Abstract.
Objective: This study evaluates the poly inosinic acid (poly I)-induced activation in the murine monocytemacrophage cell line RAW 264.7, which led to an inflammatory phenotype.
Material: RAW 264.7, and WEHI 164 cell lines were used.
Results: The activation process is characterized by the acquisition of a mature macrophage morphology and the production of inflammatory mediators tumor necrosis factor (TNF) and nitric oxide (NO). The activation by poly I has distinctive features. Thus, poly I induced an increase in nuclear factor κB (NF-κB) transcriptional activity due to a long-term degradation of inhibitory NF-κB (IκB) β while lipopolysaccharide (LPS) induced the degradation of both IκBα and IκBβ. Poly I also induced an increase in activator protein 1 (AP-1) transcriptional activity, possibly due to the activation of the mitogen activated protein kinases (MAPKs) ERK, Jun N terminal kinase (JNK) and p38. Dextran sulphate (DS) efficiently inhibited the activation induced by poly I including the production of the inflammatory mediators. Dextran sulphate also inhibited AP-1 and NF-κB transcriptional activities in poly I-stimulated cells. RAW 264.7 cells express macrophage scavenger receptor 1 (Msr1) type I and Msr1 type II that are differently up-regulated upon treatment with poly I.
Conclusions: The results presented demonstrate that the well-known blocker of scavenger receptors poly I activates macrophages to produce TNF and NO, triggering specific signal transduction pathways.
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Received 26 May 2004; returned for revision 23 September 2004; accepted by I. Ahnfelt-Rønne 20 April 2005
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Campa, V.M., Iglesias, J.M., Carcedo, M.T. et al. Polyinosinic acid induces TNF and NO production as well as NF-κB and AP-1 transcriptional activation in the monocytemacrophage cell line RAW 264.7. Inflamm. res. 54, 328–337 (2005). https://doi.org/10.1007/s00011-005-1359-4
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DOI: https://doi.org/10.1007/s00011-005-1359-4