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A two-dimensional electrophoresis reference map of human ovary

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Abstract

The ovary plays a central role in oogenesis and gonadal hormone secretion. Proteomic analysis is a valuable approach for gaining an increased understanding of the molecular nature of the ovary. In this work, two-dimensional electrophoresis for protein separation followed by matrix-assisted laser desorption/ionization mass spectrometry and database searches, identified 231 protein spots corresponding to 138 individual proteins that were found in gels representing both the follicular and luteal phases. The data were used to construct a database online (http://reprod.njmu.edu.cn/2d). The identified proteins were functionally classified into seven groups: (1) cell signaling/communication, (2) cell division, (3) gene/protein expression, (4) metabolism, (5) cell structure and motility, (6) cell/organism defense, and (7) unclassified. Among the proteins identified, 47% had not been previously reported in the human ovary. In addition, a number of disease-related proteins were identified in this protein map, including some cancer- and polycystic ovarian syndrome-related proteins. Two proteins with phosphorylation were verified by Western blot analysis. Comparison of protein abundance between follicular and luteal stages produced seven protein spots that had been identified in our database. This study provides a preliminary reference map of normal human ovary that will form a basis for comparative studies on normal and pathological conditions of the human ovary and may serve as a potential tool for clinical diagnosis, therapeutics, and prognosis.

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Abbreviations

PCOS:

polycystic ovarian syndrome

2-D PAGE:

two-dimensional polyacrylamide gel electrophoresis

CHAPS:

3-[(3-cholamidopropyl)-dimethylammonio]-1-propane sulfonate

IPG:

immobilized pH gradient

SDS:

sodium dodecyl sulphate

APS:

ammonium persulfate

TEMED:

N,N,N′,N′-tetramethylethylenediamine

ACN:

acetonitrile

TFA:

trifluoroacetic acid

DTT:

dithiothreitol

hnRNP A2/B1:

heterogeneous nuclear ribonucleoproteins A2/B1

IEF:

Isoelectric focusing

MALDI-TOF:

matrix-assisted laser desorption/ionization time-of-flight

pI:

isoelectric points

MWs:

molecular weights

ANX:

annexin

HCC:

hepatocellular carcinoma

VDAC:

voltage-dependent anion-selective channel

BAK:

BCL2 homologous antagonist/killer

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (30170356).

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Authors and Affiliations

  1. Laboratory of Reproductive Medicine, Nanjing Medical University, 140 Hanzhong Road, Nanjing, Jiangsu Province, 210029, People’s Republic of China

    Lei Wang, Ye-Fei Zhu, Xue-Jiang Guo, Ran Huo, Xiang Ma, Min Lin, Zuo-Min Zhou & Jia-Hao Sha

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  1. Lei Wang
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  2. Ye-Fei Zhu
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  3. Xue-Jiang Guo
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  4. Ran Huo
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  6. Min Lin
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Correspondence to Jia-Hao Sha.

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L. Wang and Y.-F. Zhu contributed equally to this work

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Wang, L., Zhu, YF., Guo, XJ. et al. A two-dimensional electrophoresis reference map of human ovary. J Mol Med 83, 812–821 (2005). https://doi.org/10.1007/s00109-005-0676-y

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