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Endothelial function and endothelial nitric oxide synthase intron 4a/b polymorphism in primary hyperparathyroidism

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Abstract

Background and aim: Patients with symptomatic primary hyperparathyroidism (pHT) have increased cardiovascular morbidity and mortality. Endothelial nitric oxide synthase (eNOS) intron 4a/b polymorphism is associated with coronary artery disease and hypertension in various populations. Our aim is to evaluate endothelial function in patients with pHT during pre-operative hypercalcemic and post-operative normocalcemic periods and to determine whether intron 4a/b polymorphism of eNOS gene influences endothelial function. Subjects and Methods: Forty patients with pHT (age 48.48±11.64 yr) were examined pre-operatively and reexamined 5.8±1.9 months after parathyroidectomy. Forty-three healthy subjects (age 47.13±8.14 yr) were served as control group. Endothelial function was determined by flow-mediated dilation of brachial artery (FMD). eNOS4a/b polymorphism was detected by polymerase chain reaction. Results: FMD was significantly lower in patients pre-operatively compared with controls (8.48±1.78% vs 19.49±2.34%, p<0.001). FMD improved significantly after parathyroidectomy (16.19±2.16%, p<0.001 compared with pre-operative measurements), but was still significantly lower than controls (p<0.001). The distribution of eNOS4a/b genotype frequencies was not significantly different between patients and controls. Logistic regression analysis showed that increased serum calcium (>2.47 mmol/l) and PTH concentrations (>7.75 pmol/l) were significant independent predictors of lower FMD (<16.7%). ENOS4a/b polymorphism did not enter in this model. Conclusion: Impaired endothelial function in patients with pHT improves after successful parathyroid surgery. No compelling data are evident to suggest that eNOS4a/b polymorphism modifies the endothelial function in patients with pHT.

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Authors and Affiliations

  1. Department of Medicine, Division of Endocrinology, Istanbul Faculty of Medicine, 34390, Capa, Istanbul, Turkey

    A. Ekmekci

  2. Department of Genetics, Institute for Experimental Medical Research, Turkey

    N. Abaci & N. Erginel-Unaltuna

  3. Division of Endocrinology, Istanbul University, Istanbul, Turkey

    N. Colak Ozbey

  4. Department of Radiology, Istanbul University, Istanbul, Turkey

    A. Agayev

  5. Department of Surgery, Istanbul University, Istanbul, Turkey

    N. Aksakal & Y. Erbil

  6. Department of Cardiology, Istanbul Medical Faculty, Istanbul University, Istanbul, Turkey

    H. Oflaz

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  1. A. Ekmekci
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  2. N. Abaci
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  3. N. Colak Ozbey
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Correspondence to N. Colak Ozbey.

Additional information

This work has been presented — in part — as an oral presentation in 10th European Congress of Endocrinology, 3–7 May, 2008, Berlin, Germany (Abstract Book S30.4).

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Ekmekci, A., Abaci, N., Ozbey, N.C. et al. Endothelial function and endothelial nitric oxide synthase intron 4a/b polymorphism in primary hyperparathyroidism. J Endocrinol Invest 32, 611–616 (2009). https://doi.org/10.1007/BF03346518

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