Abstract
We have determined the nucleotide sequence of the 3′-terminal half of the RNA genome of Murray Valley encephalitis virus (MVE) using seven overlapping cDNA clones; an estimated 80–90 nucleotides at the extreme 3′-end remain to be sequenced. In conjunction with previous sequence data for the 5′ half (16), we can conclude that the MVE genome contains a long open reading frame of 10,302 nucleotides that encodes a polyprotein of 3434 residues. Comparison of the MVE deduced amino acid sequence with that of other flaviviruses shows that MVE is most closely related to Japanese encephalitis virus, consistent with serological studies. Using N-terminal amino acid sequence analysis, three nonstructural proteins (NS1, NS3, and NS5) have been identified and mapped on the MVE genome. MVE NS3 contains sequence motifs suggesting that its amino terminus may function as a serine protease. The central region of NS3 (in the linear amino acid sequence) has motifs that are found in NTP-binding proteins and helicases. MVE NS5 contains a conserved Gly-Asp-Asp sequence that is thought to be essential for RNA-dependent RNA polymerases.
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CalisherC.H., KarabatsosN., DalrympleJ.M., ShopeR.E., PorterfieldJ.S., WestawayE.G. and BrandtW.E. J Gen Virol 70, 37–43, 1989.
MonathT.P. in SchlesingerS. and SchlesingerM.J. (eds.) The Togaviridae and Flaviviridae, Plenum, New York, 1986, pp. 375–440.
BergeT.O., International Catalogue of Arboviruses Including Certain Other Viruses of Vertebrates, 2nd ed., Dept. of Health, Education and Welfare, Bethesda, MD, 1975.
MarshallI.D. in MonathT.P. (ed.) The Arboviruses: Epidemiology and Ecology, Vol. III, CRC Press, Boca Raton, FL, 1988, pp. 151–189.
RiceC.M., StraussE.G. and StraussJ.H. in SchlesingerS. and SchlesingerM.J. (eds.) The Togaviridae and Flaviviridae, Plenum, New York, 1986, pp. 279–326.
BrintonM.A. in SchlesingerS. and SchlesingerM.J. (eds.) The Togaviridae and Flaviviridae, Plenum, New York, 1986, pp. 327–374.
WestawayE.G., Adv Virus Res 33, 45–90, 1987.
CastleE., NowakT., LeidnerU., WenglerG. and WenglerG., Virology 145, 227–236, 1985.
CastleE., LeidnerU., NowakT., WenglerG. and WenglerG., Virology 149, 10–26, 1986.
WenglerG., CastleE., LeidnerU., NowakT. and WenglerG., Virology 147, 264–274, 1985.
CoiaG., ParkerM.D., SpeightG., ByrneM.E. and WestawayE.G. J Gen Virol 69, 1–21, 1988.
TrentD.W., KinneyR.M., JohnsonB.J.B., VorndamA.V., GrantJ.A., DeubelV., RiceC.M. and HahnC., Virology 156, 293–304, 1987.
McAdaP.C., MasonP.W., SchmaljohnC.S., DalrympleJ.M., MasonT.L. and FournierM.L., Virology 158, 348–360, 1987.
HashimotoH., NomotoA., WatanabeK., MoriT., TakezawaT., AizawaC., TakegamiT. and HiramatsuK., Virus Genes 1, 305–317, 1988.
SumiyoshiH., MoriC. FukeI., MoritaK., KuharaS., KondouJ., KikuchiY., NagamatuH. and IgarashiA., Virology 161, 497–510, 1987.
DalgarnoL., TrentD.W., StraussJ.H. and RiceC.M., J Mol Biol 187, 309–323, 1986.
HahnC.S., HahnY.S., RiceC.M., LeeE., DalgarnoL., StraussE.G. and StraussJ.H., J Mol Biol 198, 35–41, 1987.
IgarashiA., J Gen Virol 40, 531–544, 1978.
RiceC.M., DalgarnoL., GallerR., HahnY.S., StraussE.G. and StraussJ.H. in BauerH., KlenkH.D. and ScholtissekCh. (eds.) Modern Trends in Virology, Springer-Verlag, Berlin, 1988, pp. 83–97.
RiceC.M., LenchesE.M., EddyS.E., ShinS.J., SheetsR.L. and StraussJ.H., Science 229, 726–733, 1985.
MaxamA.M. and GilbertW. in GrossmanL. and MoldaveK. (eds.) Methods in Enzymology, Vol. 65, Academic Press, New York, 1980, pp. 499–560.
SmithD.R. and CalvoJ.M., Nucleic Acids Res 8, 2255–2274, 1981.
RiceC.M. and StraussJ.H., J Mol Biol 154, 325–348, 1982.
LaemmliU.K., Nature 227, 680–685, 1970.
BonnerW.M. and LaskeyR.A., Eur J Biochem 46, 83–88, 1974.
HunkapillerM.W., LujanE., OstranderF. and HoodL.E. in HirsC.H.W. and TimasheffS.N. (eds.) Methods in Enzymology, Vol. 91, Academic Press, New York, 1983, pp. 227–236.
HewickR.M., HunkapillerM.W., HoodL.E. and DreyerW.J., J Biol Chem 256, 7990–7997, 1981.
HunkapillerM.W. and HoodL.E. in HirsC.H.W. and TimasheffS.N. (eds.) Methods in Enzymology, Vol. 91, Academic Press, New York, 1983, pp. 486–494.
RiceC.M., AebersoldR., TeplowD.B., PataJ., BellJ.R., VorndamA.V., TrentD.W., BrandrissM.W., SchlesingerJ.J. and StraussJ.H. Virology 151, 1–9, 1986.
LobigsM., MarshallI.D., WeirR.C. and DalgarnoL., Aust J Exp Biol Med Sci 64, 571–585, 1986.
SpeightG., CoiaG., ParkerM.D. and WestawayE.G., J Gen Virol 69, 23–34, 1988.
SpeightG. and WestawayE.G., Virology 170, 299–301, 1989.
WestawayE.G., J Gen Virol 27, 283–292, 1975.
SpeightG. and WestawayE.G., J Gen Virol 70, 2209–2214, 1989.
MasonP.W., McAdaP.C., MasonT.L. and FournierM.J., Virology 161, 262–267, 1987.
BiedrzyckaA., CauchiM.R., BartholomeuszA., GormanJ.J. and WrightP.J., J Gen Virol 68, 1317–1326, 1987.
MackowE., MakinoY., ZhaoB., ZhangY.-M., MarkoffL., Buckler-WhiteA., GuilerM., ChanockR. and LaiC.-J., Virology 159, 217–228, 1987.
ZhaoB., MackowE., Buckler-WhiteA., MarkoffL., ChanockR.M., LaiC. and MakinoY., Virology 155, 77–88, 1986.
MandlC.W., HeinzF.X. and KunzC., Virology 166, 197–205, 1988.
VonHeijneG., J Mol Biol 184, 99–105, 1985.
GorbalenyaA.E., KooninE.V., DonchenkoA.P. and BlinovV.M., Nucleic Acids Res 17, 4713–4729, 1989.
HahnY.S., GallerR., HunkapillerT., DalrympleJ.M., StraussJ.H. and StraussE.G., Virology 162, 167–180, 1988.
KamerG. and ArgosP., Nucleic Acids Res 12, 7269–7282, 1984.
BazanJ.F. and FletterickR.J., Virology 171, 637–639, 1989.
GorbalenyaA.E., DonchenkoA.P., KooninE.V. and BlinovV.M., Nucleic Acids Res 17, 3889–3897, 1989.
HodgmanT.C., Nature 333, 22–23, 1988.
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Lee, E., Fernon, C., Simpson, R. et al. Sequence of the 3′ half of the Murray Valley encephalitis virus genome and mapping of the nonstructural proteins NS1, NS3, and NS5. Virus Genes 4, 197–213 (1990). https://doi.org/10.1007/BF00265630
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DOI: https://doi.org/10.1007/BF00265630