Skip to main content

Advertisement

Log in

Increased whole blood chemiluminescence in patients with chronic renal failure independent of hemodialysis treatment

  • Published:
Archivum Immunologiae et Therapiae Experimentalis Aims and scope

Abstract.

Introduction:

The luminol-enhanced whole blood chemiluminescence (LBCL) assay is a rapid assay for the measurement of reactive oxygen species (ROS) generation by circulating phagocytes. This study’s aim was to determine if patients on maintenance hemodialysis (HD) and non-dialyzed patients with chronic renal failure (CRF) have altered LBCL and if dialysis itself affects ROS production in the blood.

Materials and Methods:

Twenty-six HD patients, 11 non-dialyzed patients with CRF, and 20 gender- and age-matched healthy controls were studied. Resting (rCl) and 2 ×  10−5 M n-formyl-methionyl-leucyl-phenylalanine-stimulated LBCL (peak chemiluminescence: pCl, total light emission after agonist addition: tCl) calculated per 104 phagocytes present in the 3-μl blood samples were measured with a Bio-Orbit® 1251 luminometer at 37°C for 11 min.

Results:

Prior to the HD session, median rCL, pCL, and tCL were 1.5, 3.0, and 2.8 times higher in HD patients than in healthy controls (p<0.01) and tended to increase at the end of the session. Significant increases in tCl were observed at 30 min and 240 min (end) of HD (1023.5 vs. 1810.6 vs. 2006.8 arbitrary units ×  s/104 phagocytes, n=9, p<0.05). Median pCl and tCl were 5.0 and 4.3 times higher in non-dialyzed patients with CRF than in healthy controls (p<0.001). However, no significant differences were found between pre- and post-HD LBCL of HD patients and the LBCL of non-dialyzed patients with renal failure.

Conclusions:

Blood from patients with renal failure generates elevated amounts of oxidants independently of HD treatment. This may add to the understanding of the nature of oxidative stress and suggests the need of anti-oxidant treatment in these patients.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Dariusz Nowak.

About this article

Cite this article

Rysz, J., Potargowicz, E., Banach, M. et al. Increased whole blood chemiluminescence in patients with chronic renal failure independent of hemodialysis treatment. Arch. Immunol. Ther. Exp. 54, 347–355 (2006). https://doi.org/10.1007/s00005-006-0040-0

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00005-006-0040-0

Keywords.

Navigation