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The efficacy of platelet-rich plasma gel in MRSA-related surgical wound infection treatment: an experimental study in an animal model

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European Journal of Trauma and Emergency Surgery Aims and scope Submit manuscript

Abstract

Introduction

The wound healing properties of platelet-rich plasma (PRP) gel have been documented in many studies. PRP gel has also become a promising agent for treating surgical site infections. In this study, we investigated the antibacterial activity and wound healing effectiveness of PRP in an animal model of Methicillin-resistant Staphylococcus aureus subsp. aureus (MRSA N315)-contaminated superficial soft tissue wounds.

Materials and methods

Subcutaneous wounds in Wistar Albino male rats were created by making two cm midline incisions followed by inoculation of microorganisms. Study groups comprised of Sham (no treatment), PRP alone, MRSA alone, MRSA + PRP, MRSA + Vancomycin, and MRSA + Vancomycin + PRP groups. We inoculated 0.1 mL (3 × 108 CFU/mL) of MRSA in contaminated groups. After 8 days, all rats were killed, wounds were excised and subjected to histopathologic examination, and MRSA counts were determined.

Results

MRSA counts in MRSA, MRSA + PRP, MRSA + Vancomycin and MRSA + Vancomycin + PRP groups were 5.1 × 106 (SD ± 0.4) CFU/mL, 4.3 × 106 (SD ± 0.7) CFU/mL, 2.3 × 106 (SD ± 0.3) CFU/mL, 1.1 × 106 (SD ± 0.4) CFU/mL, respectively. The inflammation scores of MRSA + PRP, MRSA + Vancomycin, and MRSA + Vancomycin + PRP groups were significantly lower than the MRSA group. MRSA + Vancomycin + PRP group inflammation score was significantly lower than the MRSA + PRP group.

Discussion

All treatment groups were effective in wound healing and decreasing the MRSA counts. MRSA + PRP combined created identical inflammation scores to the PRP group. More in vivo studies are required to corroborate these findings.

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Author information

Authors and Affiliations

  1. Department of Infectious Disease, Sultan Abdulhamid Han Training and Education Hospital, University of Health Science, Istanbul, Turkey

    R. A. Cetinkaya

  2. Blood and Training Center, Gulhane Training and Education Hospital, University of Health Science, Ankara, Turkey

    S. Yilmaz

  3. Department of War Surgery, Gulhane Training and Education Hospital, University of Health Science, Ankara, Turkey

    A. Ünlü

  4. Department of Surgery, NYU Winthrop Hospital, 222 Station Plaza North, Suite 603, Mineola, NY, 11501, USA

    P. Petrone

  5. New York Medical College, Valhalla, USA

    P. Petrone & C. Marini

  6. Universidad de Las Palmas de Gran Canaria, Las Palmas, Spain

    P. Petrone

  7. Turkish Pharmaceutical and Medical Device Institution, Ankara, Turkey

    E. Karabulut

  8. Department of General Surgery, Gulhane Training and Education Hospital, University of Health Science, Ankara, Turkey

    M. Urkan

  9. Department of Cardiovascular Surgery, Gaziantep University, Gaziantep, Turkey

    E. Kaya

  10. Department of Cardiovascular Surgery, Gulhane Training and Education Hospital, University of Health Science, Ankara, Turkey

    K. Karabacak

  11. Biochemistry Laboratory, Corlu State Hospital, Tekirdag, Turkey

    M. Uyanik

  12. Department of Pediatrics, Afyon Kocatepe University, Afyon, Turkey

    I. Eker

  13. Yaman İs OSGB, Cankaya, Ankara, Turkey

    A. Kilic

  14. Department of Pathology, Kırıkkale Yuksek Ihtisas Hospital, Kırıkkale, Turkey

    A. Gunal

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  1. R. A. Cetinkaya
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  2. S. Yilmaz
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  3. A. Ünlü
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  4. P. Petrone
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  6. E. Karabulut
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  7. M. Urkan
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Corresponding author

Correspondence to P. Petrone.

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Conflict of interest

Riza Aytac Cetinkaya, Soner Yilmaz, Aytekin Ünlü, Patrizio Petrone, Corrado P. Marini, Enes Karabulut, Murat Urkan, Erkan Kaya, Kubilay Karabacak, Metin Uyanik, Ibrahim Eker, Abdullah Kilic, Armağan Gunal declare that they have not conflict of interest.

Informed consent

This research involved animals as subjects of the study. The study design for animal procedures was approved by the Ethics Committee of Gulhane Military Medical Academy.

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Cetinkaya, R.A., Yilmaz, S., Ünlü, A. et al. The efficacy of platelet-rich plasma gel in MRSA-related surgical wound infection treatment: an experimental study in an animal model. Eur J Trauma Emerg Surg 44, 859–867 (2018). https://doi.org/10.1007/s00068-017-0852-0

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  • DOI: https://doi.org/10.1007/s00068-017-0852-0

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