    Google Scholar 

  • Ajmonemarsan P, Monfredini G, Ludwig WF, Melchinger AE, Franceschini P, Pagnotto G, Motto M (1995) In an elite cross of maize a major quantitative trait locus controls one-fourth of the genetic variation for grain yield. Theor Appl Genet 90:415–424

    CAS  Google Scholar 

  • Basten CJ, Weir BS, Zeng ZB (2001) QTL cartographer version 1.15: a reference manual and tutorial for QTL mapping. North Carolina State University, Raleigh, North Carolina

  • Bonierbale MW, Plaisted RL, Pineda O, Tanksley SD (1994) QTL analysis of trichome-mediated insect resistance in potato. Theor Appl Genet 87:973–987

    Google Scholar 

  • Burdon RD (1977) Genetic correlation as a concept for studying genotype-environment interaction in forest tree breeding. Silvae Genet 26:168–175

    Google Scholar 

  • Campbell CL, Madden LV (1990) Temporal analysis of epidemics. I. Description and comparison of disease progress curves. In: Introduction to plant disease epidemiology. John Wiley and Sons, New York, pp 161–202

  • Chen XM, Line RF, Leung H (1998) Genome scanning for resistance-gene analogs in rice, barley and wheat by high-resolution electrophoresis. Theor Appl Genet 97:345–355

    Article  CAS  Google Scholar 

  • Cho S, Kumar J, Shultz JL, Anupama K, Tefera F, Muehlbauer FJ (2003) Mapping genes for double podding and other morphological traits in chickpea. Euphytica (in press)

  • Churchil GA, Doerge RW (1994) Empirical threshold values for quantitative trait mapping. Genetics 18:963–971

    Google Scholar 

  • Collard BCY, Ades PK, Pang ECK, Brouwer JB, Taylor PWJ (2001) Prospecting for sources of resistance to ascochyta blight in wild Cicer species. Aust Plant Pathol 30:271–276

    Google Scholar 

  • Collard BCY, Pang ECK, Ades PK, Taylor PWJ (2003) Preliminary investigation of QTLs for seedling resistance to ascochyta blight from Cicer echinospermum, a wild relative of chickpea. Theor Appl Genet 107:719–729

    Google Scholar 

  • Dey SK, Singh G (1993) Resistance to ascochyta blight in chickpea—genetic basis. Euphytica 68:147–153

    Google Scholar 

  • Eathington SR, Dudley JW, Rufener II GK (1997) Usefulness of marker-QTL associations in early generation selection. Crop Sci 37:1686–1693

    Google Scholar 

  • Falconer DS (1952) The problem of environment and selection. Am Nat 86:293–298

    Article  Google Scholar 

  • FAOSTAT Database (2002) http://apps.fao.org/

  • Feuillet C, Schachermayr G, Keller B (1997) Molecular cloning of a new receptor-like kinase gene encoded at the Lr10 disease resistance locus of wheat. Plant J 11:45–52

    CAS  PubMed  Google Scholar 

  • Flandez-Galvez H, Ford R, Pang ECK, Taylor PWJ (2003) An intraspecific linkage map of the chickpea (Cicer arietinum L.) genome based on sequence-tagged microsatellite site and resistant-gene analog markers. Theor Appl Genet 106:1447–1456

    Google Scholar 

  • Food and Agriculture Organization of the United Nations (1996) FAO production year book. FAO, Rome

  • Galvez HF, Ford R, Pang ECK, Brouwer JB, Taylor PWJ (2000) Towards durable ascochyta blight resistance in chickpea: understanding of the genetics and resistance mechanisms. In: Durable disease resistance symposium. Wageningen, The Nertherlands, pp 26

  • Galvez HF, Ford R, Pang ECK, Brouwer JB, Taylor PWJ (2003) P478: mapping and QTL analysis of ascochyta blight (Ascochyta rabiei) resistance genes in chickpea. In: Final abstracts guide for the plant, animal and microbe genomes Xth Conference, Applied Biosystems, California, USA, pp 197

  • Hammond-Kosack KE, Jones JDG (1997) Plant disease resistance genes. Annu Rev Plant Physiol Plant Mol Biol 48:575–607

    CAS  Google Scholar 

  • Haware MP, Narayana Rao J, Pundir RPS (1992) Evaluation of wild Cicer species for resistance to four chickpea diseases. Int Chickpea Newslett 27:16–18

    Google Scholar 

  • Hüttel B, Santra D, Muehlbauer FJ, Kahl G (2002) Resistance gene analogues of chickpea (Cicer arietinum L.): isolation, genetic mapping and association with a Fusarium resistance gene cluster. Theor Appl Genet 105:479–490

    Article  Google Scholar 

  • Inukai T, Zeigler RS, Sarkarung S, Bronson M, Dung LV, Kinoshita T, Nelson RJ (1996) Development of pre-isogenic lines for rice blast-resistance by marker-aided selection from a recombinant inbred population. Theor Appl Genet 93:560–567

    Article  CAS  Google Scholar 

  • Jimenez-Diaz RM, Crino P, Halila MH, Mosconi C, Trapero-Casas AT (1993) Screening for resistance to fusarium wilt and ascochyta blight in chickpea. In: Singh KB, Saxena MC (eds) Breeding for stress tolerance in cool-season food legumes. ICARDA, Aleppo, Syria, pp 77–95

  • Kanazin V, Marek LF, Shoemaker RC (1996) Resistance gene analogs are conserved and clustered in soybean. Proc Natl Acad Sci USA 93:11746–11750

    CAS  PubMed  Google Scholar 

  • Lande R, Thompson R (1990) Efficiency of marker-assisted selection in the improvement of quantitative traits. Genetics 124:743–756

    CAS  PubMed  Google Scholar 

  • Leister D, Ballvora A, Salamini F, Gebhardt C (1996) A PCR-based approach for isolating pathogen resistance genes from potato with a potential for wide application in plants. Nature Genet 14:421–429

    CAS  PubMed  Google Scholar 

  • Li ZK, Pinson SRM, Park WD, Paterson AH, Stansel JW (1997) Epistasis for three grain yield components in rice Oryza sativa L. Genetics 145:453–465

    CAS  PubMed  Google Scholar 

  • McCouch SR, Doerge RW (1995) QTL mapping in rice. Trends Genet 11:482–487

    CAS  PubMed  Google Scholar 

  • Meredith K, Bretag T, Materne M, Brouwer JB (2000) 1999 Victorian advanced chickpea trial results and implications for 2000. In: Kaminskas D, Rawlings S (eds) VIC cropping systems update 2000. Horsham-Longerenong College, Victoria, Australia, pp 77–79

  • Michelmore R (1995) Molecular approaches to manipulation of disease resistance genes. Annu Rev Phytopathol 15:393–427

    Article  Google Scholar 

  • Mohan M, Nai S, Bhagwat A, Krishna TG, Yano M, Bhatia CR, Sasaki T (1997) Genome mapping, molecular markers and marker-assisted selection in crop plants. Mol Breed 3:87–103

    CAS  Google Scholar 

  • Nene YL, Reddy MV (1987) Chickpea diseases and their control. In: Saxena MC, Singh KB (eds) The chickpea. CAB International, UK, pp 233–270

  • Paterson AH (1996) Mapping genes responsible for differences in phenotype. In: Paterson AH (ed) Genome mapping in plants. RG Landes Co, Austin Texas, pp 41–54

  • Paterson AH, Tanksley SD, Sorrells ME (1991) DNA markers in plant improvement. Adv Agron 44:39–90

    Google Scholar 

  • Porta-Puglia A, Bernier CC, Jellis GJ, Kaiser WJ, Reddy MV (1994) Screening techniques and sources of resistance to foliar diseases caused by fungi and bacteria in cool season food legumes. Euphytica 73:11–25

    Google Scholar 

  • Rajesh PN, Tekeoglu M, Gupta VS, Ranjekar PK, Muehlbauer FJ (2002) Molecular mapping and characterization of an RGA locus RGAPtokin1–2171 in chickpea. Euphytica 128:427–433

    Article  CAS  Google Scholar 

  • Reddy MV, Singh KB (1992) Registration of five chickpea germplasm lines resistant to ascochyta blight. Crop Sci 32:1079–1080

    Google Scholar 

  • Reddy MV, Singh KB (1984) Evaluation of a world collection of chickpea germplasm accessions for resistance to ascochyta blight. Plant Dis 68:900–901

    Google Scholar 

  • Ribaut JM, Hoisington D (1998) Marker-assisted selection: new tools and strategies. Trends Plant Sci 3:236–239

    Article  Google Scholar 

  • Santra DK, Tekeoglu M, Ratnaparkhe M, Kaiser WJ, Muehlbauer FJ (2000) Identification and mapping of QTLs conferring resistance to ascochyta blight in chickpea. Crop Sci 40:1606–1612

    CAS  Google Scholar 

  • SAS Institute Inc (1996) SAS/STAT user's guide. Version 6.12. SAS Institute, Cary, North Carolina

  • Scofield SR, Tobias CM, Rathjen JP, Chang JH, Lavelle, Michelmore RW, Staskawicz BJ (1996) Molecular of gene-for-gene specificity in bacterial speck disease of tomato. Science 274:2063–2065

    Article  CAS  PubMed  Google Scholar 

  • Singh KB (1997a) Chickpea (Cicer arietinum L.). Field Crops Res 53:161–170

    Article  Google Scholar 

  • Singh KB (1997b) Experience with pyramiding of ascochyta blight resistance in kabuli chickpea. In: Udupa SM, Weigand F (eds) DNA markers and breeding for resistance to ascochyta blight in chickpea. Proc Symp "Application of DNA fingerprinting for crop improvement: marker-assisted selection of chickpea for sustainable agriculture in the dry areas". ICARDA, Aleppo, Syria, pp 121–126

  • Singh KB, Reddy MV (1993) Sources of resistance to ascochyta blight in wild Cicer species. Netherlands J Plant Pathol 99:163–167

    Google Scholar 

  • Singh KB, Hawtin GC, Nene YL, Reddy MV (1981) Resistance in chickpeas to Ascochyta rabiei. Plant Dis 65:586–587

    Google Scholar 

  • Stamigna C, Mancinelli R, Crino P, Infantino A, Porta-Puglia A, Saccardo F (1998) Multiple resistance to diseases in wild relatives of chickpea (Cicer arietinum L.). In: Proc 3rd European Conf on Grain Legumes, Valladolid, Spain

  • Stuber CW, Sisco PH (1991) Marker-facilitated transfer of QTL alleles between elite inbred lines and responses in hybrids. Proc Annu Corn Sorghum Res Conf 46:104–113

    Google Scholar 

  • Tabien RE, Li Z, Paterson AH, Marchetti MA, Stansel JW, Pinson SRM (2000) Mapping of four major rice blast resistance genes from 'Lemont' and 'Teqing' and evaluation of their combinatorial effect for field resistance. Theor Appl Genet 101:1215–1225

    Article  CAS  Google Scholar 

  • Tabien RE, Li Z, Paterson AH, Marchetti MA, Stansel JW, Pinson SRM (2002) Mapping QTLs for field resistance to the rice blast pathogen and evaluating their individual and combined utility in improved varieties. Theor Appl Genet 105:313–324

    Article  Google Scholar 

  • Tang X, Frederick RD, Zhou J, Halterman DA, Jia Y, Martin GB (1996) Initiation of plant disease resistance by physical interaction of avrPto and Pto kinase. Science 274:2060–2063

    Article  CAS  PubMed  Google Scholar 

  • Tanksley SD (1993) Mapping polygenes. Annu Rev Genet 27:205–233

    CAS  PubMed  Google Scholar 

  • Tekeoglu M, Santra DK, Kaiser WJ, Muehlbauer FJ (2000) Ascochyta blight resistance in three chickpea recombinant inbred line populations. Crop Sci 40:1251–1256

    Google Scholar 

  • Tekeoglu M, Rajesh PN, Muehlbauer FJ (2002) Integration of sequence tagged microsatellite sites to the chickpea genetic map. Theor Appl Genet 105:847–854

    Article  Google Scholar 

  • Udupa SM, Baum M (2002) Genetic dissection of pathotype-specific resistance to ascochyta blight disease in chickpea (Cicer arietinum L.) using microsatellite markers. Theor Appl Genet DOI 10.1007/s00122–002–1168-x

  • Van Rheenen HA, Haware MP (1997) Studies on inheritance of ascochyta blight resistance in chickpea. In: Udupa SM, Weigand F (eds) DNA markers and breeding for resistance to ascochyta blight in chickpea. Proc Symp on "Application of DNA fingerprinting for crop improvement: marker-assisted selection of chickpea for sustainable agriculture in the dry areas". ICARDA, Aleppo, Syria, pp 115–120

  • Wang S, Basten CJ, Zeng ZB (2002) Windows QTL Cartographer Version 1.30 copyright 2001, 2002. Statistical Genetics, North Carolina State University, USA

  • Xing YZ, Tan YF, Hua JP, Sun XL, Xu CG, Zhang Q (2002) Characterization of the main effects, epistatic effects and their environmental interactions of QTLs on the genetic basis of yield traits in rice. Theor Appl Genet 105:248–257

    CAS  Google Scholar 

  • Young ND (1996) QTL mapping and quantitative disease resistance in plants. Annu Rev Phytopathol 34:479–501

    CAS  Google Scholar 

  • Yu YG, Buss GR, Maroof MAS (1996) Isolation of a superfamily of candidate disease-resistance genes in soybean based on a conserved nucleotide-binding site. Proc Natl Acad Sci USA 93:11751–11756

    CAS  PubMed  Google Scholar 

Download references

Acknowledgements

We thank Dr. JanBert Brouwer, Mr. Kevin Meredith, Dr. Chris Pittock and Jane Hill Crane (Department of Primary Industries, Victorian Institute for Dryland Agriculture, Victoria, Australia) for the provision of the mapping population and assistance in setting up the field trial. This study was funded by a Melbourne International Research Scholarship with the support of DPI, VIDA.

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to H. Flandez-Galvez.

Additional information

Communicated by P. Langridge

Rights and permissions

Reprints and permissions

About this article

Cite this article

Flandez-Galvez, H., Ades, P.K., Ford, R. et al. QTL analysis for ascochyta blight resistance in an intraspecific population of chickpea (Cicer arietinum L.). Theor Appl Genet 107, 1257–1265 (2003). https://doi.org/10.1007/s00122-003-1371-4

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00122-003-1371-4

Keywords

Navigation