Abstract.
Brefeldin A, an inhibitor of protein secretion, caused typical alterations to the endomembrane system with limited effects on viability when given to unorganized carrot cells growing in suspension. When given to the same cells during particular stages of embryogenesis, it caused similar endomembrane lesions and an almost complete arrest of the embryogenic process. Addition of conditioned medium containing extracellular secreted proteins to the embryos during treatment with Brefeldin A allowed acquisition of polarity and the continuation of a quasi-normal embryogenic process.
Similar content being viewed by others
Author information
Authors and Affiliations
Additional information
Received: 4 December 1996 / Accepted: 4 March 1997
Rights and permissions
About this article
Cite this article
Capitanio, G., Baldan, B., Filippini, F. et al. Morphogenetic effects of Brefeldin A on embryogenic cell cultures of Daucus carota L.. Planta 203, 121–128 (1997). https://doi.org/10.1007/s00050172
Issue Date:
DOI: https://doi.org/10.1007/s00050172