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Identification and fine mapping of Pi33, the rice resistance gene corresponding to the Magnaporthe grisea avirulence gene ACE1

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Abstract.

Rice blast disease is a major constraint for rice breeding. Nevertheless, the genetic basis of resistance remains poorly understood for most rice varieties, and new resistance genes remain to be identified. We identified the resistance gene corresponding to the cloned avirulence gene ACE1 using pairs of isogenic strains of Magnaporthe grisea differing only by their ACE1 allele. This resistance gene was mapped on the short arm of rice chromosome 8 using progenies from the crosses IR64 (resistant) × Azucena (susceptible) and Azucena × Bala (resistant). The isogenic strains also permitted the detection of this resistance gene in several rice varieties, including the differential isogenic line C101LAC. Allelism tests permitted us to distinguish this gene from two other resistance genes [Pi11 and Pi-29(t)] that are present on the short arm of chromosome 8. Segregation analysis in F2 populations was in agreement with the existence of a single dominant gene, designated as Pi33. Finally, Pi33 was finely mapped between two molecular markers of the rice genetic map that are separated by a distance of 1.6 cM. Detection of Pi33 in different semi-dwarf indica varieties indicated that this gene could originate from either one or a few varieties.

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Acknowledgements.

We thank Claudia Kaye, Nourollah Ahmadi and Michel Peterschmitt for a critical review of the manuscript. YAC clones were obtained from the Japanese Ministry of Agriculture, Forestry and Fisheries (MAFF) DNA bank.

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Author notes

  1. W. Dioh

    Present address: Monsanto, Saint Louis, Missouri, USA,

Authors and Affiliations

  1. UMR BGPI, CIRAD, TA73-09, 34398 Montpellier CEDEX, France

    R. Berruyer, H. Adreit, J. Milazzo, S. Gaillard & D. Tharreau

  2. UMR GACA, CIRAD, TA80-03, 34398 Montpellier CEDEX, France

    A. Berger

  3. IGM, CNRS-URA 2255, Orsay, France

    W. Dioh

  4. UMR1932, CNRS-Bayer Crop Science, 69269 Lyon, France

    M.-H. Lebrun

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  1. R. Berruyer
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  2. H. Adreit
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  3. J. Milazzo
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  5. A. Berger
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  8. D. Tharreau
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Correspondence to D. Tharreau.

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Communicated by D.J. Mackill

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Berruyer, R., Adreit, H., Milazzo, J. et al. Identification and fine mapping of Pi33, the rice resistance gene corresponding to the Magnaporthe grisea avirulence gene ACE1 . Theor Appl Genet 107, 1139–1147 (2003). https://doi.org/10.1007/s00122-003-1349-2

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  • DOI: https://doi.org/10.1007/s00122-003-1349-2

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