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CD40-activated B cells from patients with systemic lupus erythematosus can be modulated by therapeutic immunoglobulins in vitro

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Archivum Immunologiae et Therapiae Experimentalis Aims and scope

Abstract

Introduction

Aberrant signaling within and between B and T cells, considered to be central in systemic lupus erythematosus (SLE), could depend on enhanced CD40-CD154 activation. As a result, autoreactive B cells, normally anergic, differentiate and secrete antibodies attacking several normal tissues. Thus restorating B cell homeostasis might help control this disease. In this study, two facets of SLE B cells were investigated, namely their in vitro response to CD40-CD154 and the effect of treatment with human immunoglobulins for intravenous use (IVIg).

Materials and Methods

Blood samples from SLE patients and healthy volunteers were obtained and used to isolate B cells, which were activated through CD40 in the presence or absence of IVIg. The phenotype, proliferation, and differentiation of the SLE B cells were determined and compared with those of control B cells using flow cytometry and standard ELISA.

Results

In this model, CD40-activated SLE B cells, as control B cells, proliferated and differentiated and were characterized by the emergence of CD19loCD38++CD138+CD27++ cells. IVIg treatment of the CD40-activated SLE B cells resulted in higher differentiation, characterized by increased secretion rates of IgG and IgM, as reported previously for control B cells.

Conclusions

Taken as a whole, such accelerated differentiation of CD40-activated B cells suggests that IVIg may participate in re-equilibration of the antibody repertoire by replacing pathological antibodies by de novo harmless antibodies.

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Authors and Affiliations

  1. Recherche et développement, Héma-Québec, Ingénierie cellulaire, 1070, avenue des Sciences-de-la-vie, Québec, Qc, G1V 5C3, Canada

    Sonia Néron Ph.D., Nathalie Dussault, Claudia Racine, Serge Côté & Annie Jacques

  2. Département de biochimie et microbiologie, Faculté des sciences et de génie, Université Laval, Québec, Québec, Canada

    Sonia Néron Ph.D. & Serge Côté

  3. Service de rhumatologie, Faculté de médecine et des sciences de la santé, Université de Sherbrookeet Centre hospitalier universitaire de Sherbrooke, Sherbrooke, Québec, Canada

    Gilles Boire & Artur J. de Brum-Fernandes

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  1. Sonia Néron Ph.D.
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  2. Gilles Boire
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  3. Nathalie Dussault
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Correspondence to Sonia Néron Ph.D..

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Néron, S., Boire, G., Dussault, N. et al. CD40-activated B cells from patients with systemic lupus erythematosus can be modulated by therapeutic immunoglobulins in vitro . Arch. Immunol. Ther. Exp. 57, 447–458 (2009). https://doi.org/10.1007/s00005-009-0048-3

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  • DOI: https://doi.org/10.1007/s00005-009-0048-3

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