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Tumor necrosis factor-α, monocyte chemoattractant protein-1 and intercellular adhesion molecule-1 increase during the development of a 2,4-dinitrofluorobenzene-induced immediate-type dermatitis in rats

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Abstract

Background

Due to the steadily increasing incidence of atopic dermatitis, there is a great medical need for new therapies and improved animal models.

Objective

To provide more detailed analysis of a Sprague–Dawley rat dermatitis model.

Methods

Sprague–Dawley rats were actively sensitized by intraperitoneal injections of dinitrophenylated ovalbumin (DNP-OVA) plus alum. Skin reactions were elicited by repeated epicutaneous challenge with 2,4-dinitrofluorobenzene (DNFB).

Results

The ear thickness exhibited a significant increase from the first challenge. A relatively steep increase in ear thickness was observed at the fifth DNFB application. After the fifth DNFB application, total serum immunoglobulin (Ig) E and IgG1 levels reached a plateau at 1 h compared with the normal group. The peak production of tumor necrosis factor (TNF)-α and monocyte chemoattractant protein (MCP)-1 was found at 1 h, while that of intercellular adhesion molecule (ICAM)-1 was found at 24 h. Infiltration of CD4+ T cells, CD8+ T cells, eosinophils and mast cells increased in the skin lesion.

Conclusions

The indices such as thickness and inflammatory cell infiltration in the lesional skin were increased by repeated hapten application; TNF-α, MCP-1 and ICAM-1 increased with the development of the dermatitis.

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Acknowledgments

This work was supported by grants from the National Natural Science foundation of China (No. 81260462), Guangxi Education Department (Nos. 201012MS174 and 201203YB120), and Scientific Research Starting Foundation for Returned Overseas Chinese Scholars, Ministry of Education, China (No. 2011-508).

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Authors and Affiliations

  1. Pharmaceutical College, Guilin Medical University, No.109 North 2nd Huan cheng Road, Guilin, 541004, Guangxi, China

    Guangchen Sun

  2. Biotechnology College, Guilin Medical University, No.109 North 2nd Huan cheng Road, Guilin, 541004, Guangxi, China

    Yaqin Wang & Yingqin Liu

  3. Medical College, Jiamusi University, No. 148 Xuefu Street, Jiamusi, 154007, Heilongjiang, China

    Guangchen Sun, Bo Yin & Lingjin Zhu

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  1. Guangchen Sun
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  2. Yaqin Wang
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  3. Bo Yin
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  4. Lingjin Zhu
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  5. Yingqin Liu
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Corresponding author

Correspondence to Yingqin Liu.

Additional information

Responsible Editor: Michael Parnham.

Guangchen Sun and Wangyaqin contributed equally to this work.

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Sun, G., Wang, Y., Yin, B. et al. Tumor necrosis factor-α, monocyte chemoattractant protein-1 and intercellular adhesion molecule-1 increase during the development of a 2,4-dinitrofluorobenzene-induced immediate-type dermatitis in rats. Inflamm. Res. 62, 589–597 (2013). https://doi.org/10.1007/s00011-013-0611-6

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  • DOI: https://doi.org/10.1007/s00011-013-0611-6

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