Abstract.
The uses of hexaploid tritordeum as a crop for human consumption require improvement of its bread-making quality. For this purpose chromosome 1D of bread wheat with the Glu-D1 allele encoding for high-molecular-weight glutenin subunits Dx5+Dy10 was introgressed into tritordeum. Different primary tritordeums were crossed with wheats carrying subunits Dx5+Dy10. The hybrids were backcrossed to tritordeum and seeds for the next backcross (or selfing) were selected for the presence of chromosome 1D using SDS-PAGE. Forty two chromosome plants carrying subunits Dx5+Dy10 were obtained after two backcrosses and selfing. Chromosome characterization of these plants using fluorescence in situ hybridisation (FISH) proved that either chromosome substitution 1Hch/1D or 1A/1D had been obtained. A homozygous plant with a translocation of the entire 1DL arm to 1HchS was also obtained. The complete chromosome substitution lines have better agronomic characteristics than the lines with translocations.
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Ballesteros, J., Alvarez, J.B., Giménez, M.J. et al. Introgression of 1Dx5+1Dy10 into Tritordeum. Theor Appl Genet 106, 644–648 (2003). https://doi.org/10.1007/s00122-002-1110-2
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DOI: https://doi.org/10.1007/s00122-002-1110-2