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An anti-inflammatory lectin from Luetzelburgia auriculata seeds inhibits adhesion and rolling of leukocytes and modulates histamine and PGE2 action in acute inflammation models

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Abstract

Objectives

To study and characterize the in vivo effect of the lectin from Luetzelburgia auriculata seed on acute inflammation models.

Methods

The lectin was purified from the crude saline extract by affinity chromatography on a guar-gum matrix. Native, heat-treated, and digested lectin was evaluated for anti-inflammatory activity by using peritonitis and paw edema models. The anti-inflammatory activity was characterized by intravital microscopy, nitric oxide production, and myeloperoxidase activity.

Results

The lectin exhibited anti-inflammatory activity (2 mg/kg) on both models, reducing local myeloperoxidase activity. Galactose or heat treatment (100°C, 10 min) reduced anti-inflammatory action. Anti-inflammation involves the inhibition of adhesion and rolling of leukocytes along with augmentation of nitric oxide in serum. The lectin inhibited the edematogenic effect of histamine and prostaglandins (PGE2) but did not alter the chemoattractant effect of IL-8.

Conclusions

The results indicate that this lectin is a potent anti-inflammatory molecule. Its effects engage diverse modulatory events.

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Acknowledgments

To International Foundation for Science (Grant No. IFS 3070-2, M.V.R.); Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq). The authors are in debt to Mr. Brian Stephen Currey who critically reviewed the language of the manuscript.

Author information

Authors and Affiliations

  1. Departamento de Fisiologia e Farmacologia, Universidade Federal do Ceará, Cel. Nunes de Melo 1127, Fortaleza, CE, CEP 60430-270, Brazil

    N. M. N. Alencar & I. J. M. Cavalcante

  2. Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Caixa Postal 6033, Fortaleza, CE, CEP 60451-970, Brazil

    R. S. B. Oliveira, J. G. Figueiredo, M. P. V. Matos, J. V. S. Nunes & M. V. Ramos

  3. Departamento de Farmacologia, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Av Bandeirantes 3900, Ribeirão Preto, SP, Brazil

    F. Q. Cunha

  4. Faculdade de Educação de Crateús, FAEC, Universidade Estadual do Ceará, Crateús, CE, 63700-000, Brazil

    L. R. Bomfim

Authors
  1. N. M. N. Alencar
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  2. R. S. B. Oliveira
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  3. J. G. Figueiredo
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  4. I. J. M. Cavalcante
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  5. M. P. V. Matos
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  6. F. Q. Cunha
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  7. J. V. S. Nunes
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  8. L. R. Bomfim
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  9. M. V. Ramos
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Corresponding authors

Correspondence to N. M. N. Alencar or M. V. Ramos.

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Responsible Editor: J. Skotnicki.

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Alencar, N.M.N., Oliveira, R.S.B., Figueiredo, J.G. et al. An anti-inflammatory lectin from Luetzelburgia auriculata seeds inhibits adhesion and rolling of leukocytes and modulates histamine and PGE2 action in acute inflammation models. Inflamm. Res. 59, 245–254 (2010). https://doi.org/10.1007/s00011-009-0092-9

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  • DOI: https://doi.org/10.1007/s00011-009-0092-9

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