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Different functional outcomes of intercellular membrane transfers to monocytes and T cells

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Abstract

Trogocytosis is the uptake of membranes from one cell by another. Trogocytosis has been demonstrated for monocytes, B cells, T cells, and NK cells. The acquisition of the tolerogenic molecule HLA-G by T cells and NK cells makes them behave as regulatory cells. We investigated here whether HLA-G, which is expressed by tumor cells in vivo, could be acquired by monocytes and if this transfer could have functional consequences. We demonstrate that resting, and even more so, activated monocytes efficiently acquire membrane-bound HLA-G from HLA-G tumor cells by trogocytosis. However, we demonstrate that HLA-G quickly disappears from the surface of the monocytes in contrast to the HLA-G acquired by T cells. Consequently, HLA-Gacq+ monocytes do not reliably inhibit the on-going proliferation of autologous activated T cells and do not inhibit their cytokine production. Thus, we show that the acquirer cell may control the functional outcome of trogocytosis.

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Acknowledgments

This work was supported by the Commisariat a l’Energie Atomique (CEA) and Fondo de Investigacion Sanitaria PI070298.

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Authors and Affiliations

  1. CEA, I2BM, Service de Recherches en Hemato-Immunologie, 75475, Paris, France

    Kiave-Yune HoWangYin, Estibaliz Alegre, Marina Daouya, Benoit Favier, Edgardo D. Carosella & Joel LeMaoult

  2. Institut Universitaire d’Hematologie, Hopital Saint Louis, 75475, Paris, France

    Kiave-Yune HoWangYin, Marina Daouya, Benoit Favier, Edgardo D. Carosella & Joel LeMaoult

  3. Department of Biochemistry, University Clinic of Navarra, Pamplona, Spain

    Estibaliz Alegre

  4. CEA-I2BM-SRHI, Hopital Saint Louis, 1 Avenue Claude Vellefaux, 75010, Paris, France

    Joel LeMaoult

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  1. Kiave-Yune HoWangYin
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  2. Estibaliz Alegre
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  3. Marina Daouya
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  4. Benoit Favier
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  5. Edgardo D. Carosella
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  6. Joel LeMaoult
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Correspondence to Joel LeMaoult.

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K.-Y. HoWangYin and E. Alegre contributed equally to this work.

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Supplemental Figure 1: Intracellular expression of HLA-G

Intra-cytoplasmic expression of HLA-G by monocytes was assessed by intracellular staining using the antibody anti-HLA-G 4H84. Data are representative of 3 independent experiments. (TIFF 2194 kb)

Supplemental Figure 2: Trogocytosis is not inhibited by blocking HLA-G or ILT2

Acquisition of HLA-G by monocytes incubated 30 min with M8-HLA-G1 in the presence of a blocking anti-HLA-G1 (87G), or blocking anti-ILT2 (GHI/75), or isotypic control antibodies. Data are representative of three independent experiments. (TIFF 1904 kb)

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HoWangYin, KY., Alegre, E., Daouya, M. et al. Different functional outcomes of intercellular membrane transfers to monocytes and T cells. Cell. Mol. Life Sci. 67, 1133–1145 (2010). https://doi.org/10.1007/s00018-009-0239-4

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  • DOI: https://doi.org/10.1007/s00018-009-0239-4

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