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The effects of amifostine and dexamethasone on brain tissue lipid peroxidation during oxygen treatment of carbon monoxide-poisoned rats

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Abstract

The mechanisms of injury of, and methods of treating patients with, carbon monoxide (CO) poisoning are poorly understood. Besides the hypoxic degenerative effects of CO, reoxygenation injury may play an important role. Amifostine (Ami), which is most often used in radiotherapy for its tissue protective characteristics, may offer benefits. In this study, investigators evaluated the effectiveness of various treatments in a CO-poisoned rat model. A total of 36 Wistar rats were randomly assigned to 1 of 6 groups (n=6 each), including control and poisoned groups exposed to CO at 2000 ppm (v/v) for 1 h, followed by various 1-h treatments: group C (control), group CO-air (ambient air), group CO-NBO (normobaric 100% oxygen), group CO-HBO (hyperbaric oxygen with 3 atmospheres absolute [3 ATA]), group CO-NBO-Ami (normobaric oxygen with intraperitoneal [IP] injection of amifostine 250 mg/kg body weight [bw]), and group CO-70O (70% O2 and 5% CO2 with dexamethasone 10 mg/kg bw, IP). Blood gas analysis, carboxy-hemoglobin determination, brain tissue lipid peroxidation, and glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), lactate dehydrogenase (LDH), and creatine kinase (CK) activities were evaluated. Carboxyhemoglobin concentration in the air-treated group was 44±2%; it decreased to the control level with all oxygen treatments. Brain tissue GSH-Px and SOD measurements did not change. The activity of LDH in group CO-HBO and the activities of LDH and CKin group CO-70O were similar to those of group C. Lipid peroxides were high in ambient air and normobaric oxygen, but HBO, amifostine with oxygen, or 70% O2 reduced these to control levels (P < .05).

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Authors and Affiliations

  1. School of Medicine, University of Pamukkale, Bayramyeri-Denizli, Turkey

    Habip Atalay (Assist Prof)

  2. Department of Biochemistry, University of Pamukkale School of Medicine, Turkey

    Hülya Aybek (Assist Prof)

  3. Department of Biochemistry, Ataturk State Hospital, Izmir, Turkey

    Mehmet Koseoglu (Assoc Prof)

  4. Department of Biochemistry, Turkey

    Suleyman Demir (Assoc Prof)

  5. Department of Anesthesiology and Reanimation, University of Pamukkale School of Medicine, Denizli, Turkey

    Hakan Erbay (Assoc Prof)

  6. Department of Internal Medicine, University of Adnan Menderes School of Medicine, Aydin, Turkey

    Ali Zahit Bolaman (Prof)

  7. Department of Biochemistry, University of Ankara School of Medicine, Ankara, Turkey

    Aslihan Avci (Assist Prof)

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  1. Habip Atalay
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  2. Hülya Aybek
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  4. Suleyman Demir
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  6. Ali Zahit Bolaman
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  7. Aslihan Avci
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Atalay, H., Aybek, H., Koseoglu, M. et al. The effects of amifostine and dexamethasone on brain tissue lipid peroxidation during oxygen treatment of carbon monoxide-poisoned rats. Adv Therapy 23, 332–341 (2006). https://doi.org/10.1007/BF02850138

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