ABSTRACT
Verticillium wilt is an economically important disease that causes reduced tuber yields in potato. Pathogen population dynamics were studied during disease development following combined inoculations of potato withV. albo-atrum and various otherVerticillium species. In greenhouse and field studies, threeVerticillium species were examined:V. albo-atrum ‘group 1’,V albo-atrum ‘group 2’ andV tricorpus. Potato plants were inoculated with two out of the three species in various combinations of an aggressive (V albo-atrum ‘group 1’) and weak (V. albo-atrum ‘group 2’ orV. tricorpus) pathogen. Initial inoculations occurred on the same date or with a weak species followed by an aggressive species four days later. Plant and soil samples were collected and relative population levels (RPLs) of each pathogen were determined using polymerase chain reaction (PCR) techniques. In combinations where pathogens were inoculated at the same time, RPLs of the weaker species did not exceed those of the aggressive species. In combinations where the weaker species were inoculated first, followed by the aggressive species four days later, the two weaker species were able to exceed RPLs and reduce visual wilt symptoms ofV. albo-atrum ‘group 1’. Implications of these findings on epidemiological aspects of these host-pathogen interactions are discussed.
Resumen
La marchitez porVerticillium es una enfermedad económicamente importante que causa reducción del rendimiento de tubérculos de papa. La dinámica poblacional del patógeno fue estudiada durante el desarrollo de la enfermedad, después de una inoculación combinada conV. albo-atrum y otras especies deVerticillium. En estudios de invernadero y campo, se examinaron tres especies deVerticillium: V albo-atrum ‘grupo 1’,V. alboatrum ‘grupo 2’ yV tricorpus. Las plantas de papa fueron inoculadas con dos de las tres especies en varias combinaciones de un patógeno agresivo (V. albo-atrum ‘grupo 1’) y uno débil (V. albo-atrum ‘grupo 2’ oV. tricorpus). Las inoculaciones iniciales se realizaron en la misma fecha o con una especie débil seguidas de una especie agresiva cuatro días después. Se colectaron muestras de plantas y de suelo infectado y se determinaron los niveles de población relativa (NsPR) de cada patógeno utilizando la técnica de reacción en cadena de la polimerasa (PCR). En combinaciones en las que los patógenos fueron inoculados al mismo tiempo, los NsPR de las especies débiles no excedieron a aquellas de las especies agresivas. En combinaciones en las en las que las especies débiles fueron inoculadas primero seguidas de inoculaciones con especies agresivas cuatro días después, las dos especies débiles excedieron los NsPR y redujeron los síntomas visuales deV. albo-atrum ‘grupo 1’. Se discuten las implicancias de estos hallazgos sobre aspectos epidemiólogicos de esta interacción huéspedpatógeno.
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Robinson, N., Platt, H.W.(. & Hale, L.R. Interactions of variousverticillium species in combination withV. albo-atrum on verticillium wilt disease development in potato. Amer J of Potato Res 84, 133–141 (2007). https://doi.org/10.1007/BF02987136
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DOI: https://doi.org/10.1007/BF02987136