Abstract:
Objective and design: To examine the hypothesis that an accelerated rate of neutrophil apoptosis occurs following β 2-integrin activation, and further investigate the signal transduction pathways involved.¶Material: Human polymorphonuclear neutrophils.¶Treatment: Neutrophils were challenged with pansorbins coated with antibodies towards the β 2-integrin subunit CD18 in a proportion of 1:100 with or without the inhibitors diphenylene iodonium (10 M), cytochalasin B (5 μg/ml), genistein (10 nM), herbimycin A (10 M) and Z-VAD-FMK (10 μM).¶Methods: Measurement of phosphatidylserine exposure and DNA fragmentation in flow cytometry and assessment of H2O2-production through spectrofluorometry. The results were analysed using Mann Whitney U test and Kruskal Wallis.¶Results: Pansorbins coated with antibodies to CD18 induce apoptosis in neutrophils (p<0.01), and activate the production of reactive oxygen species (p<0.01). Pre-treatment with the inhibitors have no effect on anti-CD18 induced apoptosis.¶Conclusion: Anti-CD18 pansorbins induce apoptosis in neutrophils through an alternative pathway not involving reactive oxygen species and independent of tyrosine phosphorylation, cytoskeletal reorganisation and caspases.¶
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Received 1 March 2000; returned for revision 29 March 2000; accepted by I. Ahnfelt-Rønne 7 April 2000.
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Larsson, J., Serrander, L., Stendahl, O. et al. Involvement of the β 2-integrin CD18 in apoptosis signal transduction in human neutrophils. Inflamm res. 49, 452–459 (2000). https://doi.org/10.1007/s000110050616
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DOI: https://doi.org/10.1007/s000110050616