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Anti-inflammatory effects of vicenin-2 and scolymoside in vitro and in vivo

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Abstract

Aim and objective

Two structurally related flavonoids found in Cyclopia subternata, namely vicenin-2 and scolymoside, were examined for its effects on inflammatory responses by monitoring the effects of vicenin-2 and scolymoside on lipopolysaccharide (LPS)-mediated vascular inflammatory responses.

Methods

The anti-inflammatory activities of vicenin-2 and scolymoside were determined by measuring permeability, monocytes adhesion and migration, and activation of pro-inflammatory proteins in LPS-activated HUVECs and mice.

Results

We found that post-treatment of each compound inhibited LPS-induced barrier disruption, expression of cell adhesion molecules (CAMs), and adhesion/transendothelial migration of human neutrophils to human endothelial cells. Each compound induced potent inhibition of phorbol-12-myristate 13-acetate (PMA) and LPS-induced endothelial cell protein C receptor (EPCR) shedding. It also suppressed LPS-induced hyperpermeability and leukocytes migration in vivo. Furthermore, each compound suppressed the production of tumor necrosis factor-α (TNF-α) or Interleukin (IL)-6 and the activation of nuclear factor-κB (NF-κB) or extracellular regulated kinases (ERK) 1/2 by LPS. Moreover, post-treatment with each compound resulted in reduced LPS-induced lethal endotoxemia.

Conclusion

Vicenin-2 and scolymoside possess anti-inflammatory functions by inhibiting hyperpermeability, expression of CAMs, and adhesion and migration of leukocytes, thereby endorsing its usefulness as a therapy for vascular inflammatory diseases.

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Acknowledgments

This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT & Future Planning (NRF- 2012R1A5A2A42671316).

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    1. College of Pharmacy, CMRI, Research Institute of Pharmaceutical Sciences, Kyungpook National University, 80 Dahak-ro, Buk-gu, Daegu, 702-701, Republic of Korea

      Hyejin Kang, Byeongjin Jung & Jong-Sup Bae

    2. Department of Anatomy and Histology, College of Korean Medicine, Daegu Haany University, Gyeongsan, 712-715, Republic of Korea

      Sae-Kwang Ku

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    Responsible Editor: Liwu Li.

    H. Kang and S.-K. Ku contributed equally to this work.

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    Kang, H., Ku, SK., Jung, B. et al. Anti-inflammatory effects of vicenin-2 and scolymoside in vitro and in vivo. Inflamm. Res. 64, 1005–1021 (2015). https://doi.org/10.1007/s00011-015-0886-x

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