Abstract
The ability of theRhizobium ciceri, to produce extracellular polysaccharides (EPS) was investigated. Tested carbon and nitrogen sources influced EPS synthesis whenR. ciceri Rc5 was grown in a chemically defined medium. Mannitol was the most efficient carbon source among the six sources tested (sucrose, glucose, arabinose, fructose, xylose and rhamnose) and sodium nitrate was the most efficient nitrogen source among the two tested (ammonium sulphate and glycine). High amounts of EPS (1182.0 and 1015 μg ml−1, respectively) were produced by the Rc5 strain in mannitol and sodium nitrate respectively, which was accompanied by a great increase in the production compared to the control.
References
Bassio J.C., Semino N., Inon de Iannino M.A., Dankert M.A. (1996). Thein vitro biosynthesis of the exopolysaccharide production byRhizobium leguminosarum bv.trifolii, strain NA 30. Cell Mol. Biol., 42: 737–758.
Battisti L., Lara J.C., Leigh J.A. (1992). Specific oligosaccharide form of theRhizobium meliloti exopolysaccharide promotes nodule invasion in alfalfa. Proc. Natl. Acad. Sci. USA, 89: 5625–5629.
Becker A., Pühler A. (1998). Production of exopolysaccharides, In: Spaink H.P., Kondorosi A., Hooykaas P.J.J., Eds, The Rhizobiaceae: Molecular Biology of Model Plant Associated Bacteria, Kluwer Academic Publishers, Dordrecht, The Netherlands, pp. 97–118.
Breedveld M.W., Zevenhevizen L.P.T., Conter-Cremes H.C.J., Zehnder A.J.B. (1993). Influence of growth conditions on production of capsular and extracellular polysaccharides byRhizobium leguminosarum. Ant. Leeuw. J Microbiol., 64: 1–8.
Cunningham S.D., Munns D.N. (1984). The correlation of the exopolysaccharide production and acid tolerance inRhizobium. Soil Sci. Soc. Am. J., 48: 1273–1276.
Datta C., Basu P.S. (1999). Production of extracellular polysaccharides by aRhizobium species from root nodules ofCajanus cajan. Acta Biotechnol., 19: 59–68.
Dudman W.F. (1964). Growth and extracellular polysaccharide production byRhizobium meliloti in defined medium. J. Bacteriol., 88: 640–645.
Duta P.F., Da Costa A.C.A., Lopes L.M.A., Barros A., Servulo E.F.C., de Franca F.R. (2004). Effect of process parameters on production of a biopolymer byRhizobium sp. Appl. Biochem. Biotechnol. 114: 639–652.
Duta P.F., Pesson de Franca F., Almeida Lopes L.M. (2006). Optimization of culture conditions for exopolysaccharides production inRhizobium sp. using the response surface method. Elec. J. Biotechnol., 9 (4): 391–399.
Düzgüneş O., Kesici T., Kavuncu O., Gürbüz F. (1987). Research and Experiment Methods. Pub. no. 295. Ankara Univ. Faculty of Agriculture, Ankara, Turkey.
Ghosh A.C., Ghosh S, Basu P.S. (2005). Production of extracellular polysaccharide by aRhizobium species from root nodules of the leguminous treeDalbergia lanceolaria. Eng. Life Sci., 5: 378–382.
Hirsch A.M. (1999). Role of lectins (and rhizobial exopolysaccharides) in legume nodulation. Curr. Op. Plant Biol., 2: 320–326.
Jarman T.R., Deuvin L., Slocombe S., Righelato R.C. (1978). Investigation of the effect of environmental conditions on the rate of exopolysaccharide synthesis inAzotobacter vinelandii. J. Gen. Microbiol., 107: 59–64.
Kondorosi S.M. (1998). Regulation of symbiotic root nodule development. Annu. Rev. Genet., 32: 33–57.
Leigh J.A., Coplin D.L. (1992). Exopolysaccharides in plant bacterial interactions. Annu. Rev. Microbiol., 46: 307–346.
Nandal K., Schrawat A.R., Yadav A.S., Vashishat R.K., Bora K.S. (2005). High temperature induced changes in exopolysaccharides, lipopolysaccharides and protein profile of heat resistant mutant ofRhizobium sp. (Cajanus sp.). Microbiol. Res., 1: 1–7.
Putnoky P., Petrovics G., Kereszt A., Grosskopf E., Cam Ha D., Banfalvi Z., Kondorosi A. (1990).Rhizobium meliloti lipopolysaccharide and exopolysaccharide can have the same function in the plant-bacterium interaction. J. Bacteriol., 172: 5450–5458.
Vermani M.V., Kelkar S.M., Kamat M.Y. (1995). Novel exopolysaccharide production byAzotobacter vinelandii isolated from plant rhizosphere. Biotechnol. Lett., 17: 917–920.
Zevenhuizen L.P.T.M. (1986). Selective synthesis of polysaccharides byRhizobium trifolii, strain TA-1. FEMS Microbiol. Lett., 35: 43–47.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Küçük, Ç., Kivanç, M. Extracellular polysaccharide production byRhizobium ciceri from Turkey. Ann. Microbiol. 59, 141–144 (2009). https://doi.org/10.1007/BF03175611
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF03175611