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Construction of an intraspecific linkage map of lentil (Lens culinaris ssp. culinaris)

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Abstract.

The first intraspecific linkage map of the lentil genome was constructed with 114 molecular markers (100 RAPD, 11 ISSR and three RGA) using an F2 population developed from a cross between lentil cultivars ILL5588 and ILL7537 which differed in resistance for ascochyta blight. Linkage analysis at a LOD score of 4.0 and a maximum recombination fraction of 0.25 revealed nine linkage groups comprising between 6 and 18 markers each. The intraspecific map spanned a total length of 784.1 cM. The markers were distributed throughout the genome, however markers were clustered in the middle or near the middle of the linkage groups, suggesting the location of centromeres. Of 114 mapped markers, 16 (14.0%) were distorted, usually at the end or middle of the linkage groups. The utility of ISSR and RGA markers for mapping in lentil was explored, and the primer with an (AC) repeat motif was found to be useful.

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References

  • Arumuganathan K, Earle ED (1991) Nuclear DNA content of some important plant species. Plant Mol Biol Rep 9:208–218

    CAS  Google Scholar 

  • Burr B, Burr FA, Thompson KH, Albertson MC, Stuber CW (1988) Gene mapping with recombinant inbreds in maize. Genetics 118:519–526

    CAS  PubMed  Google Scholar 

  • Causse MA, Fulton TM, Cho YG, Ahn SN, Chunwongse J, Wu K, Xiao J, Yu Z, Ronald PC, Harrington SE, Second G, McCouch SR, Tanksley SD (1994) Saturated molecular map of the rice genome based on an interspecific backcross population. Genetics 138:1251–1274

    CAS  PubMed  Google Scholar 

  • Chen XM, Line RF, Leung H (1998) Genome scanning for resistance-gene analogs in rice, barley, and wheat by high-resolution electrophoresis. Theor Appl Genet 97:345–355

    Article  CAS  Google Scholar 

  • Emami MK, Sharma B (1999) Linkage between three morphological markers in lentil. Plant Breed 118:579–581

    Article  Google Scholar 

  • Eujayl I, Baum M, Erskine W, Pehu E, Muehlbauer FJ (1997) The use of RAPD markers for lentil genetic mapping and the evaluation of distorted F2 segregation. Euphytica 96:405–412

    Article  CAS  Google Scholar 

  • Eujayl I, Baum M, Powell W, Erskine W, Pehu E (1998) A genetic linkage map of lentil (Lens sp.) based on RAPD and AFLP markers using recombinant inbred lines. Theor Appl Genet 97:83–89

    Article  CAS  Google Scholar 

  • Flandez-Galvez H, Ford R, Pang ECK, Taylor PWJ (2003) An intraspecific linkage map of the chickpea (Cicer arietinum L.) genome based on sequence tagged microsatellite site and resistance gene analog markers. Theor Appl Genet 106:1447–1456

    Google Scholar 

  • Ford R, Pang ECK, Taylor PWJ (1999) Genetics of resistance to ascochyta blight (Ascochyta lentis) of lentil and the identification of closely linked RAPD markers. Theor Appl Genet 98:93–98

    CAS  Google Scholar 

  • Freyre R, Skroch PW, Geffroy V, Adam-Blondon AF, Shirmohamadali A, Johnson WC, Llaca V, Nodari RO, Pereira PA, Tsai SM, Tohme J, Dron M, Nienhuis J, Vallejos CE, Gepts P (1998) Towards an integrated linkage map of common bean. 4. Development of a core linkage map and alignment of RFLP maps. Theor Appl Genet 97:847–856

    CAS  Google Scholar 

  • Graham MA, Marek LF, Shoemaker RC (2002) PCR sampling of disease resistance-like sequences from a disease resistance gene cluster in soybean. Theor Appl Genet 105:50–57

    Article  Google Scholar 

  • Hall KJ, Parker JS, Ellis THN (1997a) The relationship between genetic and cytogenetic maps of pea. I. Standard and translocation karyotypes. Genome 40:744–754

    Google Scholar 

  • Hall KJ, Parker JS, Ellis THN, Turner L, Knox MR, Hofer JMI, Lu J, Ferrandiz C, Hunter PJ, Taylor JD, Baird K (1997b) The relationship between genetic and cytogenetic maps of pea. II. Physical maps of linkage mapping populations. Genome 40:755–769

    CAS  Google Scholar 

  • Havey MJ, Muehlbauer FJ (1989) Linkages between restriction fragment length, isozyme, and morphological markers in lentil. Theor Appl Genet 77:395–401

    CAS  Google Scholar 

  • Hoque ME, Mishra SK, Sarker A (2002) Inheritance and linkage relationship between morphological and RAPD markers in lentil (Lens culinaris Medik.). Indian J Genet 62:5–10

    CAS  Google Scholar 

  • Huettel B, Santra D, Muehlbauer FJ, Kahl G (2002) Resistance gene analogues of chickpea (Cicer arietinum L.): isolation, genetic mapping and association with a Fusarium resistance gene cluster. Theor Appl Genet 105:479–490

    Article  Google Scholar 

  • Hüttel B, Winter P, Weising K, Choumane W, Weigand F, Kahl G (1999) Sequence-tagged microsatellite site markers for chickpea (Cicer arietinum L). Genome 42:210–217

    PubMed  Google Scholar 

  • Jenczewski E, Gherardi M, Bonnin I, Prosperi JM, Olivieri I, Huguet T (1997) Insight on segregation distortions in two intraspecific crosses between annual species of Medicago (Leguminosae). Theor Appl Genet 94:682–691

    Google Scholar 

  • Kaga A, Ohnishi M, Ishii T, Kamijima O (1996) A genetic linkage map of azuki bean constructed with molecular and morphological markers using an interspecific population (Vigna angularis × V. nakashimae). Theor Appl Genet 93:658–663

    CAS  Google Scholar 

  • Kaló P, Endre G, Zimányi L, Csanádi G, Kiss GB (2000) Construction of an improved linkage map of diploid alfalfa (Medicago sativa). Theor Appl Genet 100:641–657

    Article  Google Scholar 

  • Kanazin V, Marek LF, Shoemaker RC (1996) Resistance gene analogs are conserved and clustered in soybean. Proc Natl Acad Sci USA 93:11,746–11,750

    Article  Google Scholar 

  • Keim P, Diers BW, Olson TC, Shoemaker RC (1990) RFLP mapping in soybean: association between marker loci and variation in quantitative traits. Genetics 126:735–742

    CAS  PubMed  Google Scholar 

  • Kojima T, Nagaoka T, Noda K, Ogihara Y (1998) Genetic linkage map of ISSR and RAPD markers in Einkorn wheat in relation to that of RFLP markers. Theor Appl Genet 96:37–45

    Article  CAS  Google Scholar 

  • Kosambi DD (1944) The estimation of map distances from recombination values. Ann Eugen 12:172–175

    Google Scholar 

  • Lambrides CJ, Lawn RJ, Godwin ID, Manners J, Imrie BC (2000) Two genetic linkage maps of mungbean using RFLP and RAPD markers. Aust J Agric Res 51:415–425

    CAS  Google Scholar 

  • Lander ES, Green P, Abrahamson J, Barlow A, Daly J, Lincoln SE, Newsburg L (1987) mapmaker: an interactive computer package for constructing primary genetic linkage maps of experimental populations. Genomics 1:174–181

    CAS  PubMed  Google Scholar 

  • Laucou V, Haurogné K, Ellis N, Rameau C (1998) Genetic mapping in pea. 1. RAPD-based genetic linkage map of Pisum sativum. Theor Appl Genet 97:905–915

    Article  Google Scholar 

  • Lefebvre V, Palloix A, Caranta C, Pochard E (1995) Construction of an intraspecific integrated linkage map of pepper using molecular markers and doubled-haploid progenies. Genome 38:112–121

    CAS  Google Scholar 

  • Masojć P, Myśków B, Milczarski P (2001) Extending a RFLP-based genetic map of rye using random amplified polymorphic DNA (RAPD) and isozyme markers. Theor Appl Genet 102:1273–1279

    Article  Google Scholar 

  • Menéndez CM, Hall AE, Gepts P (1997) A genetic linkage map of cowpea (Vigna unguiculata) developed from a cross between two inbred, domesticated lines. Theor Appl Genet 95:1210–1217

    Article  Google Scholar 

  • Muehlbauer FJ, Weeden NF, Hoffman DL (1989) Inheritance and linkage relationships of morphological and isozyme loci in lentil (Lens Miller). J Hered 80:298–303

    Google Scholar 

  • Nagaoka T, Ogihara Y (1997) Applicability of inter-simple sequence repeat polymorphisms in wheat for use as DNA markers in comparison to RFLP and RAPD markers. Theor Appl Genet 94:597–602

    CAS  Google Scholar 

  • Nguyen TT, Taylor PWJ, Brouwer JB, Pang ECK, Ford R (2001) A novel source of resistance in lentil (Lens culinaris ssp. culinaris) to ascochyta blight caused by Ascochyta lentis. Aust Plant Pathol 30:211–215

    Article  Google Scholar 

  • Nodari RO, Tsai SM, Gilbertson RL, Gepts P (1993) Towards an integrated linkage map of common bean. 2. Development of an RFLP-based linkage map. Theor Appl Genet 85:513–520

    CAS  Google Scholar 

  • O'Brien SJ (1993) Genetic maps: locus maps of complex genomes, 6th edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor

    Google Scholar 

  • Paran I, Goldman I, Tanksley SD, Zamir D (1995) Recombinant inbred lines for genetic mapping in tomato. Theor Appl Genet 90:542–548

    CAS  Google Scholar 

  • Paterson AH, Tanksley SD, Sorrells ME (1991) DNA markers in plant improvement. Adv Agron 46:39–90

    CAS  Google Scholar 

  • Ratnaparkhe MB, Santra DK, Tullu A, Muehlbauer FJ (1998) Inheritance of inter-simple-sequence-repeat polymorphisms and linkage with a fusarium wilt resistance gene in chickpea. Theor Appl Genet 96:348–353

    Article  CAS  Google Scholar 

  • Saliba-Colombani V, Causse M, Gervais L, Philouze J (2000) Efficiency of RFLP, RAPD, and AFLP markers for the construction of an intraspecific map of the tomato genome. Genome 43:29–40

    Article  CAS  PubMed  Google Scholar 

  • Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning. A laboratory manual, 2nd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor

  • Santra DK, Tekeoglu M, Ratnaparkhe M, Kaiser WJ, Muehlbauer FJ (2000) Identification and mapping of QTLs conferring resistance to ascochyta blight in chickpea. Crop Sci 40:1606–1612

    CAS  Google Scholar 

  • Simon CJ, Muehlbauer FJ (1997) Construction of a chickpea linkage map and its comparison with maps of pea and lentil. J Hered 88:115–119

    CAS  Google Scholar 

  • Tadmor Y, Zamir D, Ladizinsky G (1987) Genetic mapping of an ancient translocation in the genus Lens. Theor Appl Genet 73:883–892

    Google Scholar 

  • Tahir M, Muehlbauer FJ (1994) Gene mapping in lentil with recombinant inbred lines. J Hered 85:306–310

    CAS  Google Scholar 

  • Tahir M, Simon CJ, Muehlbauer FJ (1993) Gene map of lentil: a review. Lens Newsletter 20:3–10

    Google Scholar 

  • Tanksley SD, Ganal MW, Prince JP, de Vicente MC, Bonierbale MW, Broun P, Fulton TM, Giovannoni JJ, Grandillo S, Martin GB, Messeguer R, Miller JC, Miller L, Paterson AH, Pineda O, Röder MS, Wing RA, Wu W, Young ND (1992) High density molecular linkage maps of the tomato and potato genomes. Genetics 132:1141–1160

    CAS  PubMed  Google Scholar 

  • Taylor BA (1978) Recombinant inbred strains: use in gene mapping. In: Morse HC (ed) Origins of inbred mice. Academic Press, New York, pp 423–438

  • Taylor PWJ, Fraser TA, Ko HL, Henry RJ (1995) RAPD analysis of sugarcane during tissue culture. In: Terzi M, Cella R, Falavigna A (eds) Current issues in plant molecular and cellular biology. Kluwer, Dordrecht, pp 241–246

  • Thoquet P, Ghérardi M, Journet EP, Kereszt A, Ané JM, Prosperi JM, Huguet T (2002) The molecular genetic linkage map of the model legume Medicago truncatula: an essential tool for comparative legume genomics and the isolation of agronomically important genes. BMC Plant Biol 2:1

    Google Scholar 

  • Timmerman-Vaughan GM, Frew TJ, Weeden NF (2000) Characterization and linkage mapping of R-gene analogous DNA sequences in pea (Pisum sativum L.). Theor Appl Genet 101:241–247

    CAS  Google Scholar 

  • Vaillancourt RE, Slinkard AE (1993) Linkage of morphological and isozyme loci in lentil, Lens culinaris L. Can J Plant Sci 73:917–926

    CAS  Google Scholar 

  • Wang Z, Weber JL, Zhong G, Tanksley SD (1994) Survey of plant short tandem DNA repeats. Theor Appl Genet 88:1–6

    CAS  Google Scholar 

  • Weeden NF, Muehlbauer FJ, Ladizinsky G (1992) Extensive conservation of linkage relationships between pea and lentil genetic maps. J Hered 83:123–129

    Google Scholar 

  • Welsh J, McClelland M (1990) Fingerprinting genomes using PCR with arbitrary primers. Nucleic Acids Res 18:7213–7218

    PubMed  Google Scholar 

  • Williams JGK, Kubelik AR, Livak KJ, Rafalski JA, Tingey SV (1990) DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res 18:6531–6535

    PubMed  Google Scholar 

  • Winter P, Benko-Iseppon AM, Hüttel B, Ratnaparkhe M, Tullu A, Sonnante G, Pfaff T, Tekeoglu M, Santra D, Sant VJ, Rajesh PN, Kahl G, Muehlbauer FJ (2000) A linkage map of the chickpea (Cicer arietinum L.) genome based on recombinant inbred lines from a C. arietinum × C. reticulatum cross: localization of resistance genes for fusarium wilt races 4 and 5. Theor Appl Genet 101:1155–1163

    CAS  Google Scholar 

  • Zamir D, Ladizinsky G (1984) Genetics of allozyme variants and linkage groups in lentil. Euphytica 33:329–336

    CAS  Google Scholar 

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Acknowledgements.

The project is funded by a Melbourne International Research Scholarship from The University of Melbourne, with the support of the Department of Primary Industries, Victorian Institute for Dryland Agriculture, Horsham, Australia.

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  1. BioMarka, Joint Centre for Crop Innovation, School of Agriculture and Food Systems, The University of Melbourne, Parkville, Victoria, Australia 3010

    Rubeena, R. Ford & P. W. J. Taylor

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  1. Rubeena
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  2. R. Ford
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  3. P. W. J. Taylor
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Correspondence to Rubeena.

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Communicated by H.C. Becker

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Rubeena, ., Ford, R. & Taylor, P.W.J. Construction of an intraspecific linkage map of lentil (Lens culinaris ssp. culinaris). Theor Appl Genet 107, 910–916 (2003). https://doi.org/10.1007/s00122-003-1326-9

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  • DOI: https://doi.org/10.1007/s00122-003-1326-9

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