Abstract
The process of cooperative binding of ligands to DNA has been classified into different modes. An additional mode of cooperative interaction amongst ligands binding at sites on complementary strands has been emphasised. A statistical mechanical method has been applied to obtain an analytical expression for the fraction of nucleotide sites bound. Theoretical Scatchard plots have been drawn and analysed.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Literature
Alberts, B. M., F. J. Amodio, M. Jenkins, E. D. Gutmann and F. L. Ferris. 1968. “Studies with DNA-Cellulose Chromatography. I. DNA-binding Protein fromE. coli.”Cold Spring Harbor Symp. Quant. Biol. 33, 289–305.
Bloomfield, V. A., D. M. Crothers and I. Tinoco, Jr. 1974.Physical Chemistry of Nucleic Acids. Chap. 7. New York: Harper and Row.
Bradley, D. F. and M. K. Wolf. 1959. “Aggregation of Acridine Dyes on Native and Denatured Deoxyribonucleates.”Proc. natn. Acad. Sci. U.S.A 45, 944–952.
Cary, P. D., T. Moss and E. M. Bradbury. 1978. “High Resolution Proton Magnetic Resonance Studies of Chromatin Core Particles.”Eur. J. Biochem. 89, 475–482.
Chadwick, P., V. Pirrota, R. Steinberg, N. Hopkins and M. Ptashne. 1970. “The λ and 434 phage repressors.”Cold Spring Harbor Symp. Quant. Biol. 35, 283–294.
Dougherty, G. and W. J. Pigram. 1982. “Spectroscopic Analysis of Drug-Nucleic Acid Interactions.”CRC Critical Reviews in Biochemistry, February, pp. 103–132.
Dourlent, M. 1975. “Competttive Cooperative Binding of Small Ligands to a Linear Homopolymer. I. Extension of the Ising Model to the Case of Two Competitive Interactions.”Biopolymers 14, 1717–1738.
Epstein, I. R. 1978. “Cooperative and Non-cooperative Binding of Large Ligands to a Finite One-dimensional Lattice. A Model for Ligand-Oligonucleotide Interaction.”Biophys. Chem. 8, 327–339.
McGhee, J. D. and P. H. von Hippel. 1974. “Theoretical Aspects of DNA-Protein Interactions: Cooperative and Non-cooperative Binding of Large Ligands to a One-dimensional Homogeneous Lattice.”J. molec. Biol. 86, 469–489.
Mukhopadhyay, R. and A. Mookerjee. 1981. “Effect of Gamma-irradiation on Dye-DNA Binding.”Int. J. Radiat. Biol. 39, 143–155.
Peacocke, A. R. 1975. “The Interaction of Acridines with Nucleic Acids.” InAcridines (Ed. R. M. Acheson), 2nd edn, pp. 723–756. New York: Wiley Interscience.
Schwarz, G. 1970. “Cooperative Binding to Linear Bipolymers I. Fundamental Static and Dynamic Properties.”Eur. J. Biochem. 12, 442–453.
Shick, V. V., A. V. Belyavsky, S. G. Bavykin and A. D. Mirzabekov. 1980. “Primary Organisation of the Nucleosome Core Particles: 1. Sequential Arrangement of Histones Along DNA.”J. molec. Biol. 139, 491–517.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Ghosh, S., Mookerjee, A. A model for cooperative ligand binding at complementary sites of DNA. Bltn Mathcal Biology 48, 21–27 (1986). https://doi.org/10.1007/BF02460060
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF02460060