Abstract
Recent development in total internal reflection fluorescence microscopy (TIRFM) has made it possible to directly monitor the behaviors of biomolecules at the single-molecule level both in vitro and in living cells. This technique has opened a new era in bioscience, so-called single-molecule nanobioscience. In this review, we will summarize the theoretical basis of TIRFM, explain the instruments of TIRFM for single-molecules imaging, and discuss some novel applications of TIRFM to single-molecule nanobioscience.
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Wazawa, T., Ueda, M. Total Internal Reflection Fluorescence Microscopy in Single Molecule Nanobioscience. In: Rietdorf, J. (eds) Microscopy Techniques. Advances in Biochemical Engineering, vol 95. Springer, Berlin, Heidelberg. https://doi.org/10.1007/b102211
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DOI: https://doi.org/10.1007/b102211
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Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-23698-6
Online ISBN: 978-3-540-31545-2
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