Summary
An amber mutation has been induced in the gene rpsA (which codes fo ribosomal protein S1) of Escherichia coli K-12 strain in the presence of an amber suppressor (supD) and mutations sueA, sueB and sueC that additively enhance the efficiency of suppression. That the amber mutation has occurred in the gene rpsA was confirmed by complementation with a plasmid which carried the wild-type allele of rpsA. The mutation is lethal in the absence of an amber suppressor, indicating that ribosomal protein S1 is indispensable to E. coli.
Similar content being viewed by others
References
Christiansen L, Pedersen S (1981) Cloning, restriction endonuclease mapping and post-transcriptional regulation of rpsA, the structural sene for ribosomal protein S1. Mol Gen Genet 181:548–551
Isono K, Isono S (1976) Lack of ribosomal protein S1 in Bacillus stearothermophilus. Proc Natl Acad Sci USA 73:767–770
Kimura M, Foulaki K, Subramanian A, Wittmann-Liebold B (1982) Primary structure of Escherichia coli ribosomal protein S1 and features of its functional domains. Eur J Biochem 123:37–53
Kimura M, Miki T, Hiraga S, Nagata T, Yura T (1979) Conditionally lethal amber mutations in the dnaA region of the Escherichia coli chromosome that affect chromosome replication. J Bacteriol 140:825–834
Kitakawa M, Blumenthal L, Isono K (1980) Isolation and characterization of specialized transducing λ phages carrying ribosomal protein genes of Escherichia coli. Mol Gen Genet 180:343–349
Murray NE, Brammar WJ, Murray K (1977) Lambdoid phage that simplify the recovery of in vitro recombinants. Mol Gen Genet 150:53–61
Oeschger MP, Oeschger NS, Wiprud GT, Woods SL (1980) High efficiency temperature-sensitive amber suppressor strains of Escherichia coli: isolation of strains with suppressor-enhancing mutations. Mol Gen Genet 177:545–552
Oeschger MP, Wiprud GT (1980) High efficiency temperature-sensitive amber suppressor strains of Escherichia coli K-12. Mol Gen Genet 178:293–299
Russell RL, Abelson JN, Landy A, Gefter ML, Brenner S, Smith JD (1970) Duplicate genes for tyrosine transfer RNA in Escherichia coli. J Mol Biol 47:1–13
Schnier J, Isono K (1982) The DNA sequence of the gene rpsA of Escherichia coli coding for ribosomal protein S1. Nucl Acids Res (in press)
Schnier J, Kimura M, Foulaki K, Subramanian AR, Isono K, Wittmann-Liebold B (1981) The primary structure of Escherichia coli ribosomal protein S1 and of its gene rpsA. Proc Natl Acad Sci USA 79:1008–1011
Schrenk WJ, Weisberg RA (1975) A simple method for making new transducing lines of coliphage λ. Mol Gen Genet 137:101–107
Steitz JA, Jakes K (1975) How ribosomes select intiator regions in mRNA: Base pair formation between the 3′ terminus of 169 rRNA and the mRNA during initiation of protein synthesis in Escherichia coli. Proc Natl Acad Sci USA 72:4724–4728
Subramanian AR, Mizushima S (1979) Characterization of a mutant form of ribosomal protein S1 from Escherichia coli. J Biol Chem 254:4309–4312
Szer W, Hermoso JM, Boublik M (1976) Destabilization of the secondary structure of RNA by ribosomal protein S1 from Escherichia coli. Biochem Biophys Res Commun 70:957–964
Wahba AJ, Miller MJ, Nieveleau A, Landers TA, Carmichael GG, Weber K, Hawley DA, Slobin LI (1974) Subunit I of Qβ replicase and 30S ribosomal protein S1 of Escherichia coli: Evidence for the identity of the two proteins. J Biol Chem 249:3314–3316
Author information
Authors and Affiliations
Additional information
Communicated by A. Böck
Rights and permissions
About this article
Cite this article
Kitakawa, M., Isono, K. An amber mutation in the gene rpsA for ribosomal protein S1 in Escherichia coli . Mol Gen Genet 185, 445–447 (1982). https://doi.org/10.1007/BF00334137
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00334137