Abstract
A microbial isolate showed strong antibacterial and antifungal activity against various multidrug-resistant test organisms. Based on physiological and biochemical characteristics and 16S ribosomal RNA sequence homology studies, it was found to be similar to Streptomyces capoamus (gene sequence similarity 98%). The antifungal metabolite was produced majorly intracellularly. The active metabolite was extracted and purified by gel filtration chromatography and high-performance liquid chromatography (HPLC). Partial chemical characterization of the active compound has been completed. To the best of our knowledge this strain has not been reported to produce antifungal compounds. The paper presented here describes the activity profile of the strain, classical medium optimization, and purification of the antifungal compound.
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References
Banna NE, Winkelmann G (1998) Pyrrolnitrin from Burkholderia cepacia: antibiotic activity against fungi and novel activities against streptomycetes. J Appl Microbiol 85:69–78
Bieber LW, Da Silva Filho AA, De-Mello JF, De Lima OG, Do Nascimento MS, Veith HJ, Saal VD (1987) Desaminoanthracyclines from the antibiotic complex ciclamycin. J Antibiot 40:1335–1338
Boeck LD, Clem GM, Wilson MM, Westhead JE (1973) A9145, a new adenine-containing antifungal antibiotic: fermentation. Antimicrob Agent Chemother 3:49–56
Castillo UF, Strobel GA, Ford EJ, Hess WM, Porter H, Jensen JB, Albert H, Robinson R, Condron MAM, Teplow DB, Stevens D, Yaver D (2002) Munumbicins, wide-spectrum antibiotics produced by Streptomyces NRRL 30562, endophytic on Kennedia nigriscans. Microbiology 148:2675–2685
Cragg GM, Newman DJ, Snader KM (1997) Natural products in drug discovery and development. J Nat Prod 60:52–60
Fukuda T, Matsumoto A, Takahashi Y, Tomoda H, Omura S (2005) Phenatic acids A and B, new Potentiators of antifungal miconazole activity produced by Streptomyces sp. K03–0132. J Antibitot 58:252–259
Fushimi S, Nishikawa S, Shimazu A, Seto H (1989) Studies on new phosphate ester antifungal antibiotics phoslactomycins. J Antibitot 42:1019–1025
Gonclaves DL, Delle MF, D’Albuquerque IL, Marini Bettolo GB (1968) Identification of ciclacidine antibiotic from S. capoamus. Tetrahedron Lett 4:471–473
Hayakawa Y, Iwakiri T, Imamura K, Seto H, Otake N (1985) Studies on the isotetracenone antibiotics I. Capoamycin, a new antitumor antibiotic. J Antibiot 38:957–959
Hedge VR, Puar MS, Dai P, Pu H, Patel M, Anthes JC, Richard C, Terracciano J, Das PR, Gullo V (2001) A family of depsi-peptide fungal metabolite, as selective and competitive human tachykinin receptor (NK2) antagonists: fermentation, isolation and physico-chemical properties, and biological activity. J Antibiot 54:125–135
Martins CS, Maior AMS (2003) Anthracycline production by Streptomyces capoamus in batch fermentation. Braz Arch Boil Technol 46:483–488
Marwick JD, Wright PC, Burgess JG (1999) Bioprocess intensification for production of novel marine bacterial antibiotics through bioreactor operation and design. Mar Biotechnol 1:495–507
Moncheva P, Tishkov S, Dimitrova N, Chipeva V, Nikolova SA, Bogatzevska N (2000–2002) Characteristics of soil actinomycetes from Antarctica. J Culture Collection 3:3–14
Paradkar VR, Gupte TE, Joshi AP, Naik SR (1998) A novel Streptoverticillium cinnamoneum var scleroticum producing a polyene antibiotic. World J Microbiol Biotechnol 14:705–709
Qureshi A, Mauger JB, Cano RJ, Galazzo JL, Lee MD (2001) MF-EA-705α & MF-EA-705β, new metabolites from microbial fermentation of a Streptomyces sp. J Antibiot 54:1100–1103
Sharma YR (2001) Elementary organic spectroscopy: Principles and chemical applications. S Chand, New Delhi
Silverstein RM, Webster FX (1998) Spectrometric Identification of Organic Compounds. Ed.6th, John Wiley, Canada, pp 2–144
Strobel RJ, Sullivan GR (1999) Experimental design for improvement of fermentation. In: Demain AL Davies JE (eds) Manual of industrial microbiology and biotechnology, ASM Press, Washington, pp 80–93
Ueki M, Abe K, Hanafi M, Shibata K, Tanaka T, Taniguchi M (1996) UK-2A, B, C, D. Novel antifungal antibiotics from Streptomyces sp. 517–02 I. Fermentation Isolation and Biogical Properties. J Antibiot 49:639–643
Upadhyay R, Visintin L, Jayaswal R (1991) Environmental factor affecting antagonisms of Pseudomonas cepacia against Trichoderma viride. Can J Microbiol 37:880–884
Vahidi H, Kobarfard F, Namjoyan F (2004) Effect of cultivation conditions on growth and antifungal activity of Mycena leptocephala. Af J Biotechnol 3:606–609
Ziuzina ML, Efimova TP (1979) Effect of inorganic phosphate on levorin biosynthesis and on the mycelial makeup of Streptomyces levoris. Antibiotiki 24:656–659
Acknowledgements
The authors would like to thank Mr. D. N. Vishwakarma (STO), Medicinal and Process Chemistry Division, CDRI for HPLC purification and the Council of Scientific and Industrial Research (CSIR), India for financial support.
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Singh, V., Tripathi, C.K.M. & Bihari, V. Production, optimization and purification of an antifungal compound from Streptomyces capoamus MTCC 8123. Med Chem Res 17, 94–102 (2008). https://doi.org/10.1007/s00044-007-9040-9
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DOI: https://doi.org/10.1007/s00044-007-9040-9