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Electrophoretic types of transaldolase, transketolase, and other enzymes in bifidobacteria

  • Biochemistry
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Abstract

The technique of starch-gel electrophoresis with staining for transaldolase, transketolase, 6-phosphogluconate dehydrogenase, and aldolase, was used to compare 49 representative strains of the genus Bifidobacterium, the deoxyribonucleic acid homology relationships of which were known. The zymograms obtained with fructose-6-phosphate as substrate for staining were also recorded and compared. Parallel experiments were made with spectrophotometric techniques to evaluate the specificity of the staining for transaldolase and transketolase. In each of the enzymatic activities investigated, isozymes were revealed by this technique. Their distribution is discussed.

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Authors and Affiliations

  1. Istituto di Microbiologia Agraria, Universitá di Bologna, Italia

    V. Scardovi & B. Sgorbati

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  1. V. Scardovi
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  2. B. Sgorbati
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Scardovi, V., Sgorbati, B. Electrophoretic types of transaldolase, transketolase, and other enzymes in bifidobacteria. Antonie van Leeuwenhoek 40, 427–440 (1974). https://doi.org/10.1007/BF00399355

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  • DOI: https://doi.org/10.1007/BF00399355

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