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Proton magnetic resonance spectroscopy of vitreal changes in experimental streptococcal endophthalmitis

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Abstract

We used magnetic resonance spectroscopy to examine endophthalmitis in rabbits inoculated with a virulent strain ofStreptococcus pneumoniae. On different days after infection, the animals were sacrificed and the vitreous isolated and examined with water-suppressed proton magnetic resonance spectroscopy. A broad resonance corresponding to the methyl envelope of lipoprotein lipids appeared 2 days after infection and persisted until the eyes developed phthisis (around 10 days postinfection). This resonance was absent in the control eye and the bacterial culture; it could be used as the marker of breakdown of blood-vitreous barrier and onset of endophthalmitis-induced changes.

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Authors and Affiliations

  1. Howe Laboratory of Ophthalmology, Harvard Medical School, and Massachusetts Eye and Ear Infirmary, 243 Charles Street, 02114, Boston, MA, USA

    Hong-Ming Cheng

  2. Schepens Eye Research Institute, 20 Staniford Street, 02114, Boston, MA, USA

    Mark S. Hughes, Kameran Lashkari, Delia Sang, Akitoshi Yoshida & J. Wallace MCMeel

  3. Infectious Unit, Massachusetts General Hospital, Fruit Street, 02114, Boston, MA, USA

    Ann S. Baker

Authors
  1. Hong-Ming Cheng
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  2. Mark S. Hughes
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  3. Kameran Lashkari
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  4. Delia Sang
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  5. Akitoshi Yoshida
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  6. J. Wallace MCMeel
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  7. Ann S. Baker
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Cheng, HM., Hughes, M.S., Lashkari, K. et al. Proton magnetic resonance spectroscopy of vitreal changes in experimental streptococcal endophthalmitis. Graefe's Arch Clin Exp Ophthalmol 231, 402–404 (1993). https://doi.org/10.1007/BF00919648

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  • DOI: https://doi.org/10.1007/BF00919648

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