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Gingival crevicular fluid IL-6, tPA, PAI-2, albumin levels following initial periodontal treatment in chronic periodontitis patients with or without type 2 diabetes

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Abstract

Objectives

To evaluate initial periodontal treatment effects on gingival crevicular fluid (GCF) interleukin-6 (IL-6), tissue-type plasminogen activator (tPA), plasminogen activator inhibitor-2 (PAI-2), albumin levels in type 2 diabetic patients.

Design and methods

GCF samples were collected from 20 type 2 diabetic, 22 non-diabetic non-smokers all with chronic periodontitis at baseline, 1-, 3-months following initial periodontal treatment. Biochemical analysis was performed by ELISA. Data were tested by Mann–Whitney U, Wilcoxon tests.

Results

The total amounts of albumin, IL-6, tPA, PAI-2 decreased significantly in diabetics after treatment (1- and 3-months) whereas, only PAI-2 decreased in non-diabetic group at 3-months (p < 0.05). There were statistically significant differences between the diabetics and non-diabetics at all time points for albumin, PAI-2 and at 1-, 3-months for GCF volume (p < 0.050) but only at baseline for IL-6 (p < 0.050).

Conclusion

Present data suggest clinical improvements are less apparent in diabetic chronic periodontitis patients as reflected by disease markers in GCF and by an increase in concentrations of inflammatory proteins IL-6, tPA, and PAI-2 in GCF of this patient group following initial periodontal treatment.

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References

  1. Mattson JS, Cerutis DR. Diabetes mellitus: a review of the literature and dental implications. Compend Contin Educ Den. 2001;22:757–60.

    CAS  Google Scholar 

  2. Soskolne WA, Klinger A. The relationship between periodontal diseases and diabetes: an overview. Ann Periodontol. 2001;6:91–8.

    Article  CAS  PubMed  Google Scholar 

  3. Lalla E, Lamster IB, Feit M, et al. Blockade of RAGE suppresses periodontitis-associated bone loss in diabetic mice. J Clin Invest. 2000;105:1117–24.

    Article  CAS  PubMed  Google Scholar 

  4. Taylor G, Burt B, Becker M, Genco RJ, Shlossman M. Glycemic control and alveolar bone loss progression in type 2 diabetes. Ann Periodontol. 1998;3:30–9.

    Article  CAS  PubMed  Google Scholar 

  5. Tsai C, Hayes C, Taylor GW. Glycemic control of type 2 diabetes and severe periodontal disease in US adult population. Community Dent Oral Epidemiol. 2002;30:182–92.

    Article  PubMed  Google Scholar 

  6. Mealey BL, Oates TW. Diabetes mellitus and periodontal diseases. J Periodontol. 2006;77:1289–303.

    Article  CAS  PubMed  Google Scholar 

  7. Emrich LJ, Schlossman M, Genco RJ. Periodontal disease in non-insulin dependent diabetes mellitus. J Periodontol. 1991;62:123–31.

    Article  CAS  PubMed  Google Scholar 

  8. Taylor G, Burt B, Becker M, et al. Non-insulin dependent diabetes mellitus and alveolar bone loss progression over 2 years. J Periodontol. 1998;69:76–83.

    CAS  PubMed  Google Scholar 

  9. American Diabetes Association. Diagnosis and classification of diabetes mellitus. Diabetes Care. 2007;30(suppl 1):s42–7.

    Article  Google Scholar 

  10. Ryan ME, Oana C, Kamer A. The influence of diabetes on the periodontal tissues. JADA. 2003;134:34S–40S.

    PubMed  Google Scholar 

  11. Eliasson MC, Jansson JH, Lindahl B, Stegmayr B. High levels of tissue plasminogen activator (tPA) antigen precede the development of type 2 diabetes in a longitudinal population study. The Northern Sweden MONICA Study. Cardio Diabet. 2003;22:2–19.

    Google Scholar 

  12. Astedt B, Hagerstrand I, Lecander I. Cellular localization in placenta of placental type plasminogen activator inhibitor. Thromb Haemost. 1986;56:63–5.

    CAS  PubMed  Google Scholar 

  13. Kruithof EKO, Baker MS, Bunn CL. Biological and clinical aspects of plasminogen activator inhibitor type 2. Blood. 1995;86:4007–24.

    CAS  PubMed  Google Scholar 

  14. Kinnby B, Matsson L, Astedt B. Aggravation of gingival inflammatory symptoms during pregnancy associated with the concentration of plasminogen activator inhibitor type 2 (PAI-2) in gingival fluid. J Periodont Res. 1996;31:271–7.

    Article  CAS  PubMed  Google Scholar 

  15. Xiao Y, Bunn CL, Bartold PM. Detection of tissue plasminogen activator (tPA) and plasminogen activator inhibitor 2 (PAI-2) in gingival crevicular fluid from healthy, gingivitis and periodontitis patients. J Clin Periodontol. 2000;27:149–56.

    Article  Google Scholar 

  16. Page RC, Eke PI. Case definitions for use in population-based surveillance of periodontitis. J Periodontol. 2007;78:1387–99.

    Article  PubMed  Google Scholar 

  17. Adonogianaki E, Mooney J, Wennstrom JL, Lekholm U, Kinane DF. Acute-phase proteins and immunoglobulin G against Porphyromonas gingivalis in peri-implant crevicular fluid: a comparison with gingival crevicular fluid. Clinical Oral Implants Research. 1995;6:14–23.

    Article  CAS  PubMed  Google Scholar 

  18. Shlossman M, Knowler WC, Pettitt DJ, Genco RJ. Type 2 diabetes mellitus and periodontal disease. J Am Dent Assoc. 1990;121:532–6.

    CAS  PubMed  Google Scholar 

  19. Christgau M, Palitzsch KD, Schmalz G, Kreiner U, Frenzel S. Healing response to non-surgical periodontal therapy in patients with diabetes mellitus: Clinical, microbiological, and immunological results. J Clin Periodontol. 1998;25:112–24.

    Article  CAS  PubMed  Google Scholar 

  20. Tervonen T, Knuuttila M, Pohjamo L, Nurkkala H. Immediate response to non-surgical periodontal treatment in subjects with diabetes mellitus. J Clin Periodontol. 1991;18:65–8.

    Article  CAS  PubMed  Google Scholar 

  21. Rodrigues DC, Taba MJ, Novaes AB, Souza SL, Grisi MF. Effect of non-surgical periodontal therapy on glycemic control in patients with type 2 diabetes mellitus. J Periodontol. 2003;74:1361–7.

    Article  PubMed  Google Scholar 

  22. Gonçalves D, Correa FO, Khalil NM, de Faria Oliveira OM, Orrico SR. The effect of non-surgical periodontal therapy on peroxidase activity in diabetic patients: a case-control pilot study. J Clin Periodontol. 2008;79:2143–50.

    Article  Google Scholar 

  23. Correa FOB, Gonçalves D, Figueredo CMS, Gustafsson A, Orrico SRP. The short-term effectiveness of non-surgical treatment in reducing levels of interleukin-1β and proteases in gingival crevicular fluid from type 2 diabetes patients with chronic periodontitis. J Periodontol. 2008;79:2143–50.

    Article  CAS  PubMed  Google Scholar 

  24. O’Connell PA, Taba M, Nomizo A, Foss Freitas MC, Suaid FA, Uyemura SA, Trevisan GL, Novaes AB, Souza SL, Palioto DB, Grisi MF. Effects of periodontal therapy on glycemic control and inflammatory markers. J Periodontol. 2008;79:774–83.

    Article  PubMed  Google Scholar 

  25. Da Cruz GA, de Toledo S, Sallum EA, Sallum AW, Ambrosano GM, de Cássia OSJ, da Cruz SE, Gonçalves RB. Clinical and laboratory evaluations of non-surgical periodontal treatment in subjects with diabetes mellitus. J Periodontol. 2008;79:1150–7.

    Article  PubMed  Google Scholar 

  26. Kardeşler L, Buduneli N, Çetinkalp Ş, Kinane DF. Adipokines and inflammatory mediators following initial periodontal treatment in type 2 diabetic chronic periodontitis patients. J Periodontol. 2010;81:24–33.

    Article  PubMed  Google Scholar 

  27. Kıran M, Arpak N, Ünsal E, Erdoğan MF. The effect of improved periodontal health on metabolic control in type 2 diabetes mellitus. J Clin Periodontol. 2005;32:266–72.

    Article  PubMed  Google Scholar 

  28. Syrjälä AM, Ylöstalo P, Niskanen MC, Knuuttila ML. Role of smoking and HbA1c level in periodontitis among insulin-dependent diabetic patients. J Clin Periodontol. 2003;30:871–5.

    Google Scholar 

  29. Navarro-Sanchez AB, Faria-Almeida R, Bascones-Martinez A. Effect of non-surgical periodontal therapy on clinical and immunological response and glycaemic control in type 2 diabetic patients with moderate periodontitis. J Clin Periodontol. 2007;34:835–43.

    Article  PubMed  Google Scholar 

  30. Lamster IB, Oshrain RL, Gordon JM. Enzyme activity in human gingival crevicular fluid: considerations in data reporting based on analysis of individual crevicular sites. J Clin Periodontol. 1986;13:799–804.

    Article  CAS  PubMed  Google Scholar 

  31. Tsai CC, Ho YP, Chen CC. Levels of interleukin-1 beta and interleukin-8 in gingival crevicular fluids in adult periodontitis. J Periodontol. 1995;66:852–9.

    CAS  PubMed  Google Scholar 

  32. Kurtiş B, Develioğlu H, Taner IL, Baloş K, Tekin IO. IL-6 levels in gingival crevicular fluid (GCF) from patients with non-insulin dependent diabetes mellitus (NIDDM), adult periodontitis and healthy subjects. J Oral Sci. 1999;41:163–7.

    PubMed  Google Scholar 

  33. Kardeşler L, Buduneli N, Bıyıkoğlu B, Çetinkalp Ş, Kütükçüler N. Gingival crevicular fluid PGE2, IL-1beta, t-PA, PAI-2 levels in type 2 diabetes and relationship with periodontal disease. Clin Biochem. 2008;41:863–8.

    Article  PubMed  Google Scholar 

  34. Kardeşler L, Bıyıkoğlu B, Çetinkalp Ş, Pitkala M, Sorsa T, Buduneli N. Crevicular fluid matrix metalloproteinase-8, -13, and TIMP-1 levels in type 2 diabetics. Oral Dis. 2010;16:476–81.

    Article  PubMed  Google Scholar 

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Acknowledgments

This study was supported by the grant from the Ege University Research Foundation (2005 Diş 015), İzmir, Turkey, also by University of Lousville, KY, USA and University of Glasgow, Glasgow, UK. The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Department of Periodontology, School of Dentistry, Ege University, 35100, Bornova, İzmir, Turkey

    Levent Kardeşler & Nurcan Buduneli

  2. Department of Metabolic Diseases and Endocrinology, School of Medicine, Ege University, İzmir, Turkey

    Şevki Çetinkalp

  3. Infection and Immunity Group, Glasgow Dental School, School of Medicine, University of Glasgow, Glasgow, UK

    David Lappin

  4. Department of Periodontology, School of Dentistry, University of Louisville, Louisville, KY, USA

    Denis F. Kinane

Authors
  1. Levent Kardeşler
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  2. Nurcan Buduneli
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  3. Şevki Çetinkalp
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  4. David Lappin
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  5. Denis F. Kinane
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Corresponding author

Correspondence to Nurcan Buduneli.

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Responsible Editor: Claudia Kasserra.

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Kardeşler, L., Buduneli, N., Çetinkalp, Ş. et al. Gingival crevicular fluid IL-6, tPA, PAI-2, albumin levels following initial periodontal treatment in chronic periodontitis patients with or without type 2 diabetes. Inflamm. Res. 60, 143–151 (2011). https://doi.org/10.1007/s00011-010-0248-7

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  • DOI: https://doi.org/10.1007/s00011-010-0248-7

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