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A Histological Analysis of Regeneration in Watermelon

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Abstract

The optimization of regeneration protocol for different genotypes increases the yield in transformation studies. Cotyledon explants of watermelon [Citrullus lanatus (Thunb) Matsum & Nakai] cv Crimson Sweet were cultured on MS medium containing combinations of benzyl adenine (BA) (0, 5, 10, 20 µM) and indole-3-acetic acid (IAA) (0, 0.5, 5 µM). Maximum shoot growth and subsequent rooting from explants on regeneration medium were obtained from the media containing 10 µM BA + 0.5 µM IAA and 20 µM BA (75 and 78%) by direct organogenesis, respectively. Histological analysis showed that cell division was observed in the epidermal and subepidermal layers. Protuberant structures were observed in tissues between 7 and 12 days in culture. Meristematic structures were observed after 12days in culture which later developed into buds.

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Authors and Affiliations

  1. Department of Horticulture, Faculty of Agriculture, University of Cukurova, 01330, Balcali, Adana, Turkey

    N. Y. Yalcin-Mendia, H. Kacan & N. Sari

  2. Department of Horticulture, Faculty of Agriculture, University of Mustafa Kemal, 31034, Antakya, Turkey

    S. Curuk

  3. Faculty of Engineering and Natural Sciences, University of Sabanci, 81474, Tuzla, Istanbul, Turkey

    S. Cetiner

  4. Department of Virology, ARO Volcani Center, POB 6, Bet Dagan, 50250, Israel

    V. Gaba

Authors
  1. N. Y. Yalcin-Mendia
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  2. M. Ipek
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  3. H. Kacan
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  4. S. Curuk
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  5. N. Sari
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  6. S. Cetiner
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  7. V. Gaba
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Correspondence to N. Y. Yalcin-Mendia.

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Yalcin-Mendia, N.Y., Ipek, M., Kacan, H. et al. A Histological Analysis of Regeneration in Watermelon. J. Plant Biochem. Biotechnol. 12, 147–150 (2003). https://doi.org/10.1007/BF03263176

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  • DOI: https://doi.org/10.1007/BF03263176

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